(Linear) (Six-base) MAPSORT of: KIBB6324.seq  Check: 3742  from: 1  to: 846



KIBB6324.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AclI AA'CG_TT

Cuts at:      0      68     846 
   Size:         68     778
 
AflIII A'CryG_T

Cuts at:      0     121     846 
   Size:        121     725
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     200     846 
   Size:        200     646
 
AseI AT'TA_AT

Cuts at:      0     251     458     846 
   Size:        251     207     388

  Fragments arranged by size: 

                 388     251     207
 
BanII G_rGCy'C

Cuts at:      0     488     846 
   Size:        488     358
 
BclI T'GATC_A

Cuts at:      0      36     846 
   Size:         36     810
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     415     846 
   Size:        415     431
 
BsaAI yAC'GTr

Cuts at:      0     122     846 
   Size:        122     724
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     368     398     528     558     846 
   Size:        368      30     130      30     288

  Fragments arranged by size: 

                 368     288     130      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     392     433     846 
   Size:        392      41     413

  Fragments arranged by size: 

                 413     392      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     846 
   Size:        302     544
 
Bsp1286I G_dGCh'C

Cuts at:      0     488     846 
   Size:        488     358
 
BstBI TT'CG_AA

Cuts at:      0     246     324     846 
   Size:        246      78     522

  Fragments arranged by size: 

                 522     246      78
 
BstEII G'GTnAC_C

Cuts at:      0     346     846 
   Size:        346     500
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     343     846 
   Size:        343     503
 
BstYI r'GATC_y

Cuts at:      0      20     846 
   Size:         20     826
 
Bsu36I CC'TnA_GG

Cuts at:      0     416     846 
   Size:        416     430
 
DraI TTT'AAA

Cuts at:      0     219     846 
   Size:        219     627
 
EaeI y'GGCC_r

Cuts at:      0      53     846 
   Size:         53     793
 
EarI CTCTTCn'nnn_

Cuts at:      0     224     846 
   Size:        224     622
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     302     846 
   Size:        302     544
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     693     846 
   Size:        693     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     262     294     846 
   Size:        262      32     552

  Fragments arranged by size: 

                 552     262      32
 
HincII GTy'rAC

Cuts at:      0     660     846 
   Size:        660     186
 
MscI TGG'CCA

Cuts at:      0      55     846 
   Size:         55     791
 
NspI r_CATG'y

Cuts at:      0     118     846 
   Size:        118     728
 
PfoI T'CCnGG_A

Cuts at:      0     365     846 
   Size:        365     481
 
PmlI CAC'GTG

Cuts at:      0     122     846 
   Size:        122     724
 
PshAI GACnn'nnGTC

Cuts at:      0     655     846 
   Size:        655     191
 
PstI C_TGCA'G

Cuts at:      0     445     476     846 
   Size:        445      31     370

  Fragments arranged by size: 

                 445     370      31
 
SfcI C'TryA_G

Cuts at:      0     441     472     846 
   Size:        441      31     374

  Fragments arranged by size: 

                 441     374      31
 
SspI AAT'ATT

Cuts at:      0     206     805     846 
   Size:        206     599      41

  Fragments arranged by size: 

                 599     206      41
 
StyI C'CwwG_G

Cuts at:      0     336     846 
   Size:        336     510
 
TatI w'GTAC_w

Cuts at:      0      41     846 
   Size:         41     805
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     368     400     846 
   Size:        368      32     446

  Fragments arranged by size: 

                 446     368      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     635     827     846 
   Size:        635     192      19

  Fragments arranged by size: 

                 635     192      19
 

 Enzymes that do cut:

     AclI   AflIII     AlfI     AseI    BanII     BclI     BsaI    BsaAI
    BsaXI    BseRI     BsgI Bsp1286I    BstBI   BstEII    BstXI    BstYI
   Bsu36I     DraI     EaeI     EarI    EcoNI     FalI    Hin4I   HincII
     MscI     NspI     PfoI     PmlI    PshAI     PstI     SfcI     SspI
     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI