Restriction map for
pF1KB6333
(Linear) (Six-base) MAPSORT of: KIBB6333.seq Check: 4672 from: 1 to: 783
KIBB6333.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 105 460 631 783
Size: 105 355 171 152
Fragments arranged by size:
355 171 152 105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 337 496 783
Size: 269 68 159 287
Fragments arranged by size:
287 269 159 68
AflIII A'CryG_T
Cuts at: 0 545 783
Size: 545 238
AleI CACnn'nnGTG
Cuts at: 0 733 769 783
Size: 733 36 14
Fragments arranged by size:
733 36 14
AlwNI CAG_nnn'CTG
Cuts at: 0 385 783
Size: 385 398
ApaI G_GGCC'C
Cuts at: 0 646 783
Size: 646 137
ApaLI G'TGCA_C
Cuts at: 0 163 783
Size: 163 620
AvaI C'yCGr_G
Cuts at: 0 627 783
Size: 627 156
BanII G_rGCy'C
Cuts at: 0 395 646 783
Size: 395 251 137
Fragments arranged by size:
395 251 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 257 783
Size: 9 248 526
Fragments arranged by size:
526 248 9
BglI GCCn_nnn'nGGC
Cuts at: 0 714 783
Size: 714 69
Bme1580I G_kGCm'C
Cuts at: 0 167 646 705 783
Size: 167 479 59 78
Fragments arranged by size:
479 167 78 59
BmgBI CAC'GTC
Cuts at: 0 38 546 680 783
Size: 38 508 134 103
Fragments arranged by size:
508 134 103 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 451 783
Size: 165 286 332
Fragments arranged by size:
332 286 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 783
Size: 333 450
BsaWI w'CCGG_w
Cuts at: 0 746 783
Size: 746 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 783
Size: 502 281
BseYI C'CCAG_C
Cuts at: 0 83 222 467 783
Size: 83 139 245 316
Fragments arranged by size:
316 245 139 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 783
Size: 35 748
BsiHKAI G_wGCw'C
Cuts at: 0 167 395 783
Size: 167 228 388
Fragments arranged by size:
388 228 167
BsmI GAATG_Cn'
Cuts at: 0 99 783
Size: 99 684
Bsp1286I G_dGCh'C
Cuts at: 0 167 395 646 705 783
Size: 167 228 251 59 78
Fragments arranged by size:
251 228 167 78 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 105 428 460 631 783
Size: 105 323 32 171 152
Fragments arranged by size:
323 171 152 105 32
BsrBI CCG'CTC
Cuts at: 0 297 783
Size: 297 486
BsrGI T'GTAC_A
Cuts at: 0 725 783
Size: 725 58
BssSI C'ACGA_G
Cuts at: 0 131 783
Size: 131 652
BstEII G'GTnAC_C
Cuts at: 0 573 783
Size: 573 210
Bsu36I CC'TnA_GG
Cuts at: 0 331 462 783
Size: 331 131 321
Fragments arranged by size:
331 321 131
BtsI GCAGTG_nn'
Cuts at: 0 192 696 783
Size: 192 504 87
Fragments arranged by size:
504 192 87
ClaI AT'CG_AT
Cuts at: 0 324 783
Size: 324 459
EaeI y'GGCC_r
Cuts at: 0 773 783
Size: 773 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 361 783
Size: 361 422
EcoICRI GAG'CTC
Cuts at: 0 393 783
Size: 393 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 333 337 496 783
Size: 269 64 4 159 287
Fragments arranged by size:
287 269 159 64 4
EcoO109I rG'GnC_Cy
Cuts at: 0 6 177 642 783
Size: 6 171 465 141
Fragments arranged by size:
465 171 141 6
FspI TGC'GCA
Cuts at: 0 554 783
Size: 554 229
MscI TGG'CCA
Cuts at: 0 775 783
Size: 775 8
MslI CAynn'nnrTG
Cuts at: 0 733 769 783
Size: 733 36 14
Fragments arranged by size:
733 36 14
MspA1I CmG'CkG
Cuts at: 0 187 783
Size: 187 596
PpuMI rG'GwC_Cy
Cuts at: 0 6 177 783
Size: 6 171 606
Fragments arranged by size:
606 171 6
PspOMI G'GGCC_C
Cuts at: 0 642 783
Size: 642 141
PvuII CAG'CTG
Cuts at: 0 187 783
Size: 187 596
SacI G_AGCT'C
Cuts at: 0 395 783
Size: 395 388
SanDI GG'GwC_CC
Cuts at: 0 6 783
Size: 6 777
StuI AGG'CCT
Cuts at: 0 717 783
Size: 717 66
StyI C'CwwG_G
Cuts at: 0 731 783
Size: 731 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 442 783
Size: 442 341
TatI w'GTAC_w
Cuts at: 0 725 783
Size: 725 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 44 76 783
Size: 44 32 707
Fragments arranged by size:
707 44 32
XmnI GAAnn'nnTTC
Cuts at: 0 325 783
Size: 325 458
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BanII BbsI BglI Bme1580I BmgBI BmrI BpmI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrGI BssSI BstEII Bsu36I BtsI ClaI EaeI EciI
EcoICRI Eco57MI EcoO109I FspI MscI MslI MspA1I PpuMI
PspOMI PvuII SacI SanDI StuI StyI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrFI BssHII BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI CspCI DraI DraIII DrdI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI