Restriction map for
pF1KB6356
(Linear) (Six-base) MAPSORT of: KIBB6356.seq Check: 3753 from: 1 to: 885
KIBB6356.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 68 123 885
Size: 68 55 762
Fragments arranged by size:
762 68 55
AclI AA'CG_TT
Cuts at: 0 286 885
Size: 286 599
AflIII A'CryG_T
Cuts at: 0 836 885
Size: 836 49
AlwNI CAG_nnn'CTG
Cuts at: 0 345 485 885
Size: 345 140 400
Fragments arranged by size:
400 345 140
ApaI G_GGCC'C
Cuts at: 0 98 629 869 885
Size: 98 531 240 16
Fragments arranged by size:
531 240 98 16
ApoI r'AATT_y
Cuts at: 0 229 885
Size: 229 656
AvaI C'yCGr_G
Cuts at: 0 97 430 677 885
Size: 97 333 247 208
Fragments arranged by size:
333 247 208 97
AvrII C'CTAG_G
Cuts at: 0 291 885
Size: 291 594
BanI G'GyrC_C
Cuts at: 0 68 105 123 213 394 529 885
Size: 68 37 18 90 181 135 356
Fragments arranged by size:
356 181 135 90 68 37 18
BanII G_rGCy'C
Cuts at: 0 98 629 869 885
Size: 98 531 240 16
Fragments arranged by size:
531 240 98 16
BbeI G_GCGC'C
Cuts at: 0 109 398 885
Size: 109 289 487
Fragments arranged by size:
487 289 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 679 885
Size: 679 206
BclI T'GATC_A
Cuts at: 0 246 885
Size: 246 639
BglI GCCn_nnn'nGGC
Cuts at: 0 494 885
Size: 494 391
BlpI GC'TnA_GC
Cuts at: 0 272 885
Size: 272 613
Bme1580I G_kGCm'C
Cuts at: 0 98 116 629 869 885
Size: 98 18 513 240 16
Fragments arranged by size:
513 240 98 18 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 757 885
Size: 757 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 885
Size: 484 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 885
Size: 295 590
BsaAI yAC'GTr
Cuts at: 0 546 885
Size: 546 339
BsaBI GATnn'nnATC
Cuts at: 0 596 885
Size: 596 289
BsaHI Gr'CG_yC
Cuts at: 0 106 395 885
Size: 106 289 490
Fragments arranged by size:
490 289 106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 869 885
Size: 376 493 16
Fragments arranged by size:
493 376 16
BseYI C'CCAG_C
Cuts at: 0 132 296 810 862 885
Size: 132 164 514 52 23
Fragments arranged by size:
514 164 132 52 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 451 885
Size: 259 192 434
Fragments arranged by size:
434 259 192
BsiEI CG_ry'CG
Cuts at: 0 359 406 600 885
Size: 359 47 194 285
Fragments arranged by size:
359 285 194 47
Bsp1286I G_dGCh'C
Cuts at: 0 98 116 629 869 885
Size: 98 18 513 240 16
Fragments arranged by size:
513 240 98 18 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 885
Size: 135 750
BsrFI r'CCGG_y
Cuts at: 0 78 401 885
Size: 78 323 484
Fragments arranged by size:
484 323 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 374 485 885
Size: 374 111 400
Fragments arranged by size:
400 374 111
BtgI C'CryG_G
Cuts at: 0 170 885
Size: 170 715
BtsI GCAGTG_nn'
Cuts at: 0 202 699 721 885
Size: 202 497 22 164
Fragments arranged by size:
497 202 164 22
EaeI y'GGCC_r
Cuts at: 0 356 403 422 540 885
Size: 356 47 19 118 345
Fragments arranged by size:
356 345 118 47 19
EagI C'GGCC_G
Cuts at: 0 356 403 885
Size: 356 47 482
Fragments arranged by size:
482 356 47
EarI CTCTTCn'nnn_
Cuts at: 0 157 885
