Restriction map for
pF1KB6330
(Linear) (Six-base) MAPSORT of: KIBB6330.seq Check: 9414 from: 1 to: 864
KIBB6330.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 267 864
Size: 267 597
AatII G_ACGT'C
Cuts at: 0 755 864
Size: 755 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 864
Size: 19 845
AlwNI CAG_nnn'CTG
Cuts at: 0 84 282 579 864
Size: 84 198 297 285
Fragments arranged by size:
297 285 198 84
AvaI C'yCGr_G
Cuts at: 0 97 864
Size: 97 767
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 800 833 864
Size: 800 33 31
Fragments arranged by size:
800 33 31
BamHI G'GATC_C
Cuts at: 0 212 864
Size: 212 652
BanII G_rGCy'C
Cuts at: 0 98 238 864
Size: 98 140 626
Fragments arranged by size:
626 140 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 864
Size: 837 27
BclI T'GATC_A
Cuts at: 0 245 864
Size: 245 619
BglI GCCn_nnn'nGGC
Cuts at: 0 590 864
Size: 590 274
BglII A'GATC_T
Cuts at: 0 473 864
Size: 473 391
BlpI GC'TnA_GC
Cuts at: 0 272 572 864
Size: 272 300 292
Fragments arranged by size:
300 292 272
Bme1580I G_kGCm'C
Cuts at: 0 137 864
Size: 137 727
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 864
Size: 619 245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 656 864
Size: 656 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 864
Size: 778 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 864
Size: 40 824
BsaHI Gr'CG_yC
Cuts at: 0 752 864
Size: 752 112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 328 463 466 864
Size: 55 273 135 3 398
Fragments arranged by size:
398 273 135 55 3
BseYI C'CCAG_C
Cuts at: 0 167 546 864
Size: 167 379 318
Fragments arranged by size:
379 318 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 864
Size: 352 512
BsiHKAI G_wGCw'C
Cuts at: 0 98 299 713 864
Size: 98 201 414 151
Fragments arranged by size:
414 201 151 98
Bsp1286I G_dGCh'C
Cuts at: 0 98 137 238 299 713 864
Size: 98 39 101 61 414 151
Fragments arranged by size:
414 151 101 98 61 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 267 324 864
Size: 83 184 57 540
Fragments arranged by size:
540 184 83 57
BsrBI CCG'CTC
Cuts at: 0 698 864
Size: 698 166
BsrFI r'CCGG_y
Cuts at: 0 320 864
Size: 320 544
BstAPI GCAn_nnn'nTGC
Cuts at: 0 282 864
Size: 282 582
BstEII G'GTnAC_C
Cuts at: 0 360 864
Size: 360 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 864
Size: 397 467
BstYI r'GATC_y
Cuts at: 0 140 212 473 659 761 864
Size: 140 72 261 186 102 103
Fragments arranged by size:
261 186 140 103 102 72
Bsu36I CC'TnA_GG
Cuts at: 0 436 864
Size: 436 428
BtgI C'CryG_G
Cuts at: 0 356 783 864
Size: 356 427 81
Fragments arranged by size:
427 356 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 521 864
Size: 521 343
BtsI GCAGTG_nn'
Cuts at: 0 216 864
Size: 216 648
DraIII CAC_nnn'GTG
Cuts at: 0 613 864
Size: 613 251
EaeI y'GGCC_r
Cuts at: 0 399 591 864
Size: 399 192 273
Fragments arranged by size:
399 273 192
EcoICRI GAG'CTC
Cuts at: 0 96 864
Size: 96 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 778 864
Size: 19 759 86
Fragments arranged by size:
759 86 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 864
Size: 37 827
EcoO109I rG'GnC_Cy
Cuts at: 0 72 120 174 804 834 864
Size: 72 48 54 630 30 30
Fragments arranged by size:
630 72 54 48 30 30
EcoRV GAT'ATC
Cuts at: 0 726 864
Size: 726 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 864
Size: 664 32 168
Fragments arranged by size:
664 168 32
MslI CAynn'nnrTG
Cuts at: 0 373 864
Size: 373 491
MspA1I CmG'CkG
Cuts at: 0 84 785 864
Size: 84 701 79
Fragments arranged by size:
701 84 79
NaeI GCC'GGC
Cuts at: 0 322 864
Size: 322 542
NcoI C'CATG_G
Cuts at: 0 356 864
Size: 356 508
NgoMIV G'CCGG_C
Cuts at: 0 320 864
Size: 320 544
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 378 613 864
Size: 345 33 235 251
Fragments arranged by size:
345 251 235 33
PfoI T'CCnGG_A
Cuts at: 0 755 864
Size: 755 109
PpuMI rG'GwC_Cy
Cuts at: 0 72 120 174 864
Size: 72 48 54 690
Fragments arranged by size:
690 72 54 48
PstI C_TGCA'G
Cuts at: 0 80 864
Size: 80 784
PvuII CAG'CTG
Cuts at: 0 84 864
Size: 84 780
SacI G_AGCT'C
Cuts at: 0 98 864
Size: 98 766
SacII CC_GC'GG
Cuts at: 0 786 864
Size: 786 78
SfcI C'TryA_G
Cuts at: 0 76 864
Size: 76 788
SmlI C'TyrA_G
Cuts at: 0 55 864
Size: 55 809
StyI C'CwwG_G
Cuts at: 0 344 356 822 864
Size: 344 12 466 42
Fragments arranged by size:
466 344 42 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 601 864
Size: 181 420 263
Fragments arranged by size:
420 263 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 408 440 486 518 864
Size: 408 32 46 32 346
Fragments arranged by size:
408 346 46 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 864
Size: 118 746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 375 864
Size: 144 231 489
Fragments arranged by size:
489 231 144
ZraI GAC'GTC
Cuts at: 0 753 864
Size: 753 111
Enzymes that do cut:
AarI AatII AcuI AlwNI AvaI BaeI BamHI BanII
BbsI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV Hin4I MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PstI PvuII SacI SacII SfcI
SmlI StyI TaqII TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BanI BbeI BbvCI BcgI BciVI BfrBI
BmgBI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DrdI
EagI EarI EciI EcoRI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI XbaI XhoI XmaI XmnI