Restriction map for
pF1KB6447
(Linear) (Six-base) MAPSORT of: KIBB6447.seq Check: 8883 from: 1 to: 726
KIBB6447.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 457 619 726
Size: 277 180 162 107
Fragments arranged by size:
277 180 162 107
AgeI A'CCGG_T
Cuts at: 0 671 726
Size: 671 55
AlwNI CAG_nnn'CTG
Cuts at: 0 372 423 726
Size: 372 51 303
Fragments arranged by size:
372 303 51
BamHI G'GATC_C
Cuts at: 0 311 726
Size: 311 415
BanI G'GyrC_C
Cuts at: 0 547 726
Size: 547 179
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 538 726
Size: 32 506 188
Fragments arranged by size:
506 188 32
BfrBI ATG'CAT
Cuts at: 0 162 726
Size: 162 564
BlpI GC'TnA_GC
Cuts at: 0 424 726
Size: 424 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 244 726
Size: 244 482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 154 430 643 726
Size: 64 90 276 213 83
Fragments arranged by size:
276 213 90 83 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 726
Size: 334 392
BsaHI Gr'CG_yC
Cuts at: 0 106 726
Size: 106 620
BsaWI w'CCGG_w
Cuts at: 0 671 719 726
Size: 671 48 7
Fragments arranged by size:
671 48 7
BseYI C'CCAG_C
Cuts at: 0 224 500 726
Size: 224 276 226
Fragments arranged by size:
276 226 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 726
Size: 196 530
BsiEI CG_ry'CG
Cuts at: 0 676 726
Size: 676 50
BsiHKAI G_wGCw'C
Cuts at: 0 416 726
Size: 416 310
Bsp1286I G_dGCh'C
Cuts at: 0 416 726
Size: 416 310
BspEI T'CCGG_A
Cuts at: 0 719 726
Size: 719 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 726
Size: 227 499
BsrBI CCG'CTC
Cuts at: 0 63 726
Size: 63 663
BsrFI r'CCGG_y
Cuts at: 0 671 726
Size: 671 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 125 558 726
Size: 125 433 168
Fragments arranged by size:
433 168 125
BstYI r'GATC_y
Cuts at: 0 311 726
Size: 311 415
EaeI y'GGCC_r
Cuts at: 0 32 560 726
Size: 32 528 166
Fragments arranged by size:
528 166 32
EarI CTCTTCn'nnn_
Cuts at: 0 14 580 726
Size: 14 566 146
Fragments arranged by size:
566 146 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 154 277 430 457 619 643 726
Size: 64 90 123 153 27 162 24 83
Fragments arranged by size:
162 153 123 90 83 64 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 469 601 726
Size: 469 132 125
Fragments arranged by size:
469 132 125
HaeII r_GCGC'y
Cuts at: 0 403 431 726
Size: 403 28 295
Fragments arranged by size:
403 295 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 103 123 135 726
Size: 91 12 20 12 591
Fragments arranged by size:
591 91 20 12 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 656 726
Size: 89 567 70
Fragments arranged by size:
567 89 70
MscI TGG'CCA
Cuts at: 0 562 726
Size: 562 164
MslI CAynn'nnrTG
Cuts at: 0 556 726
Size: 556 170
MspA1I CmG'CkG
Cuts at: 0 420 642 726
Size: 420 222 84
Fragments arranged by size:
420 222 84
NsiI A_TGCA'T
Cuts at: 0 164 726
Size: 164 562
NspI r_CATG'y
Cuts at: 0 366 726
Size: 366 360
PfoI T'CCnGG_A
Cuts at: 0 290 726
Size: 290 436
PstI C_TGCA'G
Cuts at: 0 53 74 726
Size: 53 21 652
Fragments arranged by size:
652 53 21
PvuII CAG'CTG
Cuts at: 0 642 726
Size: 642 84
ScaI AGT'ACT
Cuts at: 0 40 726
Size: 40 686
SfcI C'TryA_G
Cuts at: 0 49 70 726
Size: 49 21 656
Fragments arranged by size:
656 49 21
SmlI C'TyrA_G
Cuts at: 0 349 726
Size: 349 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 726
Size: 646 80
TatI w'GTAC_w
Cuts at: 0 38 726
Size: 38 688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 726
Size: 129 597
XmnI GAAnn'nnTTC
Cuts at: 0 574 726
Size: 574 152
Enzymes that do cut:
AcuI AgeI AlwNI BamHI BanI BbsI BfrBI BlpI
BmrI BpmI BpuEI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstXI BstYI
EaeI EarI Eco57MI EcoNI HaeII Hin4I MmeI MscI
MslI MspA1I NsiI NspI PfoI PstI PvuII ScaI
SfcI SmlI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI