Restriction map for
pF1KB6374
(Linear) (Six-base) MAPSORT of: KIBB6374.seq Check: 5084 from: 1 to: 828
KIBB6374.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 263 286 828
Size: 48 215 23 542
Fragments arranged by size:
542 215 48 23
AflIII A'CryG_T
Cuts at: 0 20 828
Size: 20 808
ApaI G_GGCC'C
Cuts at: 0 41 50 828
Size: 41 9 778
Fragments arranged by size:
778 41 9
ApaLI G'TGCA_C
Cuts at: 0 682 828
Size: 682 146
ApoI r'AATT_y
Cuts at: 0 132 828
Size: 132 696
AvaI C'yCGr_G
Cuts at: 0 33 42 828
Size: 33 9 786
Fragments arranged by size:
786 33 9
BanII G_rGCy'C
Cuts at: 0 41 50 828
Size: 41 9 778
Fragments arranged by size:
778 41 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 766 828
Size: 766 62
BglI GCCn_nnn'nGGC
Cuts at: 0 45 828
Size: 45 783
Bme1580I G_kGCm'C
Cuts at: 0 41 50 686 828
Size: 41 9 636 142
Fragments arranged by size:
636 142 41 9
BmgBI CAC'GTC
Cuts at: 0 83 687 828
Size: 83 604 141
Fragments arranged by size:
604 141 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 828
Size: 592 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 828
Size: 150 678
BsaI GGTCTCn'nnnn_
Cuts at: 0 462 828
Size: 462 366
BsaBI GATnn'nnATC
Cuts at: 0 593 754 828
Size: 593 161 74
Fragments arranged by size:
593 161 74
BsaWI w'CCGG_w
Cuts at: 0 26 828
Size: 26 802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 490 828
Size: 460 30 338
Fragments arranged by size:
460 338 30
BseYI C'CCAG_C
Cuts at: 0 50 828
Size: 50 778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 379 828
Size: 105 274 449
Fragments arranged by size:
449 274 105
BsiHKAI G_wGCw'C
Cuts at: 0 686 828
Size: 686 142
Bsp1286I G_dGCh'C
Cuts at: 0 41 50 686 828
Size: 41 9 636 142
Fragments arranged by size:
636 142 41 9
BspHI T'CATG_A
Cuts at: 0 317 828
Size: 317 511
BstYI r'GATC_y
Cuts at: 0 412 828
Size: 412 416
BtsI GCAGTG_nn'
Cuts at: 0 116 828
Size: 116 712
DraI TTT'AAA
Cuts at: 0 606 828
Size: 606 222
EaeI y'GGCC_r
Cuts at: 0 512 614 828
Size: 512 102 214
Fragments arranged by size:
512 214 102
EarI CTCTTCn'nnn_
Cuts at: 0 425 828
Size: 425 403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 263 286 592 828
Size: 48 215 23 306 236
Fragments arranged by size:
306 236 215 48 23
EcoO109I rG'GnC_Cy
Cuts at: 0 37 38 46 47 254 828
Size: 37 1 8 1 207 574
Fragments arranged by size:
574 207 37 8 1 1
HaeII r_GCGC'y
Cuts at: 0 291 828
Size: 291 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 788 820 828
Size: 788 32 8
Fragments arranged by size:
788 32 8
MscI TGG'CCA
Cuts at: 0 514 616 828
Size: 514 102 212
Fragments arranged by size:
514 212 102
MspA1I CmG'CkG
Cuts at: 0 89 102 453 570 828
Size: 89 13 351 117 258
Fragments arranged by size:
351 258 117 89 13
NspI r_CATG'y
Cuts at: 0 24 828
Size: 24 804
PciI A'CATG_T
Cuts at: 0 20 828
Size: 20 808
PfoI T'CCnGG_A
Cuts at: 0 56 828
Size: 56 772
PpuMI rG'GwC_Cy
Cuts at: 0 254 828
Size: 254 574
PsiI TTA'TAA
Cuts at: 0 818 828
Size: 818 10
PspOMI G'GGCC_C
Cuts at: 0 37 46 828
Size: 37 9 782
Fragments arranged by size:
782 37 9
PstI C_TGCA'G
Cuts at: 0 336 429 828
Size: 336 93 399
Fragments arranged by size:
399 336 93
PvuII CAG'CTG
Cuts at: 0 453 570 828
Size: 453 117 258
Fragments arranged by size:
453 258 117
SfcI C'TryA_G
Cuts at: 0 175 332 425 828
Size: 175 157 93 403
Fragments arranged by size:
403 175 157 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 45 828
Size: 45 783
SmaI CCC'GGG
Cuts at: 0 35 828
Size: 35 793
SmlI C'TyrA_G
Cuts at: 0 165 828
Size: 165 663
StyI C'CwwG_G
Cuts at: 0 221 828
Size: 221 607
XmaI C'CCGG_G
Cuts at: 0 33 828
Size: 33 795
XmnI GAAnn'nnTTC
Cuts at: 0 325 828
Size: 325 503
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI AvaI BanII BciVI
BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BstYI BtsI
DraI EaeI EarI Eco57MI EcoO109I HaeII Hin4I MscI
MspA1I NspI PciI PfoI PpuMI PsiI PspOMI PstI
PvuII SfcI SfiI SmaI SmlI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BsaAI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI ZraI