Size: 157 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 448 885
Size: 406 42 437
Fragments arranged by size:
437 406 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 885
Size: 484 401
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 885
Size: 349 536
EcoO109I rG'GnC_Cy
Cuts at: 0 625 626 723 866 885
Size: 625 1 97 143 19
Fragments arranged by size:
625 143 97 19 1
EcoRI G'AATT_C
Cuts at: 0 229 885
Size: 229 656
HaeII r_GCGC'y
Cuts at: 0 85 109 398 863 885
Size: 85 24 289 465 22
Fragments arranged by size:
465 289 85 24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 294 326 546 578 885
Size: 294 32 220 32 307
Fragments arranged by size:
307 294 220 32 32
KasI G'GCGC_C
Cuts at: 0 105 394 885
Size: 105 289 491
Fragments arranged by size:
491 289 105
KpnI G_GTAC'C
Cuts at: 0 72 127 885
Size: 72 55 758
Fragments arranged by size:
758 72 55
MscI TGG'CCA
Cuts at: 0 542 885
Size: 542 343
MspA1I CmG'CkG
Cuts at: 0 408 465 482 777 885
Size: 408 57 17 295 108
Fragments arranged by size:
408 295 108 57 17
NaeI GCC'GGC
Cuts at: 0 80 403 885
Size: 80 323 482
Fragments arranged by size:
482 323 80
NarI GG'CG_CC
Cuts at: 0 106 395 885
Size: 106 289 490
Fragments arranged by size:
490 289 106
NgoMIV G'CCGG_C
Cuts at: 0 78 401 885
Size: 78 323 484
Fragments arranged by size:
484 323 78
NspI r_CATG'y
Cuts at: 0 840 885
Size: 840 45
PasI CC'CwG_GG
Cuts at: 0 64 345 885
Size: 64 281 540
Fragments arranged by size:
540 281 64
PciI A'CATG_T
Cuts at: 0 836 885
Size: 836 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 885
Size: 507 378
PfoI T'CCnGG_A
Cuts at: 0 500 587 885
Size: 500 87 298
Fragments arranged by size:
500 298 87
PmlI CAC'GTG
Cuts at: 0 546 885
Size: 546 339
PspOMI G'GGCC_C
Cuts at: 0 94 625 865 885
Size: 94 531 240 20
Fragments arranged by size:
531 240 94 20
PstI C_TGCA'G
Cuts at: 0 481 885
Size: 481 404
PvuI CG_AT'CG
Cuts at: 0 600 885
Size: 600 285
PvuII CAG'CTG
Cuts at: 0 482 885
Size: 482 403
SfcI C'TryA_G
Cuts at: 0 477 550 766 885
Size: 477 73 216 119
Fragments arranged by size:
477 216 119 73
SfoI GGC'GCC
Cuts at: 0 107 396 885
Size: 107 289 489
Fragments arranged by size:
489 289 107
SmaI CCC'GGG
Cuts at: 0 99 432 885
Size: 99 333 453
Fragments arranged by size:
453 333 99
SrfI GCCC'GGGC
Cuts at: 0 99 885
Size: 99 786
StyI C'CwwG_G
Cuts at: 0 176 291 885
Size: 176 115 594
Fragments arranged by size:
594 176 115
TatI w'GTAC_w
Cuts at: 0 513 885
Size: 513 372
XmaI C'CCGG_G
Cuts at: 0 97 430 885
Size: 97 333 455
Fragments arranged by size:
455 333 97
Enzymes that do cut:
Acc65I AclI AflIII AlwNI ApaI ApoI AvaI AvrII
BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I
BplI BpmI BsaI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrFI BstAPI BtgI BtsI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI KpnI MscI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PspOMI
PstI PvuI PvuII SfcI SfoI SmaI SrfI StyI
TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BfrBI BglII BmgBI BmrI
BmtI Bpu10I BpuEI BsaWI BsaXI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MslI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI