Restriction map for
pF1KB6446
(Linear) (Six-base) MAPSORT of: KIBB6446.seq Check: 5949 from: 1 to: 1041
KIBB6446.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 49 496 570 1041
Size: 49 447 74 471
Fragments arranged by size:
471 447 74 49
Acc65I G'GTAC_C
Cuts at: 0 974 1041
Size: 974 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1041
Size: 298 743
AhdI GACnn_n'nnGTC
Cuts at: 0 910 1041
Size: 910 131
AleI CACnn'nnGTG
Cuts at: 0 793 1041
Size: 793 248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 1041
Size: 847 194
AvaI C'yCGr_G
Cuts at: 0 404 569 998 1041
Size: 404 165 429 43
Fragments arranged by size:
429 404 165 43
BanI G'GyrC_C
Cuts at: 0 37 706 784 974 1041
Size: 37 669 78 190 67
Fragments arranged by size:
669 190 78 67 37
BanII G_rGCy'C
Cuts at: 0 224 405 413 576 1041
Size: 224 181 8 163 465
Fragments arranged by size:
465 224 181 163 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 720 1041
Size: 720 321
BciVI GTATCCnnnnn_n'
Cuts at: 0 788 1041
Size: 788 253
BglI GCCn_nnn'nGGC
Cuts at: 0 109 238 821 1041
Size: 109 129 583 220
Fragments arranged by size:
583 220 129 109
BmgBI CAC'GTC
Cuts at: 0 887 1041
Size: 887 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1041
Size: 163 878
Bpu10I CC'TnA_GC
Cuts at: 0 85 1041
Size: 85 956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1041
Size: 229 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 666 897 1041
Size: 88 578 231 144
Fragments arranged by size:
578 231 144 88
BsaHI Gr'CG_yC
Cuts at: 0 671 1041
Size: 671 370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 356 386 1041
Size: 356 30 655
Fragments arranged by size:
655 356 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 410 413 440 478 1024 1041
Size: 139 271 3 27 38 546 17
Fragments arranged by size:
546 271 139 38 27 17 3
BseYI C'CCAG_C
Cuts at: 0 99 688 962 1041
Size: 99 589 274 79
Fragments arranged by size:
589 274 99 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1041
Size: 933 108
BsiEI CG_ry'CG
Cuts at: 0 609 1041
Size: 609 432
BsiHKAI G_wGCw'C
Cuts at: 0 131 341 405 487 1041
Size: 131 210 64 82 554
Fragments arranged by size:
554 210 131 82 64
BsmI GAATG_Cn'
Cuts at: 0 522 543 1041
Size: 522 21 498
Fragments arranged by size:
522 498 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 871 1041
Size: 871 170
Bsp1286I G_dGCh'C
Cuts at: 0 131 224 341 405 413 487 576 1041
Size: 131 93 117 64 8 74 89 465
Fragments arranged by size:
465 131 117 93 89 74 64 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 496 570 1041
Size: 49 447 74 471
Fragments arranged by size:
471 447 74 49
BsrFI r'CCGG_y
Cuts at: 0 539 1041
Size: 539 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 528 795 970 1041
Size: 501 27 267 175 71
Fragments arranged by size:
501 267 175 71 27
BstYI r'GATC_y
Cuts at: 0 509 1041
Size: 509 532
Bsu36I CC'TnA_GG
Cuts at: 0 818 1041
Size: 818 223
DraIII CAC_nnn'GTG
Cuts at: 0 135 1041
Size: 135 906
EaeI y'GGCC_r
Cuts at: 0 606 1041
Size: 606 435
EagI C'GGCC_G
Cuts at: 0 606 1041
Size: 606 435
EarI CTCTTCn'nnn_
Cuts at: 0 449 467 1041
Size: 449 18 574
Fragments arranged by size:
574 449 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 666 1041
Size: 666 375
EcoICRI GAG'CTC
Cuts at: 0 403 1041
Size: 403 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 298 1041
Size: 163 135 743
Fragments arranged by size:
743 163 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 1041
Size: 460 581
EcoO109I rG'GnC_Cy
Cuts at: 0 196 958 1041
Size: 196 762 83
Fragments arranged by size:
762 196 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 896 928 1041
Size: 896 32 113
Fragments arranged by size:
896 113 32
KpnI G_GTAC'C
Cuts at: 0 978 1041
Size: 978 63
MslI CAynn'nnrTG
Cuts at: 0 793 889 1041
Size: 793 96 152
Fragments arranged by size:
793 152 96
MspA1I CmG'CkG
Cuts at: 0 624 947 966 1041
Size: 624 323 19 75
Fragments arranged by size:
624 323 75 19
NaeI GCC'GGC
Cuts at: 0 541 1041
Size: 541 500
NgoMIV G'CCGG_C
Cuts at: 0 539 1041
Size: 539 502
NotI GC'GGCC_GC
Cuts at: 0 606 1041
Size: 606 435
PfoI T'CCnGG_A
Cuts at: 0 806 1041
Size: 806 235
PpuMI rG'GwC_Cy
Cuts at: 0 196 958 1041
Size: 196 762 83
Fragments arranged by size:
762 196 83
PshAI GACnn'nnGTC
Cuts at: 0 863 1041
Size: 863 178
PstI C_TGCA'G
Cuts at: 0 581 1041
Size: 581 460
PvuII CAG'CTG
Cuts at: 0 624 947 966 1041
Size: 624 323 19 75
Fragments arranged by size:
624 323 75 19
SacI G_AGCT'C
Cuts at: 0 405 1041
Size: 405 636
SanDI GG'GwC_CC
Cuts at: 0 958 1041
Size: 958 83
SapI GCTCTTCn'nnn_
Cuts at: 0 467 1041
Size: 467 574
SfcI C'TryA_G
Cuts at: 0 577 1041
Size: 577 464
SmaI CCC'GGG
Cuts at: 0 571 1000 1041
Size: 571 429 41
Fragments arranged by size:
571 429 41
SmlI C'TyrA_G
Cuts at: 0 244 1041
Size: 244 797
StuI AGG'CCT
Cuts at: 0 592 1041
Size: 592 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 773 921 1041
Size: 773 148 120
Fragments arranged by size:
773 148 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 356 388 1041
Size: 121 32 203 32 653
Fragments arranged by size:
653 203 121 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 60 1041
Size: 60 981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 643 1041
Size: 643 398
XmaI C'CCGG_G
Cuts at: 0 569 998 1041
Size: 569 429 43
Fragments arranged by size:
569 429 43
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlfI AvaI BanI
BanII BbsI BciVI BglI BmgBI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I KpnI MslI MspA1I NaeI NgoMIV NotI
PfoI PpuMI PshAI PstI PvuII SacI SanDI SapI
SfcI SmaI SmlI StuI TaqII TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BsaAI BsaBI BsaWI
BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI XbaI
XhoI XmnI ZraI