(Linear) (Six-base) MAPSORT of: KIBB6441.seq  Check: 8114  from: 1  to: 942



KIBB6441.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     942 
   Size:        341     601
 
AflIII A'CryG_T

Cuts at:      0     821     942 
   Size:        821     121
 
AhdI GACnn_n'nnGTC

Cuts at:      0     261     942 
   Size:        261     681
 
ApoI r'AATT_y

Cuts at:      0     799     942 
   Size:        799     143
 
BamHI G'GATC_C

Cuts at:      0     186     942 
   Size:        186     756
 
BclI T'GATC_A

Cuts at:      0     897     942 
   Size:        897      45
 
BfrBI ATG'CAT

Cuts at:      0     884     942 
   Size:        884      58
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     656     942 
   Size:        656     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     942 
   Size:        149     793
 
Bpu10I CC'TnA_GC

Cuts at:      0     676     942 
   Size:        676     266
 
BsaAI yAC'GTr

Cuts at:      0     822     942 
   Size:        822     120
 
BsmI GAATG_Cn'

Cuts at:      0     886     942 
   Size:        886      56
 
BsrGI T'GTAC_A

Cuts at:      0     738     942 
   Size:        738     204
 
BstYI r'GATC_y

Cuts at:      0     186     942 
   Size:        186     756
 
BtgI C'CryG_G

Cuts at:      0     465     857     942 
   Size:        465     392      85

  Fragments arranged by size: 

                 465     392      85
 
DraI TTT'AAA

Cuts at:      0     573     632     942 
   Size:        573      59     310

  Fragments arranged by size: 

                 573     310      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     341     942 
   Size:        149     192     601

  Fragments arranged by size: 

                 601     192     149
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     316     942 
   Size:        316     626
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     171     203     247     279     549     581     942 
   Size:        171      32      44      32     270      32     361

  Fragments arranged by size: 

                 361     270     171      44      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     437     942 
   Size:        437     505
 
MslI CAynn'nnrTG

Cuts at:      0     502     942 
   Size:        502     440
 
NcoI C'CATG_G

Cuts at:      0     465     857     942 
   Size:        465     392      85

  Fragments arranged by size: 

                 465     392      85
 
NsiI A_TGCA'T

Cuts at:      0     886     942 
   Size:        886      56
 
SfcI C'TryA_G

Cuts at:      0     546     942 
   Size:        546     396
 
StyI C'CwwG_G

Cuts at:      0     465     857     942 
   Size:        465     392      85

  Fragments arranged by size: 

                 465     392      85
 
SwaI ATTT'AAAT

Cuts at:      0     632     942 
   Size:        632     310
 
TatI w'GTAC_w

Cuts at:      0     738     942 
   Size:        738     204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     128     160     942 
   Size:        128      32     782

  Fragments arranged by size: 

                 782     128      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      41     942 
   Size:         41     901
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     ApoI    BamHI     BclI    BfrBI     BmrI
     BpmI   Bpu10I    BsaAI     BsmI    BsrGI    BstYI     BtgI     DraI
  Eco57MI     FalI    Hin4I     MfeI     MslI     NcoI     NsiI     SfcI
     StyI     SwaI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI