(Linear) (Six-base) MAPSORT of: KIBB6302.seq  Check: 2694  from: 1  to: 831



KIBB6302.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     831 
   Size:        207     624
 
AleI CACnn'nnGTG

Cuts at:      0     557     831 
   Size:        557     274
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     428     831 
   Size:        428     403
 
AlwNI CAG_nnn'CTG

Cuts at:      0      20     636     831 
   Size:         20     616     195

  Fragments arranged by size: 

                 616     195      20
 
BglII A'GATC_T

Cuts at:      0     545     831 
   Size:        545     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     831 
   Size:         42     789
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      29      59     831 
   Size:         29      30     772

  Fragments arranged by size: 

                 772      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     490     808     831 
   Size:        490     318      23

  Fragments arranged by size: 

                 490     318      23
 
BseYI C'CCAG_C

Cuts at:      0     786     831 
   Size:        786      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     831 
   Size:        434     397
 
BsiHKAI G_wGCw'C

Cuts at:      0     187     831 
   Size:        187     644
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     831 
   Size:        187     644
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     256     657     831 
   Size:        256     401     174

  Fragments arranged by size: 

                 401     256     174
 
BstYI r'GATC_y

Cuts at:      0     545     690     831 
   Size:        545     145     141

  Fragments arranged by size: 

                 545     145     141
 
BtsI GCAGTG_nn'

Cuts at:      0      65     422     831 
   Size:         65     357     409

  Fragments arranged by size: 

                 409     357      65
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     375     410     831 
   Size:        375      35     421

  Fragments arranged by size: 

                 421     375      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     142     831 
   Size:        142     689
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     207     831 
   Size:         42     165     624

  Fragments arranged by size: 

                 624     165      42
 
HindIII A'AGCT_T

Cuts at:      0     174     831 
   Size:        174     657
 
MslI CAynn'nnrTG

Cuts at:      0     557     831 
   Size:        557     274
 
MspA1I CmG'CkG

Cuts at:      0      17     786     831 
   Size:         17     769      45

  Fragments arranged by size: 

                 769      45      17
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     636     831 
   Size:        636     195
 
PstI C_TGCA'G

Cuts at:      0     759     831 
   Size:        759      72
 
PvuII CAG'CTG

Cuts at:      0      17     786     831 
   Size:         17     769      45

  Fragments arranged by size: 

                 769      45      17
 
SexAI A'CCwGG_T

Cuts at:      0     701     831 
   Size:        701     130
 
SfcI C'TryA_G

Cuts at:      0      12     755     831 
   Size:         12     743      76

  Fragments arranged by size: 

                 743      76      12
 
StuI AGG'CCT

Cuts at:      0     266     831 
   Size:        266     565
 
Tth111I GACn'n_nGTC

Cuts at:      0     724     831 
   Size:        724     107
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI    BglII     BpmI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BstXI    BstYI     BtsI    CspCI
     EarI  Eco57MI  HindIII     MslI   MspA1I    PflMI     PstI    PvuII
    SexAI     SfcI     StuI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI