(Linear) (Six-base) MAPSORT of: KIBB6326.seq  Check: 1893  from: 1  to: 858



KIBB6326.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     858 
   Size:        251     607
 
AleI CACnn'nnGTG

Cuts at:      0     433     858 
   Size:        433     425
 
ApoI r'AATT_y

Cuts at:      0     405     858 
   Size:        405     453
 
BanII G_rGCy'C

Cuts at:      0     488     858 
   Size:        488     370
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     558     858 
   Size:        558     300
 
BglI GCCn_nnn'nGGC

Cuts at:      0      77     858 
   Size:         77     781
 
BglII A'GATC_T

Cuts at:      0      99     858 
   Size:         99     759
 
Bme1580I G_kGCm'C

Cuts at:      0     831     858 
   Size:        831      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     699     858 
   Size:        699     159
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     858 
   Size:        266     592
 
Bsp1286I G_dGCh'C

Cuts at:      0     488     831     858 
   Size:        488     343      27

  Fragments arranged by size: 

                 488     343      27
 
BsrDI GCAATG_nn'

Cuts at:      0     552     858 
   Size:        552     306
 
BssSI C'ACGA_G

Cuts at:      0      37     858 
   Size:         37     821
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     189     858 
   Size:        189     669
 
BstYI r'GATC_y

Cuts at:      0      99     847     858 
   Size:         99     748      11

  Fragments arranged by size: 

                 748      99      11
 
Bsu36I CC'TnA_GG

Cuts at:      0     380     770     858 
   Size:        380     390      88

  Fragments arranged by size: 

                 390     380      88
 
BtgI C'CryG_G

Cuts at:      0     488     858 
   Size:        488     370
 
BtsI GCAGTG_nn'

Cuts at:      0      95     858 
   Size:         95     763
 
DraIII CAC_nnn'GTG

Cuts at:      0     244     858 
   Size:        244     614
 
EarI CTCTTCn'nnn_

Cuts at:      0     422     829     858 
   Size:        422     407      29

  Fragments arranged by size: 

                 422     407      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     509     858 
   Size:        509     349
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     858 
   Size:        251     607
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     605     637     858 
   Size:        605      32     221

  Fragments arranged by size: 

                 605     221      32
 
HincII GTy'rAC

Cuts at:      0     753     858 
   Size:        753     105
 
HindIII A'AGCT_T

Cuts at:      0     233     797     858 
   Size:        233     564      61

  Fragments arranged by size: 

                 564     233      61
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     742     858 
   Size:        742     116
 
MslI CAynn'nnrTG

Cuts at:      0     433     858 
   Size:        433     425
 
NcoI C'CATG_G

Cuts at:      0     488     858 
   Size:        488     370
 
PstI C_TGCA'G

Cuts at:      0     483     858 
   Size:        483     375
 
SapI GCTCTTCn'nnn_

Cuts at:      0     422     858 
   Size:        422     436
 
ScaI AGT'ACT

Cuts at:      0     466     858 
   Size:        466     392
 
SfcI C'TryA_G

Cuts at:      0     431     479     858 
   Size:        431      48     379

  Fragments arranged by size: 

                 431     379      48
 
StyI C'CwwG_G

Cuts at:      0     488     858 
   Size:        488     370
 
TatI w'GTAC_w

Cuts at:      0     464     858 
   Size:        464     394
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     402     434     858 
   Size:        402      32     424

  Fragments arranged by size: 

                 424     402      32
 
XbaI T'CTAG_A

Cuts at:      0     691     858 
   Size:        691     167
 
XmnI GAAnn'nnTTC

Cuts at:      0     281     858 
   Size:        281     577
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BanII     BbsI     BglI    BglII Bme1580I
     BsaI     BsgI Bsp1286I    BsrDI    BssSI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EarI     EciI  Eco57MI    Hin4I   HincII
  HindIII     MmeI     MslI     NcoI     PstI     SapI     ScaI     SfcI
     StyI     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XcmI     XhoI     XmaI     ZraI