Restriction map for
pF1KB6335
(Linear) (Six-base) MAPSORT of: KIBB6335.seq Check: 1745 from: 1 to: 783
KIBB6335.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
Acc65I G'GTAC_C
Cuts at: 0 629 783
Size: 629 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 783
Size: 456 327
ApoI r'AATT_y
Cuts at: 0 380 427 502 783
Size: 380 47 75 281
Fragments arranged by size:
380 281 75 47
AscI GG'CGCG_CC
Cuts at: 0 10 783
Size: 10 773
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 613 646 783
Size: 613 33 137
Fragments arranged by size:
613 137 33
BanI G'GyrC_C
Cuts at: 0 629 783
Size: 629 154
BanII G_rGCy'C
Cuts at: 0 28 783
Size: 28 755
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 783
Size: 282 501
BglII A'GATC_T
Cuts at: 0 447 783
Size: 447 336
BmgBI CAC'GTC
Cuts at: 0 132 783
Size: 132 651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 783
Size: 78 705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 40 783
Size: 19 21 743
Fragments arranged by size:
743 21 19
BsaAI yAC'GTr
Cuts at: 0 436 783
Size: 436 347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 455 485 783
Size: 455 30 298
Fragments arranged by size:
455 298 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 783
Size: 586 197
BseYI C'CCAG_C
Cuts at: 0 80 783
Size: 80 703
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 783
Size: 163 620
BsiEI CG_ry'CG
Cuts at: 0 189 783
Size: 189 594
BsmBI CGTCTCn'nnnn_
Cuts at: 0 138 783
Size: 138 645
Bsp1286I G_dGCh'C
Cuts at: 0 28 783
Size: 28 755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 783
Size: 116 667
BsrDI GCAATG_nn'
Cuts at: 0 255 783
Size: 255 528
BsrFI r'CCGG_y
Cuts at: 0 244 783
Size: 244 539
BssHII G'CGCG_C
Cuts at: 0 10 783
Size: 10 773
BstYI r'GATC_y
Cuts at: 0 447 778 783
Size: 447 331 5
Fragments arranged by size:
447 331 5
BtgI C'CryG_G
Cuts at: 0 200 783
Size: 200 583
EaeI y'GGCC_r
Cuts at: 0 197 783
Size: 197 586
EarI CTCTTCn'nnn_
Cuts at: 0 669 783
Size: 669 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 783
Size: 78 705
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 37 783
Size: 19 18 746
Fragments arranged by size:
746 19 18
EcoO109I rG'GnC_Cy
Cuts at: 0 60 783
Size: 60 723
EcoRI G'AATT_C
Cuts at: 0 380 783
Size: 380 403
EcoRV GAT'ATC
Cuts at: 0 258 783
Size: 258 525
HaeII r_GCGC'y
Cuts at: 0 301 783
Size: 301 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 783
Size: 76 32 675
Fragments arranged by size:
675 76 32
KpnI G_GTAC'C
Cuts at: 0 633 783
Size: 633 150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 783
Size: 26 757
MscI TGG'CCA
Cuts at: 0 199 783
Size: 199 584
MslI CAynn'nnrTG
Cuts at: 0 205 220 438 592 783
Size: 205 15 218 154 191
Fragments arranged by size:
218 205 191 154 15
MspA1I CmG'CkG
Cuts at: 0 98 182 401 783
Size: 98 84 219 382
Fragments arranged by size:
382 219 98 84
NaeI GCC'GGC
Cuts at: 0 246 783
Size: 246 537
NcoI C'CATG_G
Cuts at: 0 200 783
Size: 200 583
NgoMIV G'CCGG_C
Cuts at: 0 244 783
Size: 244 539
PpuMI rG'GwC_Cy
Cuts at: 0 60 783
Size: 60 723
PstI C_TGCA'G
Cuts at: 0 113 693 783
Size: 113 580 90
Fragments arranged by size:
580 113 90
PvuII CAG'CTG
Cuts at: 0 401 783
Size: 401 382
SfcI C'TryA_G
Cuts at: 0 109 689 783
Size: 109 580 94
Fragments arranged by size:
580 109 94
SmlI C'TyrA_G
Cuts at: 0 34 55 783
Size: 34 21 728
Fragments arranged by size:
728 34 21
StuI AGG'CCT
Cuts at: 0 76 783
Size: 76 707
StyI C'CwwG_G
Cuts at: 0 200 293 783
Size: 200 93 490
Fragments arranged by size:
490 200 93
TatI w'GTAC_w
Cuts at: 0 395 783
Size: 395 388
XmnI GAAnn'nnTTC
Cuts at: 0 605 783
Size: 605 178
Enzymes that do cut:
Acc65I AlfI ApoI AscI BaeI BanI BanII BciVI
BglII BmgBI BpmI BpuEI BsaAI BsaXI BseRI BseYI
BsgI BsiEI BsmBI Bsp1286I BspMI BsrDI BsrFI BssHII
BstYI BtgI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV PpuMI PstI PvuII SfcI SmlI
StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AseI
AsiSI AvaI AvrII BamHI BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmI
BspEI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI