Restriction map for
pF1KB6367
(Linear) (Six-base) MAPSORT of: KIBB6367.seq Check: 4664 from: 1 to: 807
KIBB6367.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 807
Size: 165 642
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 807
Size: 49 32 726
Fragments arranged by size:
726 49 32
AlwNI CAG_nnn'CTG
Cuts at: 0 114 807
Size: 114 693
ApaLI G'TGCA_C
Cuts at: 0 340 807
Size: 340 467
ApoI r'AATT_y
Cuts at: 0 679 807
Size: 679 128
BanII G_rGCy'C
Cuts at: 0 20 807
Size: 20 787
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 735 794 807
Size: 639 96 59 13
Fragments arranged by size:
639 96 59 13
BbvCI CC'TCA_GC
Cuts at: 0 220 807
Size: 220 587
Bme1580I G_kGCm'C
Cuts at: 0 344 807
Size: 344 463
BmrI ACTGGGnnnn_n'
Cuts at: 0 687 807
Size: 687 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 613 807
Size: 424 189 194
Fragments arranged by size:
424 194 189
Bpu10I CC'TnA_GC
Cuts at: 0 14 220 268 807
Size: 14 206 48 539
Fragments arranged by size:
539 206 48 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 707 807
Size: 517 190 100
Fragments arranged by size:
517 190 100
BsaBI GATnn'nnATC
Cuts at: 0 661 807
Size: 661 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 807
Size: 354 453
BsiHKAI G_wGCw'C
Cuts at: 0 20 103 274 344 807
Size: 20 83 171 70 463
Fragments arranged by size:
463 171 83 70 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 103 274 344 807
Size: 20 83 171 70 463
Fragments arranged by size:
463 171 83 70 20
BsrDI GCAATG_nn'
Cuts at: 0 57 807
Size: 57 750
BsrGI T'GTAC_A
Cuts at: 0 486 807
Size: 486 321
BstYI r'GATC_y
Cuts at: 0 603 807
Size: 603 204
BtgI C'CryG_G
Cuts at: 0 209 807
Size: 209 598
EaeI y'GGCC_r
Cuts at: 0 206 765 807
Size: 206 559 42
Fragments arranged by size:
559 206 42
EcoICRI GAG'CTC
Cuts at: 0 18 807
Size: 18 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 424 613 807
Size: 165 259 189 194
Fragments arranged by size:
259 194 189 165
EcoO109I rG'GnC_Cy
Cuts at: 0 111 807
Size: 111 696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 807
Size: 120 687
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 588 620 807
Size: 396 32 160 32 187
Fragments arranged by size:
396 187 160 32 32
HindIII A'AGCT_T
Cuts at: 0 396 807
Size: 396 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 807
Size: 182 625
MscI TGG'CCA
Cuts at: 0 208 807
Size: 208 599
MspA1I CmG'CkG
Cuts at: 0 591 807
Size: 591 216
NcoI C'CATG_G
Cuts at: 0 209 807
Size: 209 598
NdeI CA'TA_TG
Cuts at: 0 417 807
Size: 417 390
NspI r_CATG'y
Cuts at: 0 738 807
Size: 738 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 807
Size: 114 693
PfoI T'CCnGG_A
Cuts at: 0 107 254 377 807
Size: 107 147 123 430
Fragments arranged by size:
430 147 123 107
PpuMI rG'GwC_Cy
Cuts at: 0 111 807
Size: 111 696
PvuII CAG'CTG
Cuts at: 0 591 807
Size: 591 216
SacI G_AGCT'C
Cuts at: 0 20 807
Size: 20 787
SfcI C'TryA_G
Cuts at: 0 168 586 807
Size: 168 418 221
Fragments arranged by size:
418 221 168
SmlI C'TyrA_G
Cuts at: 0 532 722 807
Size: 532 190 85
Fragments arranged by size:
532 190 85
StyI C'CwwG_G
Cuts at: 0 209 524 807
Size: 209 315 283
Fragments arranged by size:
315 283 209
TatI w'GTAC_w
Cuts at: 0 486 807
Size: 486 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 236 264 268 296 807
Size: 236 28 4 28 511
Fragments arranged by size:
511 236 28 28 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 588 807
Size: 30 558 219
Fragments arranged by size:
558 219 30
Enzymes that do cut:
AcuI AloI AlwNI ApaLI ApoI BanII BbsI BbvCI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsgI BsiHKAI
Bsp1286I BsrDI BsrGI BstYI BtgI EaeI EcoICRI Eco57MI
EcoO109I FalI Hin4I HindIII MmeI MscI MspA1I NcoI
NdeI NspI PflMI PfoI PpuMI PvuII SacI SfcI
SmlI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI