(Linear) (Six-base) MAPSORT of: KIBB6304.seq  Check: 8440  from: 1  to: 834



KIBB6304.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:      0     152     468     505     818     834 
   Size:        152     316      37     313      16

  Fragments arranged by size: 

                 316     313     152      37      16
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      50     834 
   Size:         50     784
 
ApoI r'AATT_y

Cuts at:      0     240     435     571     834 
   Size:        240     195     136     263

  Fragments arranged by size: 

                 263     240     195     136
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     318     834 
   Size:        318     516
 
BmtI G_CTAG'C

Cuts at:      0     337     834 
   Size:        337     497
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737     834 
   Size:        737      97
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     613     834 
   Size:         28     585     221

  Fragments arranged by size: 

                 585     221      28
 
BsmI GAATG_Cn'

Cuts at:      0     291     834 
   Size:        291     543
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     749     834 
   Size:        749      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      18     834 
   Size:         18     816
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     621     834 
   Size:        621     213
 
BtgI C'CryG_G

Cuts at:      0      91     834 
   Size:         91     743
 
BtsI GCAGTG_nn'

Cuts at:      0     359     834 
   Size:        359     475
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     148     834 
   Size:        148     686
 
EarI CTCTTCn'nnn_

Cuts at:      0     807     834 
   Size:        807      27
 
EcoRI G'AATT_C

Cuts at:      0     571     834 
   Size:        571     263
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     820     834 
   Size:        820      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128     352     357     384     389     569     601 
   Size:         96      32     224       5      27       5     180      32

Cuts at:    601     834 
   Size:        233

  Fragments arranged by size: 

                 233     224     180      96      32      32      27       5
                   5
 
HincII GTy'rAC

Cuts at:      0     297     834 
   Size:        297     537
 
NheI G'CTAG_C

Cuts at:      0     333     834 
   Size:        333     501
 
PfoI T'CCnGG_A

Cuts at:      0     137     834 
   Size:        137     697
 
PstI C_TGCA'G

Cuts at:      0     354     597     834 
   Size:        354     243     237

  Fragments arranged by size: 

                 354     243     237
 
SexAI A'CCwGG_T

Cuts at:      0     155     834 
   Size:        155     679
 
SfcI C'TryA_G

Cuts at:      0     350     506     593     834 
   Size:        350     156      87     241

  Fragments arranged by size: 

                 350     241     156      87
 
SmlI C'TyrA_G

Cuts at:      0     752     834 
   Size:        752      82
 
SspI AAT'ATT

Cuts at:      0      53     834 
   Size:         53     781
 

 Enzymes that do cut:

     AccI     AlfI     ApoI    BciVI     BmtI    BpuEI    BseRI     BsmI
    BsmBI    BspMI    BstXI     BtgI     BtsI     DrdI     EarI    EcoRI
     FalI    Hin4I   HincII     NheI     PfoI     PstI    SexAI     SfcI
     SmlI     SspI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI