Restriction map for
pF1KB6458
(Linear) (Six-base) MAPSORT of: KIBB6458.seq Check: 3884 from: 1 to: 1053
KIBB6458.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 224 1053
Size: 224 829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 759 992 1053
Size: 259 500 233 61
Fragments arranged by size:
500 259 233 61
AhdI GACnn_n'nnGTC
Cuts at: 0 192 1053
Size: 192 861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 775 785 1053
Size: 775 10 268
Fragments arranged by size:
775 268 10
ApaI G_GGCC'C
Cuts at: 0 721 1053
Size: 721 332
ApoI r'AATT_y
Cuts at: 0 323 1053
Size: 323 730
AvaI C'yCGr_G
Cuts at: 0 203 1053
Size: 203 850
BamHI G'GATC_C
Cuts at: 0 645 1053
Size: 645 408
BanII G_rGCy'C
Cuts at: 0 721 1053
Size: 721 332
BbvCI CC'TCA_GC
Cuts at: 0 317 1053
Size: 317 736
BglII A'GATC_T
Cuts at: 0 258 1053
Size: 258 795
Bme1580I G_kGCm'C
Cuts at: 0 721 1053
Size: 721 332
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 688 1053
Size: 469 219 365
Fragments arranged by size:
469 365 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1053
Size: 757 296
Bpu10I CC'TnA_GC
Cuts at: 0 317 1053
Size: 317 736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1053
Size: 433 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 348 1053
Size: 348 705
BsaBI GATnn'nnATC
Cuts at: 0 644 1053
Size: 644 409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 562 1053
Size: 487 75 491
Fragments arranged by size:
491 487 75
BseYI C'CCAG_C
Cuts at: 0 827 1053
Size: 827 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 929 1053
Size: 200 729 124
Fragments arranged by size:
729 200 124
Bsp1286I G_dGCh'C
Cuts at: 0 721 1053
Size: 721 332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 1053
Size: 49 1004
BsrDI GCAATG_nn'
Cuts at: 0 394 1053
Size: 394 659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 835 1053
Size: 835 218
BstYI r'GATC_y
Cuts at: 0 258 645 1053
Size: 258 387 408
Fragments arranged by size:
408 387 258
BtgI C'CryG_G
Cuts at: 0 338 843 1053
Size: 338 505 210
Fragments arranged by size:
505 338 210
BtsI GCAGTG_nn'
Cuts at: 0 509 692 935 1053
Size: 509 183 243 118
Fragments arranged by size:
509 243 183 118
ClaI AT'CG_AT
Cuts at: 0 332 1053
Size: 332 721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 178 213 1053
Size: 178 35 840
Fragments arranged by size:
840 178 35
DraIII CAC_nnn'GTG
Cuts at: 0 814 1053
Size: 814 239
EaeI y'GGCC_r
Cuts at: 0 1018 1053
Size: 1018 35
EarI CTCTTCn'nnn_
Cuts at: 0 235 577 1053
Size: 235 342 476
Fragments arranged by size:
476 342 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 757 759 992 1053
Size: 259 498 2 233 61
Fragments arranged by size:
498 259 233 61 2
EcoO109I rG'GnC_Cy
Cuts at: 0 717 718 795 1053
Size: 717 1 77 258
Fragments arranged by size:
717 258 77 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 1053
Size: 162 891
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 1053
Size: 178 32 843
Fragments arranged by size:
843 178 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1053
Size: 281 772
MscI TGG'CCA
Cuts at: 0 1020 1053
Size: 1020 33
MslI CAynn'nnrTG
Cuts at: 0 86 1053
Size: 86 967
MspA1I CmG'CkG
Cuts at: 0 36 779 1053
Size: 36 743 274
Fragments arranged by size:
743 274 36
NcoI C'CATG_G
Cuts at: 0 843 1053
Size: 843 210
NspI r_CATG'y
Cuts at: 0 762 1053
Size: 762 291
PasI CC'CwG_GG
Cuts at: 0 729 1053
Size: 729 324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 1053
Size: 7 32 1014
Fragments arranged by size:
1014 32 7
PspOMI G'GGCC_C
Cuts at: 0 717 1053
Size: 717 336
PstI C_TGCA'G
Cuts at: 0 687 960 1053
Size: 687 273 93
Fragments arranged by size:
687 273 93
SfcI C'TryA_G
Cuts at: 0 683 956 1053
Size: 683 273 97
Fragments arranged by size:
683 273 97
SmlI C'TyrA_G
Cuts at: 0 448 1053
Size: 448 605
SphI G_CATG'C
Cuts at: 0 762 1053
Size: 762 291
StyI C'CwwG_G
Cuts at: 0 843 1053
Size: 843 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 418 1053
Size: 418 635
TatI w'GTAC_w
Cuts at: 0 268 1053
Size: 268 785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1053
Size: 824 32 197
Fragments arranged by size:
824 197 32
Enzymes that do cut:
AccI AcuI AhdI AlfI ApaI ApoI AvaI BamHI
BanII BbvCI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BseRI BseYI BsgI Bsp1286I BspMI BsrDI
BstXI BstYI BtgI BtsI ClaI CspCI DraIII EaeI
EarI Eco57MI EcoO109I FalI Hin4I MmeI MscI MslI
MspA1I NcoI NspI PasI PpiI PspOMI PstI SfcI
SmlI SphI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BsaAI BsaHI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI