(Linear) (Six-base) MAPSORT of: KIBB6317.seq  Check: 4487  from: 1  to: 780



KIBB6317.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     458     508     780 
   Size:        161     297      50     272

  Fragments arranged by size: 

                 297     272     161      50
 
AlwNI CAG_nnn'CTG

Cuts at:      0     220     780 
   Size:        220     560
 
ApoI r'AATT_y

Cuts at:      0     356     396     780 
   Size:        356      40     384

  Fragments arranged by size: 

                 384     356      40
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     139     780 
   Size:        139     641
 
BfrBI ATG'CAT

Cuts at:      0     260     780 
   Size:        260     520
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     780 
   Size:        305     475
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     172     780 
   Size:         34     138     608

  Fragments arranged by size: 

                 608     138      34
 
BseYI C'CCAG_C

Cuts at:      0     453     780 
   Size:        453     327
 
BsmI GAATG_Cn'

Cuts at:      0     262     780 
   Size:        262     518
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     126     780 
   Size:        126     654
 
BsrBI CCG'CTC

Cuts at:      0      42     780 
   Size:         42     738
 
BstEII G'GTnAC_C

Cuts at:      0     174     780 
   Size:        174     606
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     283     780 
   Size:        283     497
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     373     408     780 
   Size:        373      35     372

  Fragments arranged by size: 

                 373     372      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     780 
   Size:         99     681
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     305     458     508     780 
   Size:        161     144     153      50     272

  Fragments arranged by size: 

                 272     161     153     144      50
 
EcoRI G'AATT_C

Cuts at:      0     396     780 
   Size:        396     384
 
EcoRV GAT'ATC

Cuts at:      0     474     780 
   Size:        474     306
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     166     780 
   Size:        166     614
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     168     200     399     431     433     465     520     552 
   Size:        168      32     199      32       2      32      55      32

Cuts at:    552     592     624     780 
   Size:         40      32     156

  Fragments arranged by size: 

                 199     168     156      55      40      32      32      32
                  32      32       2
 
MspA1I CmG'CkG

Cuts at:      0      13     750     780 
   Size:         13     737      30

  Fragments arranged by size: 

                 737      30      13
 
NruI TCG'CGA

Cuts at:      0      38     780 
   Size:         38     742
 
NsiI A_TGCA'T

Cuts at:      0     262     780 
   Size:        262     518
 
PvuII CAG'CTG

Cuts at:      0     750     780 
   Size:        750      30
 
SmlI C'TyrA_G

Cuts at:      0      49     151     780 
   Size:         49     102     629

  Fragments arranged by size: 

                 629     102      49
 
TatI w'GTAC_w

Cuts at:      0     662     780 
   Size:        662     118
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BciVI    BfrBI     BpmI    BpuEI    BseYI
     BsmI    BspMI    BsrBI   BstEII    BstXI    CspCI   DraIII  Eco57MI
    EcoRI    EcoRV     FalI    Hin4I   MspA1I     NruI     NsiI    PvuII
     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI