Restriction map for
pF1KB6352
(Linear) (Six-base) MAPSORT of: KIBB6352.seq Check: 3844 from: 1 to: 879
KIBB6352.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 879
Size: 367 512
AlwNI CAG_nnn'CTG
Cuts at: 0 214 879
Size: 214 665
AvaI C'yCGr_G
Cuts at: 0 491 879
Size: 491 388
BamHI G'GATC_C
Cuts at: 0 524 879
Size: 524 355
BanI G'GyrC_C
Cuts at: 0 620 879
Size: 620 259
BanII G_rGCy'C
Cuts at: 0 50 879
Size: 50 829
BbeI G_GCGC'C
Cuts at: 0 624 879
Size: 624 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 124 340 800 879
Size: 124 216 460 79
Fragments arranged by size:
460 216 124 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 722 879
Size: 722 157
BclI T'GATC_A
Cuts at: 0 59 879
Size: 59 820
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 879
Size: 636 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 879
Size: 494 385
Bpu10I CC'TnA_GC
Cuts at: 0 613 879
Size: 613 266
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 879
Size: 310 569
BsaAI yAC'GTr
Cuts at: 0 462 849 879
Size: 462 387 30
Fragments arranged by size:
462 387 30
BsaHI Gr'CG_yC
Cuts at: 0 621 879
Size: 621 258
BsaWI w'CCGG_w
Cuts at: 0 164 879
Size: 164 715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 467 476 497 879
Size: 446 21 9 21 382
Fragments arranged by size:
446 382 21 21 9
BsiHKAI G_wGCw'C
Cuts at: 0 50 879
Size: 50 829
BsmBI CGTCTCn'nnnn_
Cuts at: 0 655 879
Size: 655 224
Bsp1286I G_dGCh'C
Cuts at: 0 50 879
Size: 50 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 879
Size: 733 146
BsrBI CCG'CTC
Cuts at: 0 129 879
Size: 129 750
BsrDI GCAATG_nn'
Cuts at: 0 26 879
Size: 26 853
BsrFI r'CCGG_y
Cuts at: 0 301 617 879
Size: 301 316 262
Fragments arranged by size:
316 301 262
BssSI C'ACGA_G
Cuts at: 0 7 697 879
Size: 7 690 182
Fragments arranged by size:
690 182 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 879
Size: 699 180
BstYI r'GATC_y
Cuts at: 0 524 879
Size: 524 355
Bsu36I CC'TnA_GG
Cuts at: 0 450 671 879
Size: 450 221 208
Fragments arranged by size:
450 221 208
BtgI C'CryG_G
Cuts at: 0 503 879
Size: 503 376
BtsI GCAGTG_nn'
Cuts at: 0 526 879
Size: 526 353
ClaI AT'CG_AT
Cuts at: 0 584 879
Size: 584 295
DrdI GACnn_nn'nnGTC
Cuts at: 0 781 879
Size: 781 98
EaeI y'GGCC_r
Cuts at: 0 290 377 464 530 879
Size: 290 87 87 66 349
Fragments arranged by size:
349 290 87 87 66
EarI CTCTTCn'nnn_
Cuts at: 0 71 269 879
Size: 71 198 610
Fragments arranged by size:
610 198 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 879
Size: 297 582
EcoICRI GAG'CTC
Cuts at: 0 48 879
Size: 48 831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 879
Size: 494 385
EcoNI CCTnn'n_nnAGG
Cuts at: 0 449 532 879
Size: 449 83 347
Fragments arranged by size:
449 347 83
FspI TGC'GCA
Cuts at: 0 343 879
Size: 343 536
HaeII r_GCGC'y
Cuts at: 0 624 879
Size: 624 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 763 795 879
Size: 288 32 443 32 84
Fragments arranged by size:
443 288 84 32 32
KasI G'GCGC_C
Cuts at: 0 620 879
Size: 620 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 879
Size: 251 628
MscI TGG'CCA
Cuts at: 0 466 879
Size: 466 413
MspA1I CmG'CkG
Cuts at: 0 57 636 762 879
Size: 57 579 126 117
Fragments arranged by size:
579 126 117 57
NaeI GCC'GGC
Cuts at: 0 619 879
Size: 619 260
NarI GG'CG_CC
Cuts at: 0 621 879
Size: 621 258
NgoMIV G'CCGG_C
Cuts at: 0 617 879
Size: 617 262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 879
Size: 664 32 183
Fragments arranged by size:
664 183 32
PstI C_TGCA'G
Cuts at: 0 371 879
Size: 371 508
PvuII CAG'CTG
Cuts at: 0 57 879
Size: 57 822
SacI G_AGCT'C
Cuts at: 0 50 879
Size: 50 829
SapI GCTCTTCn'nnn_
Cuts at: 0 71 879
Size: 71 808
SfcI C'TryA_G
Cuts at: 0 367 879
Size: 367 512
SfoI GGC'GCC
Cuts at: 0 622 879
Size: 622 257
SmaI CCC'GGG
Cuts at: 0 493 879
Size: 493 386
StuI AGG'CCT
Cuts at: 0 676 879
Size: 676 203
StyI C'CwwG_G
Cuts at: 0 197 879
Size: 197 682
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 879
Size: 771 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 396 428 446 467 478 499 505 537
Size: 396 32 18 21 11 21 6 32
Cuts at: 537 879
Size: 342
Fragments arranged by size:
396 342 32 32 21 21 18 11
6
XmaI C'CCGG_G
Cuts at: 0 491 879
Size: 491 388
Enzymes that do cut:
AlfI AlwNI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtsI ClaI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI FspI
HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI
NgoMIV PpiI PstI PvuII SacI SapI SfcI SfoI
SmaI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaBI BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI
CspCI DraI DraIII EagI EcoO109I EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI