Restriction map for
pF1KB6350
(Linear) (Six-base) MAPSORT of: KIBB6350.seq Check: 3984 from: 1 to: 879
KIBB6350.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 216 249 879
Size: 216 33 630
Fragments arranged by size:
630 216 33
BanI G'GyrC_C
Cuts at: 0 81 574 879
Size: 81 493 305
Fragments arranged by size:
493 305 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 879
Size: 553 326
BbvCI CC'TCA_GC
Cuts at: 0 358 428 879
Size: 358 70 451
Fragments arranged by size:
451 358 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 84 879
Size: 84 795
BglII A'GATC_T
Cuts at: 0 367 879
Size: 367 512
BmrI ACTGGGnnnn_n'
Cuts at: 0 61 879
Size: 61 818
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 318 693 879
Size: 318 375 186
Fragments arranged by size:
375 318 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 368 688 879
Size: 288 80 320 191
Fragments arranged by size:
320 288 191 80
Bpu10I CC'TnA_GC
Cuts at: 0 358 428 626 879
Size: 358 70 198 253
Fragments arranged by size:
358 253 198 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 289 879
Size: 289 590
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 263 879
Size: 233 30 616
Fragments arranged by size:
616 233 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 365 375 489 847 879
Size: 365 10 114 358 32
Fragments arranged by size:
365 358 114 32 10
BsiHKAI G_wGCw'C
Cuts at: 0 632 879
Size: 632 247
Bsp1286I G_dGCh'C
Cuts at: 0 632 879
Size: 632 247
BspHI T'CATG_A
Cuts at: 0 558 879
Size: 558 321
BsrDI GCAATG_nn'
Cuts at: 0 155 395 656 879
Size: 155 240 261 223
Fragments arranged by size:
261 240 223 155
BssSI C'ACGA_G
Cuts at: 0 471 879
Size: 471 408
BstAPI GCAn_nnn'nTGC
Cuts at: 0 166 879
Size: 166 713
BstYI r'GATC_y
Cuts at: 0 367 879
Size: 367 512
BtgI C'CryG_G
Cuts at: 0 126 879
Size: 126 753
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 879
Size: 19 860
BtsI GCAGTG_nn'
Cuts at: 0 446 879
Size: 446 433
DrdI GACnn_nn'nnGTC
Cuts at: 0 457 879
Size: 457 422
EarI CTCTTCn'nnn_
Cuts at: 0 140 274 632 879
Size: 140 134 358 247
Fragments arranged by size:
358 247 140 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 368 688 879
Size: 288 80 320 191
Fragments arranged by size:
320 288 191 80
EcoO109I rG'GnC_Cy
Cuts at: 0 414 879
Size: 414 465
EcoRV GAT'ATC
Cuts at: 0 701 879
Size: 701 178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 400 433 570 805 879
Size: 400 33 137 235 74
Fragments arranged by size:
400 235 137 74 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 879
Size: 685 32 162
Fragments arranged by size:
685 162 32
HindIII A'AGCT_T
Cuts at: 0 209 879
Size: 209 670
MslI CAynn'nnrTG
Cuts at: 0 250 557 879
Size: 250 307 322
Fragments arranged by size:
322 307 250
NcoI C'CATG_G
Cuts at: 0 126 879
Size: 126 753
PfoI T'CCnGG_A
Cuts at: 0 345 879
Size: 345 534
PpuMI rG'GwC_Cy
Cuts at: 0 414 879
Size: 414 465
PstI C_TGCA'G
Cuts at: 0 375 879
Size: 375 504
SfcI C'TryA_G
Cuts at: 0 371 879
Size: 371 508
StyI C'CwwG_G
Cuts at: 0 126 879
Size: 126 753
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 879
Size: 306 573
TatI w'GTAC_w
Cuts at: 0 770 879
Size: 770 109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 879
Size: 306 573
Enzymes that do cut:
BaeI BanI BbsI BbvCI BciVI BglII BmrI BplI
BpmI Bpu10I BsaI BsaXI BseRI BsiHKAI Bsp1286I BspHI
BsrDI BssSI BstAPI BstYI BtgI BtgZI BtsI DrdI
EarI Eco57MI EcoO109I EcoRV FalI Hin4I HindIII MslI
NcoI PfoI PpuMI PstI SfcI StyI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanII BbeI BcgI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI