Restriction map for
pF1KB6290
(Linear) (Six-base) MAPSORT of: KIBB6290.seq Check: 1656 from: 1 to: 2355
KIBB6290.orf
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1729 1822 1934 2301 2355
Size: 1729 93 112 367 54
Fragments arranged by size:
1729 367 112 93 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1601 2204 2355
Size: 1303 298 603 151
Fragments arranged by size:
1303 603 298 151
AfeI AGC'GCT
Cuts at: 0 2080 2355
Size: 2080 275
AflIII A'CryG_T
Cuts at: 0 2002 2355
Size: 2002 353
AhdI GACnn_n'nnGTC
Cuts at: 0 2100 2355
Size: 2100 255
AleI CACnn'nnGTG
Cuts at: 0 2042 2355
Size: 2042 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 216 584 1830 2355
Size: 216 368 1246 525
Fragments arranged by size:
1246 525 368 216
AlwNI CAG_nnn'CTG
Cuts at: 0 427 1549 2355
Size: 427 1122 806
Fragments arranged by size:
1122 806 427
ApaI G_GGCC'C
Cuts at: 0 250 582 1373 2355
Size: 250 332 791 982
Fragments arranged by size:
982 791 332 250
ApaLI G'TGCA_C
Cuts at: 0 1630 2355
Size: 1630 725
ApoI r'AATT_y
Cuts at: 0 1193 2053 2355
Size: 1193 860 302
Fragments arranged by size:
1193 860 302
AseI AT'TA_AT
Cuts at: 0 2333 2355
Size: 2333 22
AvaI C'yCGr_G
Cuts at: 0 249 1848 2355
Size: 249 1599 507
Fragments arranged by size:
1599 507 249
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 749 782 2355
Size: 749 33 1573
Fragments arranged by size:
1573 749 33
BamHI G'GATC_C
Cuts at: 0 1854 2355
Size: 1854 501
BanI G'GyrC_C
Cuts at: 0 97 307 2355
Size: 97 210 2048
Fragments arranged by size:
2048 210 97
BanII G_rGCy'C
Cuts at: 0 250 582 1373 1689 1849 2355
Size: 250 332 791 316 160 506
Fragments arranged by size:
791 506 332 316 250 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 2355
Size: 398 1957
BbvCI CC'TCA_GC
Cuts at: 0 1208 1518 2355
Size: 1208 310 837
Fragments arranged by size:
1208 837 310
BclI T'GATC_A
Cuts at: 0 1677 2355
Size: 1677 678
BglI GCCn_nnn'nGGC
Cuts at: 0 577 1237 2254 2355
Size: 577 660 1017 101
Fragments arranged by size:
1017 660 577 101
BglII A'GATC_T
Cuts at: 0 2319 2355
Size: 2319 36
BlpI GC'TnA_GC
Cuts at: 0 1688 2355
Size: 1688 667
Bme1580I G_kGCm'C
Cuts at: 0 250 582 1373 1634 1935 2167 2355
Size: 250 332 791 261 301 232 188
Fragments arranged by size:
791 332 301 261 250 232 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 584 2355
Size: 584 1771
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 900 2355
Size: 900 1455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 515 679 1148 1219 1288 1297 1570
Size: 167 348 164 469 71 69 9 273
Cuts at: 1570 2355
Size: 785
Fragments arranged by size:
785 469 348 273 167 164 71 69
9
Bpu10I CC'TnA_GC
Cuts at: 0 1208 1449 1518 2075 2355
Size: 1208 241 69 557 280
Fragments arranged by size:
1208 557 280 241 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1265 2355
Size: 1037 228 1090
Fragments arranged by size:
1090 1037 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 1702 2355
Size: 1702 653
BsaWI w'CCGG_w
Cuts at: 0 1352 2355
Size: 1352 1003
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 816 846 1217 1247 2199 2229 2355
Size: 816 30 371 30 952 30 126
Fragments arranged by size:
952 816 371 126 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 167 327 1560 2103 2355
Size: 164 3 160 1233 543 252
Fragments arranged by size:
1233 543 252 164 160 3
BseYI C'CCAG_C
Cuts at: 0 167 835 2355
Size: 167 668 1520
Fragments arranged by size:
1520 668 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 2355
Size: 607 1748
BsiEI CG_ry'CG
Cuts at: 0 1788 2355
Size: 1788 567
BsiHKAI G_wGCw'C
Cuts at: 0 1634 1689 2355
Size: 1634 55 666
Fragments arranged by size:
1634 666 55
BsmI GAATG_Cn'
Cuts at: 0 1560 2355
Size: 1560 795
Bsp1286I G_dGCh'C
Cuts at: 0 250 582 1373 1634 1689 1849 1935 2167
Size: 250 332 791 261 55 160 86 232
Cuts at: 2167 2355
Size: 188
Fragments arranged by size:
791 332 261 250 232 188 160 86
55
BspEI T'CCGG_A
Cuts at: 0 1352 2355
Size: 1352 1003
BspHI T'CATG_A
Cuts at: 0 2084 2355
Size: 2084 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1729 1822 1934 2179 2301 2355
Size: 1729 93 112 245 122 54
Fragments arranged by size:
1729 245 122 112 93 54
BsrBI CCG'CTC
Cuts at: 0 454 2355
Size: 454 1901
BsrDI GCAATG_nn'
Cuts at: 0 87 399 671 1135 1511 2273 2352 2355
Size: 87 312 272 464 376 762 79 3
Fragments arranged by size:
762 464 376 312 272 87 79 3
BsrFI r'CCGG_y
Cuts at: 0 1783 2355
Size: 1783 572
BstAPI GCAn_nnn'nTGC
Cuts at: 0 558 2355
Size: 558 1797
BstXI CCAn_nnnn'nTGG
Cuts at: 0 880 1005 1126 1708 1897 2241 2355
Size: 880 125 121 582 189 344 114
Fragments arranged by size:
880 582 344 189 125 121 114
BstYI r'GATC_y
Cuts at: 0 1854 2319 2355
Size: 1854 465 36
Fragments arranged by size:
1854 465 36
Bsu36I CC'TnA_GG
Cuts at: 0 900 2355
Size: 900 1455
BtgI C'CryG_G
Cuts at: 0 20 1701 2249 2355
Size: 20 1681 548 106
Fragments arranged by size:
1681 548 106 20
BtsI GCAGTG_nn'
Cuts at: 0 405 2207 2208 2355
Size: 405 1802 1 147
Fragments arranged by size:
1802 405 147 1
DraIII CAC_nnn'GTG
Cuts at: 0 307 2355
Size: 307 2048
EarI CTCTTCn'nnn_
Cuts at: 0 336 2355
Size: 336 2019
EcoICRI GAG'CTC
Cuts at: 0 1687 2355
Size: 1687 668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 515 679 1148 1219 1288 1297 1303
Size: 167 348 164 469 71 69 9 6
Cuts at: 1303 1570 1601 2204 2355
Size: 267 31 603 151
Fragments arranged by size:
603 469 348 267 167 164 151 71
69 31 9 6
EcoO109I rG'GnC_Cy
Cuts at: 0 246 578 869 1230 1239 1369 1793 2355
Size: 246 332 291 361 9 130 424 562
Fragments arranged by size:
562 424 361 332 291 246 130 9
HaeII r_GCGC'y
Cuts at: 0 2082 2355
Size: 2082 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 653 685 1035 1067 1217 1249 2086 2118
Size: 653 32 350 32 150 32 837 32
Cuts at: 2118 2311 2343 2355
Size: 193 32 12
Fragments arranged by size:
837 653 350 193 150 32 32 32
32 32 12
HincII GTy'rAC
Cuts at: 0 807 2355
Size: 807 1548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 2355
Size: 1768 587
MslI CAynn'nnrTG
Cuts at: 0 1968 2042 2239 2311 2355
Size: 1968 74 197 72 44
Fragments arranged by size:
1968 197 74 72 44
MspA1I CmG'CkG
Cuts at: 0 37 558 1404 1522 2355
Size: 37 521 846 118 833
Fragments arranged by size:
846 833 521 118 37
NcoI C'CATG_G
Cuts at: 0 20 1701 2249 2355
Size: 20 1681 548 106
Fragments arranged by size:
1681 548 106 20
NspI r_CATG'y
Cuts at: 0 1811 2355
Size: 1811 544
PasI CC'CwG_GG
Cuts at: 0 243 2355
Size: 243 2112
PflMI CCAn_nnn'nTGG
Cuts at: 0 317 720 1336 2355
Size: 317 403 616 1019
Fragments arranged by size:
1019 616 403 317
PpuMI rG'GwC_Cy
Cuts at: 0 869 1793 2355
Size: 869 924 562
Fragments arranged by size:
924 869 562
PspOMI G'GGCC_C
Cuts at: 0 246 578 1369 2355
Size: 246 332 791 986
Fragments arranged by size:
986 791 332 246
PstI C_TGCA'G
Cuts at: 0 563 822 1409 2327 2355
Size: 563 259 587 918 28
Fragments arranged by size:
918 587 563 259 28
PvuII CAG'CTG
Cuts at: 0 37 558 1404 1522 2355
Size: 37 521 846 118 833
Fragments arranged by size:
846 833 521 118 37
SacI G_AGCT'C
Cuts at: 0 1689 2355
Size: 1689 666
SanDI GG'GwC_CC
Cuts at: 0 869 2355
Size: 869 1486
SexAI A'CCwGG_T
Cuts at: 0 621 1975 2355
Size: 621 1354 380
Fragments arranged by size:
1354 621 380
SfcI C'TryA_G
Cuts at: 0 559 818 1084 1399 1405 1658 2017 2323
Size: 559 259 266 315 6 253 359 306
Cuts at: 2323 2355
Size: 32
Fragments arranged by size:
559 359 315 306 266 259 253 32
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1237 2355
Size: 1237 1118
SmlI C'TyrA_G
Cuts at: 0 1052 1280 2355
Size: 1052 228 1075
Fragments arranged by size:
1075 1052 228
SphI G_CATG'C
Cuts at: 0 1811 2355
Size: 1811 544
SspI AAT'ATT
Cuts at: 0 510 2355
Size: 510 1845
StuI AGG'CCT
Cuts at: 0 719 1583 1646 2355
Size: 719 864 63 709
Fragments arranged by size:
864 719 709 63
StyI C'CwwG_G
Cuts at: 0 20 714 1472 1701 1890 2249 2355
Size: 20 694 758 229 189 359 106
Fragments arranged by size:
758 694 359 229 189 106 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 1524 1649 2355
Size: 234 1290 125 706
Fragments arranged by size:
1290 706 234 125
TatI w'GTAC_w
Cuts at: 0 732 2355
Size: 732 1623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1613 1645 2199 2231 2355
Size: 1613 32 554 32 124
Fragments arranged by size:
1613 554 124 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 544 768 1002 1075 1708 2355
Size: 544 224 234 73 633 647
Fragments arranged by size:
647 633 544 234 224 73
XmnI GAAnn'nnTTC
Cuts at: 0 1996 2355
Size: 1996 359
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII MmeI MslI MspA1I NcoI NspI
PasI PflMI PpuMI PspOMI PstI PvuII SacI SanDI
SexAI SfcI SfiI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI AscI
AsiSI AvrII BbeI BcgI BciVI BfrBI BmgBI BmtI
BsaAI BsaBI BsaHI BsiWI BsmBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI
EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XbaI XhoI XmaI ZraI