Restriction map for
pF1KB6453
(Linear) (Six-base) MAPSORT of: KIBB6453.seq Check: 6676 from: 1 to: 954
KIBB6453.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 607 954
Size: 307 300 347
Fragments arranged by size:
347 307 300
AfeI AGC'GCT
Cuts at: 0 559 954
Size: 559 395
AlwNI CAG_nnn'CTG
Cuts at: 0 330 954
Size: 330 624
ApaI G_GGCC'C
Cuts at: 0 124 954
Size: 124 830
ApoI r'AATT_y
Cuts at: 0 481 954
Size: 481 473
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 532 565 954
Size: 532 33 389
Fragments arranged by size:
532 389 33
BanI G'GyrC_C
Cuts at: 0 761 839 954
Size: 761 78 115
Fragments arranged by size:
761 115 78
BanII G_rGCy'C
Cuts at: 0 50 124 954
Size: 50 74 830
Fragments arranged by size:
830 74 50
BbeI G_GCGC'C
Cuts at: 0 843 954
Size: 843 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 954
Size: 41 913
BclI T'GATC_A
Cuts at: 0 453 921 954
Size: 453 468 33
Fragments arranged by size:
468 453 33
BglII A'GATC_T
Cuts at: 0 188 954
Size: 188 766
Bme1580I G_kGCm'C
Cuts at: 0 124 415 954
Size: 124 291 539
Fragments arranged by size:
539 291 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 158 954
Size: 158 796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 954
Size: 565 389
Bpu10I CC'TnA_GC
Cuts at: 0 49 954
Size: 49 905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 954
Size: 169 785
BsaI GGTCTCn'nnnn_
Cuts at: 0 280 620 954
Size: 280 340 334
Fragments arranged by size:
340 334 280
BsaHI Gr'CG_yC
Cuts at: 0 840 954
Size: 840 114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 460 954
Size: 457 3 494
Fragments arranged by size:
494 457 3
BseYI C'CCAG_C
Cuts at: 0 123 560 954
Size: 123 437 394
Fragments arranged by size:
437 394 123
BsiEI CG_ry'CG
Cuts at: 0 86 954
Size: 86 868
BsiHKAI G_wGCw'C
Cuts at: 0 50 954
Size: 50 904
BsmI GAATG_Cn'
Cuts at: 0 690 819 954
Size: 690 129 135
Fragments arranged by size:
690 135 129
Bsp1286I G_dGCh'C
Cuts at: 0 50 124 415 954
Size: 50 74 291 539
Fragments arranged by size:
539 291 74 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 183 885 954
Size: 183 702 69
Fragments arranged by size:
702 183 69
BsrBI CCG'CTC
Cuts at: 0 25 954
Size: 25 929
BsrFI r'CCGG_y
Cuts at: 0 551 954
Size: 551 403
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 954
Size: 738 216
BstYI r'GATC_y
Cuts at: 0 188 896 954
Size: 188 708 58
Fragments arranged by size:
708 188 58
BtsI GCAGTG_nn'
Cuts at: 0 924 954
Size: 924 30
DraIII CAC_nnn'GTG
Cuts at: 0 166 954
Size: 166 788
EaeI y'GGCC_r
Cuts at: 0 83 954
Size: 83 871
EagI C'GGCC_G
Cuts at: 0 83 954
Size: 83 871
EarI CTCTTCn'nnn_
Cuts at: 0 67 954
Size: 67 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 954
Size: 46 908
EcoICRI GAG'CTC
Cuts at: 0 48 954
Size: 48 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 565 607 954
Size: 307 258 42 347
Fragments arranged by size:
347 307 258 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 521 954
Size: 133 388 433
Fragments arranged by size:
433 388 133
EcoO109I rG'GnC_Cy
Cuts at: 0 120 138 591 909 954
Size: 120 18 453 318 45
Fragments arranged by size:
453 318 120 45 18
HaeII r_GCGC'y
Cuts at: 0 561 711 843 954
Size: 561 150 132 111
Fragments arranged by size:
561 150 132 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 801 833 954
Size: 801 32 121
Fragments arranged by size:
801 121 32
KasI G'GCGC_C
Cuts at: 0 839 954
Size: 839 115
MspA1I CmG'CkG
Cuts at: 0 316 954
Size: 316 638
NarI GG'CG_CC
Cuts at: 0 840 954
Size: 840 114
NdeI CA'TA_TG
Cuts at: 0 510 954
Size: 510 444
PpuMI rG'GwC_Cy
Cuts at: 0 138 954
Size: 138 816
PspOMI G'GGCC_C
Cuts at: 0 120 954
Size: 120 834
PstI C_TGCA'G
Cuts at: 0 734 954
Size: 734 220
PvuII CAG'CTG
Cuts at: 0 316 954
Size: 316 638
SacI G_AGCT'C
Cuts at: 0 50 954
Size: 50 904
SfcI C'TryA_G
Cuts at: 0 730 954
Size: 730 224
SfoI GGC'GCC
Cuts at: 0 841 954
Size: 841 113
SmlI C'TyrA_G
Cuts at: 0 184 954
Size: 184 770
StyI C'CwwG_G
Cuts at: 0 668 711 905 954
Size: 668 43 194 49
Fragments arranged by size:
668 194 49 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 954
Size: 177 777
XmnI GAAnn'nnTTC
Cuts at: 0 572 954
Size: 572 382
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI BaeI BanI BanII
BbeI BbsI BclI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MspA1I NarI NdeI PpuMI PspOMI
PstI PvuII SacI SfcI SfoI SmlI StyI TaqII
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BbvCI BcgI BciVI BfrBI BglI
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsgI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI