Restriction map for
pF1KB6439
(Linear) (Six-base) MAPSORT of: KIBB6439.seq Check: 7533 from: 1 to: 942
KIBB6439.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 696 942
Size: 696 246
AflIII A'CryG_T
Cuts at: 0 751 942
Size: 751 191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 942
Size: 487 455
ApaLI G'TGCA_C
Cuts at: 0 525 942
Size: 525 417
ApoI r'AATT_y
Cuts at: 0 157 510 942
Size: 157 353 432
Fragments arranged by size:
432 353 157
BamHI G'GATC_C
Cuts at: 0 871 942
Size: 871 71
BanII G_rGCy'C
Cuts at: 0 392 942
Size: 392 550
BbsI GAAGACnn'nnnn_
Cuts at: 0 328 942
Size: 328 614
Bme1580I G_kGCm'C
Cuts at: 0 497 529 942
Size: 497 32 413
Fragments arranged by size:
497 413 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 576 635 915 942
Size: 576 59 280 27
Fragments arranged by size:
576 280 59 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 942
Size: 661 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 942
Size: 367 575
BsaAI yAC'GTr
Cuts at: 0 27 942
Size: 27 915
BsiHKAI G_wGCw'C
Cuts at: 0 529 942
Size: 529 413
Bsp1286I G_dGCh'C
Cuts at: 0 392 497 529 942
Size: 392 105 32 413
Fragments arranged by size:
413 392 105 32
BspHI T'CATG_A
Cuts at: 0 531 942
Size: 531 411
BsrBI CCG'CTC
Cuts at: 0 410 942
Size: 410 532
BsrDI GCAATG_nn'
Cuts at: 0 392 942
Size: 392 550
BsrFI r'CCGG_y
Cuts at: 0 453 942
Size: 453 489
BsrGI T'GTAC_A
Cuts at: 0 425 942
Size: 425 517
BstAPI GCAn_nnn'nTGC
Cuts at: 0 175 942
Size: 175 767
BstEII G'GTnAC_C
Cuts at: 0 801 942
Size: 801 141
BstYI r'GATC_y
Cuts at: 0 871 942
Size: 871 71
Bsu36I CC'TnA_GG
Cuts at: 0 419 942
Size: 419 523
BtgI C'CryG_G
Cuts at: 0 704 942
Size: 704 238
EaeI y'GGCC_r
Cuts at: 0 2 242 459 707 891 942
Size: 2 240 217 248 184 51
Fragments arranged by size:
248 240 217 184 51 2
EarI CTCTTCn'nnn_
Cuts at: 0 276 326 942
Size: 276 50 616
Fragments arranged by size:
616 276 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 942
Size: 36 906
MscI TGG'CCA
Cuts at: 0 4 244 709 893 942
Size: 4 240 465 184 49
Fragments arranged by size:
465 240 184 49 4
MslI CAynn'nnrTG
Cuts at: 0 469 942
Size: 469 473
MspA1I CmG'CkG
Cuts at: 0 518 942
Size: 518 424
NaeI GCC'GGC
Cuts at: 0 455 942
Size: 455 487
NcoI C'CATG_G
Cuts at: 0 704 942
Size: 704 238
NgoMIV G'CCGG_C
Cuts at: 0 453 942
Size: 453 489
NspI r_CATG'y
Cuts at: 0 755 787 942
Size: 755 32 155
Fragments arranged by size:
755 155 32
PciI A'CATG_T
Cuts at: 0 751 942
Size: 751 191
PstI C_TGCA'G
Cuts at: 0 183 942
Size: 183 759
SapI GCTCTTCn'nnn_
Cuts at: 0 276 942
Size: 276 666
SexAI A'CCwGG_T
Cuts at: 0 690 942
Size: 690 252
SfcI C'TryA_G
Cuts at: 0 179 942
Size: 179 763
SmlI C'TyrA_G
Cuts at: 0 676 942
Size: 676 266
StuI AGG'CCT
Cuts at: 0 812 942
Size: 812 130
StyI C'CwwG_G
Cuts at: 0 704 851 942
Size: 704 147 91
Fragments arranged by size:
704 147 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 942
Size: 19 923
TatI w'GTAC_w
Cuts at: 0 425 942
Size: 425 517
Tth111I GACn'n_nGTC
Cuts at: 0 692 942
Size: 692 250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 942
Size: 622 320
XmnI GAAnn'nnTTC
Cuts at: 0 652 942
Size: 652 290
Enzymes that do cut:
AccI AflIII AlfI ApaLI ApoI BamHI BanII BbsI
Bme1580I BmrI BpuEI BsaI BsaAI BsiHKAI Bsp1286I BspHI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI Bsu36I
BtgI EaeI EarI FalI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PstI SapI SexAI SfcI
SmlI StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BssHII
BssSI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI ZraI