Restriction map for
pF1KB6338
(Linear) (Six-base) MAPSORT of: KIBB6338.seq Check: 8218 from: 1 to: 870
KIBB6338.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 350 870
Size: 350 520
Acc65I G'GTAC_C
Cuts at: 0 725 870
Size: 725 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 262 638 870
Size: 188 74 376 232
Fragments arranged by size:
376 232 188 74
AleI CACnn'nnGTG
Cuts at: 0 96 870
Size: 96 774
AlwNI CAG_nnn'CTG
Cuts at: 0 661 870
Size: 661 209
ApoI r'AATT_y
Cuts at: 0 838 870
Size: 838 32
AvaI C'yCGr_G
Cuts at: 0 220 321 870
Size: 220 101 549
Fragments arranged by size:
549 220 101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 709 742 870
Size: 709 33 128
Fragments arranged by size:
709 128 33
BanI G'GyrC_C
Cuts at: 0 371 725 870
Size: 371 354 145
Fragments arranged by size:
371 354 145
BanII G_rGCy'C
Cuts at: 0 68 870
Size: 68 802
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 644 802 870
Size: 476 168 158 68
Fragments arranged by size:
476 168 158 68
BglII A'GATC_T
Cuts at: 0 171 870
Size: 171 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 217 424 870
Size: 79 138 207 446
Fragments arranged by size:
446 207 138 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 665 845 870
Size: 294 371 180 25
Fragments arranged by size:
371 294 180 25
BsaWI w'CCGG_w
Cuts at: 0 68 870
Size: 68 802
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 595 625 870
Size: 595 30 245
Fragments arranged by size:
595 245 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 870
Size: 112 758
BseYI C'CCAG_C
Cuts at: 0 13 870
Size: 13 857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 870
Size: 427 443
BsiHKAI G_wGCw'C
Cuts at: 0 221 870
Size: 221 649
Bsp1286I G_dGCh'C
Cuts at: 0 68 221 870
Size: 68 153 649
Fragments arranged by size:
649 153 68
BspEI T'CCGG_A
Cuts at: 0 68 870
Size: 68 802
BstBI TT'CG_AA
Cuts at: 0 807 870
Size: 807 63
BstYI r'GATC_y
Cuts at: 0 171 870
Size: 171 699
Bsu36I CC'TnA_GG
Cuts at: 0 266 484 870
Size: 266 218 386
Fragments arranged by size:
386 266 218
BtsI GCAGTG_nn'
Cuts at: 0 506 698 773 870
Size: 506 192 75 97
Fragments arranged by size:
506 192 97 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 167 202 870
Size: 167 35 668
Fragments arranged by size:
668 167 35
DraI TTT'AAA
Cuts at: 0 843 870
Size: 843 27
DraIII CAC_nnn'GTG
Cuts at: 0 284 870
Size: 284 586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 188 217 262 424 638 870
Size: 79 109 29 45 162 214 232
Fragments arranged by size:
232 214 162 109 79 45 29
EcoO109I rG'GnC_Cy
Cuts at: 0 444 855 870
Size: 444 411 15
Fragments arranged by size:
444 411 15
EcoRV GAT'ATC
Cuts at: 0 74 870
Size: 74 796
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 595 627 870
Size: 156 32 407 32 243
Fragments arranged by size:
407 243 156 32 32
HincII GTy'rAC
Cuts at: 0 351 698 870
Size: 351 347 172
Fragments arranged by size:
351 347 172
KpnI G_GTAC'C
Cuts at: 0 729 870
Size: 729 141
MslI CAynn'nnrTG
Cuts at: 0 96 870
Size: 96 774
MspA1I CmG'CkG
Cuts at: 0 117 154 497 608 870
Size: 117 37 343 111 262
Fragments arranged by size:
343 262 117 111 37
NspI r_CATG'y
Cuts at: 0 162 870
Size: 162 708
PflMI CCAn_nnn'nTGG
Cuts at: 0 284 870
Size: 284 586
PfoI T'CCnGG_A
Cuts at: 0 194 870
Size: 194 676
PpuMI rG'GwC_Cy
Cuts at: 0 855 870
Size: 855 15
PspXI vC'TCGA_Gb
Cuts at: 0 321 870
Size: 321 549
PstI C_TGCA'G
Cuts at: 0 625 870
Size: 625 245
PvuII CAG'CTG
Cuts at: 0 117 154 497 870
Size: 117 37 343 373
Fragments arranged by size:
373 343 117 37
SalI G'TCGA_C
Cuts at: 0 349 870
Size: 349 521
SfcI C'TryA_G
Cuts at: 0 621 870
Size: 621 249
SmlI C'TyrA_G
Cuts at: 0 220 309 321 680 860 870
Size: 220 89 12 359 180 10
Fragments arranged by size:
359 220 180 89 12 10
SphI G_CATG'C
Cuts at: 0 162 870
Size: 162 708
StuI AGG'CCT
Cuts at: 0 319 870
Size: 319 551
SwaI ATTT'AAAT
Cuts at: 0 843 870
Size: 843 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 444 870
Size: 444 426
TatI w'GTAC_w
Cuts at: 0 365 870
Size: 365 505
XhoI C'TCGA_G
Cuts at: 0 220 321 870
Size: 220 101 549
Fragments arranged by size:
549 220 101
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApoI AvaI BaeI
BanI BanII BbsI BglII BpmI BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BstBI BstYI
Bsu36I BtsI CspCI DraI DraIII Eco57MI EcoO109I EcoRV
Hin4I HincII KpnI MslI MspA1I NspI PflMI PfoI
PpuMI PspXI PstI PvuII SalI SfcI SmlI SphI
StuI SwaI TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgI BtgZI ClaI DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI