Restriction map for
pF1KB6385
(Linear) (Six-base) MAPSORT of: KIBB6385.seq Check: 4442 from: 1 to: 819
KIBB6385.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 425 819
Size: 425 394
Acc65I G'GTAC_C
Cuts at: 0 383 819
Size: 383 436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 363 819
Size: 25 338 456
Fragments arranged by size:
456 338 25
AflIII A'CryG_T
Cuts at: 0 72 314 819
Size: 72 242 505
Fragments arranged by size:
505 242 72
AgeI A'CCGG_T
Cuts at: 0 536 819
Size: 536 283
AleI CACnn'nnGTG
Cuts at: 0 398 819
Size: 398 421
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 819
Size: 62 32 725
Fragments arranged by size:
725 62 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 819
Size: 576 243
AvaI C'yCGr_G
Cuts at: 0 23 81 819
Size: 23 58 738
Fragments arranged by size:
738 58 23
BanI G'GyrC_C
Cuts at: 0 160 383 661 819
Size: 160 223 278 158
Fragments arranged by size:
278 223 160 158
BanII G_rGCy'C
Cuts at: 0 23 89 338 502 622 688 701 819
Size: 23 66 249 164 120 66 13 118
Fragments arranged by size:
249 164 120 118 66 66 23 13
BbeI G_GCGC'C
Cuts at: 0 164 665 819
Size: 164 501 154
Fragments arranged by size:
501 164 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 819
Size: 539 280
BclI T'GATC_A
Cuts at: 0 647 819
Size: 647 172
BfrBI ATG'CAT
Cuts at: 0 61 819
Size: 61 758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 275 580 799 819
Size: 37 238 305 219 20
Fragments arranged by size:
305 238 219 37 20
Bpu10I CC'TnA_GC
Cuts at: 0 569 819
Size: 569 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 819
Size: 73 746
BsaAI yAC'GTr
Cuts at: 0 396 819
Size: 396 423
BsaHI Gr'CG_yC
Cuts at: 0 161 269 295 662 819
Size: 161 108 26 367 157
Fragments arranged by size:
367 161 157 108 26
BsaWI w'CCGG_w
Cuts at: 0 536 819
Size: 536 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 604 634 819
Size: 62 30 512 30 185
Fragments arranged by size:
512 185 62 30 30
BsiEI CG_ry'CG
Cuts at: 0 191 819
Size: 191 628
BsiHKAI G_wGCw'C
Cuts at: 0 688 819
Size: 688 131
Bsp1286I G_dGCh'C
Cuts at: 0 23 89 338 502 622 688 701 819
Size: 23 66 249 164 120 66 13 118
Fragments arranged by size:
249 164 120 118 66 66 23 13
BsrDI GCAATG_nn'
Cuts at: 0 636 819
Size: 636 183
BsrFI r'CCGG_y
Cuts at: 0 536 819
Size: 536 283
BstEII G'GTnAC_C
Cuts at: 0 610 819
Size: 610 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 819
Size: 288 531
BstYI r'GATC_y
Cuts at: 0 215 602 819
Size: 215 387 217
Fragments arranged by size:
387 217 215
BtgI C'CryG_G
Cuts at: 0 263 347 462 819
Size: 263 84 115 357
Fragments arranged by size:
357 263 115 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 108 210 389 819
Size: 108 102 179 430
Fragments arranged by size:
430 179 108 102
DraIII CAC_nnn'GTG
Cuts at: 0 729 819
Size: 729 90
EaeI y'GGCC_r
Cuts at: 0 344 714 819
Size: 344 370 105
Fragments arranged by size:
370 344 105
EarI CTCTTCn'nnn_
Cuts at: 0 382 700 819
Size: 382 318 119
Fragments arranged by size:
382 318 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 819
Size: 211 608
EcoICRI GAG'CTC
Cuts at: 0 686 819
Size: 686 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 37 275 363 580 799 819
Size: 25 12 238 88 217 219 20
Fragments arranged by size:
238 219 217 88 25 20 12
EcoO109I rG'GnC_Cy
Cuts at: 0 93 819
Size: 93 726
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 819
Size: 237 582
HaeII r_GCGC'y
Cuts at: 0 164 665 819
Size: 164 501 154
Fragments arranged by size:
501 164 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 428 460 604 636 819
Size: 428 32 144 32 183
Fragments arranged by size:
428 183 144 32 32
KasI G'GCGC_C
Cuts at: 0 160 661 819
Size: 160 501 158
Fragments arranged by size:
501 160 158
KpnI G_GTAC'C
Cuts at: 0 387 819
Size: 387 432
MluI A'CGCG_T
Cuts at: 0 72 314 819
Size: 72 242 505
Fragments arranged by size:
505 242 72
MscI TGG'CCA
Cuts at: 0 346 819
Size: 346 473
MslI CAynn'nnrTG
Cuts at: 0 286 398 461 819
Size: 286 112 63 358
Fragments arranged by size:
358 286 112 63
MspA1I CmG'CkG
Cuts at: 0 265 745 819
Size: 265 480 74
Fragments arranged by size:
480 265 74
NarI GG'CG_CC
Cuts at: 0 161 662 819
Size: 161 501 157
Fragments arranged by size:
501 161 157
NcoI C'CATG_G
Cuts at: 0 462 819
Size: 462 357
NsiI A_TGCA'T
Cuts at: 0 63 819
Size: 63 756
NspI r_CATG'y
Cuts at: 0 644 819
Size: 644 175
PasI CC'CwG_GG
Cuts at: 0 495 819
Size: 495 324
PmlI CAC'GTG
Cuts at: 0 396 819
Size: 396 423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 819
Size: 62 32 725
Fragments arranged by size:
725 62 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 819
Size: 93 726
PvuII CAG'CTG
Cuts at: 0 745 819
Size: 745 74
SacI G_AGCT'C
Cuts at: 0 688 819
Size: 688 131
SacII CC_GC'GG
Cuts at: 0 266 819
Size: 266 553
SapI GCTCTTCn'nnn_
Cuts at: 0 382 819
Size: 382 437
SfcI C'TryA_G
Cuts at: 0 471 819
Size: 471 348
SfoI GGC'GCC
Cuts at: 0 162 663 819
Size: 162 501 156
Fragments arranged by size:
501 162 156
SmaI CCC'GGG
Cuts at: 0 83 819
Size: 83 736
SmlI C'TyrA_G
Cuts at: 0 88 819
Size: 88 731
StyI C'CwwG_G
Cuts at: 0 462 819
Size: 462 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 388 598 705 819
Size: 177 211 210 107 114
Fragments arranged by size:
211 210 177 114 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 594 626 819
Size: 594 32 193
Fragments arranged by size:
594 193 32
Tth111I GACn'n_nGTC
Cuts at: 0 148 819
Size: 148 671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 129 288 730 819
Size: 129 159 442 89
Fragments arranged by size:
442 159 129 89
XmaI C'CCGG_G
Cuts at: 0 81 819
Size: 81 738
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AleI AloI AlwNI
AvaI BanI BanII BbeI BbsI BclI BfrBI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I KasI KpnI MluI MscI MslI MspA1I
NarI NcoI NsiI NspI PasI PmlI PpiI PpuMI
PvuII SacI SacII SapI SfcI SfoI SmaI SmlI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AlfI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaI BsaBI BseRI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI MfeI MmeI NaeI
NdeI NgoMIV NheI NotI NruI PacI PciI PflMI
PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmnI ZraI