Restriction map for
pF1KB6312
(Linear) (Six-base) MAPSORT of: KIBB6312.seq Check: 9195 from: 1 to: 840
KIBB6312.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 840
Size: 767 73
AlwNI CAG_nnn'CTG
Cuts at: 0 246 840
Size: 246 594
AvrII C'CTAG_G
Cuts at: 0 7 840
Size: 7 833
BamHI G'GATC_C
Cuts at: 0 762 840
Size: 762 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 579 840
Size: 579 261
BglII A'GATC_T
Cuts at: 0 677 840
Size: 677 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 596 840
Size: 539 57 244
Fragments arranged by size:
539 244 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 564 840
Size: 124 440 276
Fragments arranged by size:
440 276 124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 840
Size: 127 713
BseYI C'CCAG_C
Cuts at: 0 513 755 840
Size: 513 242 85
Fragments arranged by size:
513 242 85
BsiEI CG_ry'CG
Cuts at: 0 87 840
Size: 87 753
BsmBI CGTCTCn'nnnn_
Cuts at: 0 808 840
Size: 808 32
BsrBI CCG'CTC
Cuts at: 0 627 840
Size: 627 213
BstYI r'GATC_y
Cuts at: 0 170 677 762 840
Size: 170 507 85 78
Fragments arranged by size:
507 170 85 78
BtgI C'CryG_G
Cuts at: 0 518 539 840
Size: 518 21 301
Fragments arranged by size:
518 301 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 583 840
Size: 583 257
EaeI y'GGCC_r
Cuts at: 0 29 725 840
Size: 29 696 115
Fragments arranged by size:
696 115 29
EarI CTCTTCn'nnn_
Cuts at: 0 245 368 442 840
Size: 245 123 74 398
Fragments arranged by size:
398 245 123 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 719 840
Size: 69 650 121
Fragments arranged by size:
650 121 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 596 840
Size: 539 57 244
Fragments arranged by size:
539 244 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 48 139 142 250 840
Size: 48 91 3 108 590
Fragments arranged by size:
590 108 91 48 3
EcoO109I rG'GnC_Cy
Cuts at: 0 243 840
Size: 243 597
EcoRV GAT'ATC
Cuts at: 0 276 840
Size: 276 564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 840
Size: 821 19
MscI TGG'CCA
Cuts at: 0 31 727 840
Size: 31 696 113
Fragments arranged by size:
696 113 31
MspA1I CmG'CkG
Cuts at: 0 265 759 840
Size: 265 494 81
Fragments arranged by size:
494 265 81
NcoI C'CATG_G
Cuts at: 0 539 840
Size: 539 301
PstI C_TGCA'G
Cuts at: 0 820 840
Size: 820 20
PvuII CAG'CTG
Cuts at: 0 265 759 840
Size: 265 494 81
Fragments arranged by size:
494 265 81
SfcI C'TryA_G
Cuts at: 0 816 840
Size: 816 24
SmlI C'TyrA_G
Cuts at: 0 139 579 840
Size: 139 440 261
Fragments arranged by size:
440 261 139
StuI AGG'CCT
Cuts at: 0 560 772 840
Size: 560 212 68
Fragments arranged by size:
560 212 68
StyI C'CwwG_G
Cuts at: 0 7 185 225 539 773 840
Size: 7 178 40 314 234 67
Fragments arranged by size:
314 234 178 67 40 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 582 840
Size: 582 258
XmnI GAAnn'nnTTC
Cuts at: 0 79 308 840
Size: 79 229 532
Fragments arranged by size:
532 229 79
Enzymes that do cut:
AlfI AlwNI AvrII BamHI BbsI BglII BpmI BpuEI
BseRI BseYI BsiEI BsmBI BsrBI BstYI BtgI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV MmeI
MscI MspA1I NcoI PstI PvuII SfcI SmlI StuI
StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BaeI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EcoICRI EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI