Restriction map for
pF1KB6452
(Linear) (Six-base) MAPSORT of: KIBB6452.seq Check: 3704 from: 1 to: 1050
KIBB6452.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 35 1050
Size: 35 1015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 796 1050
Size: 232 564 254
Fragments arranged by size:
564 254 232
AflIII A'CryG_T
Cuts at: 0 644 1050
Size: 644 406
AhdI GACnn_n'nnGTC
Cuts at: 0 315 877 1050
Size: 315 562 173
Fragments arranged by size:
562 315 173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 683 715 1050
Size: 683 32 335
Fragments arranged by size:
683 335 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 939 1050
Size: 384 555 111
Fragments arranged by size:
555 384 111
ApaI G_GGCC'C
Cuts at: 0 30 1050
Size: 30 1020
ApoI r'AATT_y
Cuts at: 0 541 1050
Size: 541 509
AseI AT'TA_AT
Cuts at: 0 173 1050
Size: 173 877
AvaI C'yCGr_G
Cuts at: 0 29 93 1050
Size: 29 64 957
Fragments arranged by size:
957 64 29
BanII G_rGCy'C
Cuts at: 0 30 608 1050
Size: 30 578 442
Fragments arranged by size:
578 442 30
BglI GCCn_nnn'nGGC
Cuts at: 0 952 1050
Size: 952 98
BglII A'GATC_T
Cuts at: 0 897 1050
Size: 897 153
Bme1580I G_kGCm'C
Cuts at: 0 30 1050
Size: 30 1020
BmgBI CAC'GTC
Cuts at: 0 90 1050
Size: 90 960
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 1050
Size: 81 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1050
Size: 277 773
Bpu10I CC'TnA_GC
Cuts at: 0 557 1050
Size: 557 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 1050
Size: 567 483
BsaI GGTCTCn'nnnn_
Cuts at: 0 1039 1050
Size: 1039 11
BsaWI w'CCGG_w
Cuts at: 0 401 1050
Size: 401 649
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 590 620 1050
Size: 590 30 430
Fragments arranged by size:
590 430 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 598 1050
Size: 72 526 452
Fragments arranged by size:
526 452 72
BseYI C'CCAG_C
Cuts at: 0 98 842 998 1050
Size: 98 744 156 52
Fragments arranged by size:
744 156 98 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1050
Size: 1028 22
BsiEI CG_ry'CG
Cuts at: 0 14 1050
Size: 14 1036
BsiHKAI G_wGCw'C
Cuts at: 0 608 965 1050
Size: 608 357 85
Fragments arranged by size:
608 357 85
Bsp1286I G_dGCh'C
Cuts at: 0 30 608 965 1050
Size: 30 578 357 85
Fragments arranged by size:
578 357 85 30
BspHI T'CATG_A
Cuts at: 0 488 1050
Size: 488 562
BsrDI GCAATG_nn'
Cuts at: 0 734 1050
Size: 734 316
BssSI C'ACGA_G
Cuts at: 0 610 1050
Size: 610 440
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1015 1050
Size: 1015 35
BstBI TT'CG_AA
Cuts at: 0 129 684 1050
Size: 129 555 366
Fragments arranged by size:
555 366 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 1050
Size: 747 303
BstYI r'GATC_y
Cuts at: 0 380 798 897 1050
Size: 380 418 99 153
Fragments arranged by size:
418 380 153 99
Bsu36I CC'TnA_GG
Cuts at: 0 887 1050
Size: 887 163
BtgI C'CryG_G
Cuts at: 0 14 702 956 1050
Size: 14 688 254 94
Fragments arranged by size:
688 254 94 14
EaeI y'GGCC_r
Cuts at: 0 11 1050
Size: 11 1039
EagI C'GGCC_G
Cuts at: 0 11 1050
Size: 11 1039
EcoICRI GAG'CTC
Cuts at: 0 606 1050
Size: 606 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 277 796 1050
Size: 232 45 519 254
Fragments arranged by size:
519 254 232 45
EcoO109I rG'GnC_Cy
Cuts at: 0 26 309 891 1050
Size: 26 283 582 159
Fragments arranged by size:
582 283 159 26
EcoRI G'AATT_C
Cuts at: 0 541 1050
Size: 541 509
EcoRV GAT'ATC
Cuts at: 0 399 816 1050
Size: 399 417 234
Fragments arranged by size:
417 399 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 590 622 863 895 1050
Size: 301 32 257 32 241 32 155
Fragments arranged by size:
301 257 241 155 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 467 1050
Size: 467 583
MspA1I CmG'CkG
Cuts at: 0 16 1050
Size: 16 1034
NcoI C'CATG_G
Cuts at: 0 702 956 1050
Size: 702 254 94
Fragments arranged by size:
702 254 94
NspI r_CATG'y
Cuts at: 0 648 1050
Size: 648 402
PciI A'CATG_T
Cuts at: 0 644 1050
Size: 644 406
PpuMI rG'GwC_Cy
Cuts at: 0 309 1050
Size: 309 741
PspOMI G'GGCC_C
Cuts at: 0 26 1050
Size: 26 1024
SacI G_AGCT'C
Cuts at: 0 608 1050
Size: 608 442
SacII CC_GC'GG
Cuts at: 0 17 1050
Size: 17 1033
ScaI AGT'ACT
Cuts at: 0 70 1050
Size: 70 980
SmaI CCC'GGG
Cuts at: 0 31 1050
Size: 31 1019
SmlI C'TyrA_G
Cuts at: 0 582 1050
Size: 582 468
StyI C'CwwG_G
Cuts at: 0 702 740 956 1050
Size: 702 38 216 94
Fragments arranged by size:
702 216 94 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 980 1050
Size: 980 70
TatI w'GTAC_w
Cuts at: 0 68 563 1050
Size: 68 495 487
Fragments arranged by size:
495 487 68
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 954 1050
Size: 954 96
XmaI C'CCGG_G
Cuts at: 0 29 1050
Size: 29 1021
Enzymes that do cut:
AccI AcuI AflIII AhdI AloI AlwNI ApaI ApoI
AseI AvaI BanII BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI EaeI EagI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV Hin4I MslI MspA1I NcoI
NspI PciI PpuMI PspOMI SacI SacII ScaI SmaI
SmlI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoNI FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI