Restriction map for
pF1KB6382
(Linear) (Six-base) MAPSORT of: KIBB6382.seq Check: 6958 from: 1 to: 900
KIBB6382.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 477 900
Size: 184 293 423
Fragments arranged by size:
423 293 184
AflIII A'CryG_T
Cuts at: 0 734 790 900
Size: 734 56 110
Fragments arranged by size:
734 110 56
ApoI r'AATT_y
Cuts at: 0 184 900
Size: 184 716
AvaI C'yCGr_G
Cuts at: 0 707 900
Size: 707 193
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 889 900
Size: 483 406 11
Fragments arranged by size:
483 406 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 262 900
Size: 262 638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 640 900
Size: 31 609 260
Fragments arranged by size:
609 260 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 805 900
Size: 34 771 95
Fragments arranged by size:
771 95 34
BsaI GGTCTCn'nnnn_
Cuts at: 0 442 900
Size: 442 458
BsaAI yAC'GTr
Cuts at: 0 793 900
Size: 793 107
BsaHI Gr'CG_yC
Cuts at: 0 638 900
Size: 638 262
BsaWI w'CCGG_w
Cuts at: 0 81 900
Size: 81 819
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 605 900
Size: 49 556 295
Fragments arranged by size:
556 295 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 900
Size: 736 164
BsiEI CG_ry'CG
Cuts at: 0 68 900
Size: 68 832
BsiHKAI G_wGCw'C
Cuts at: 0 708 772 900
Size: 708 64 128
Fragments arranged by size:
708 128 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 900
Size: 31 869
Bsp1286I G_dGCh'C
Cuts at: 0 708 772 900
Size: 708 64 128
Fragments arranged by size:
708 128 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 758 900
Size: 758 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 211 900
Size: 211 689
DraIII CAC_nnn'GTG
Cuts at: 0 211 900
Size: 211 689
DrdI GACnn_nn'nnGTC
Cuts at: 0 523 900
Size: 523 377
EaeI y'GGCC_r
Cuts at: 0 65 900
Size: 65 835
EagI C'GGCC_G
Cuts at: 0 65 900
Size: 65 835
EarI CTCTTCn'nnn_
Cuts at: 0 392 900
Size: 392 508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 184 477 640 900
Size: 31 153 293 163 260
Fragments arranged by size:
293 260 163 153 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 900
Size: 10 890
EcoO109I rG'GnC_Cy
Cuts at: 0 120 591 900
Size: 120 471 309
Fragments arranged by size:
471 309 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 900
Size: 260 32 608
Fragments arranged by size:
608 260 32
MslI CAynn'nnrTG
Cuts at: 0 695 793 900
Size: 695 98 107
Fragments arranged by size:
695 107 98
MspA1I CmG'CkG
Cuts at: 0 643 900
Size: 643 257
NotI GC'GGCC_GC
Cuts at: 0 65 900
Size: 65 835
NspI r_CATG'y
Cuts at: 0 345 900
Size: 345 555
PfoI T'CCnGG_A
Cuts at: 0 740 900
Size: 740 160
PpuMI rG'GwC_Cy
Cuts at: 0 120 591 900
Size: 120 471 309
Fragments arranged by size:
471 309 120
SmlI C'TyrA_G
Cuts at: 0 49 707 820 900
Size: 49 658 113 80
Fragments arranged by size:
658 113 80 49
StuI AGG'CCT
Cuts at: 0 778 900
Size: 778 122
StyI C'CwwG_G
Cuts at: 0 314 900
Size: 314 586
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 900
Size: 637 263
Tth111I GACn'n_nGTC
Cuts at: 0 478 900
Size: 478 422
XhoI C'TCGA_G
Cuts at: 0 707 900
Size: 707 193
XmnI GAAnn'nnTTC
Cuts at: 0 302 900
Size: 302 598
Enzymes that do cut:
AcuI AflIII ApoI AvaI BbsI BciVI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BstAPI DraIII DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I Hin4I MslI MspA1I NotI
NspI PfoI PpuMI SmlI StuI StyI TaqII Tth111I
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaXI BseYI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI XbaI XcmI
XmaI ZraI