Restriction map for
pF1KB6285
(Linear) (Six-base) MAPSORT of: KIBB6285.seq Check: 5747 from: 1 to: 2586
KIBB6285.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1276 1406 1589 2586
Size: 685 591 130 183 997
Fragments arranged by size:
997 685 591 183 130
AflII C'TTAA_G
Cuts at: 0 967 2245 2586
Size: 967 1278 341
Fragments arranged by size:
1278 967 341
AleI CACnn'nnGTG
Cuts at: 0 115 2586
Size: 115 2471
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2035 2067 2586
Size: 2035 32 519
Fragments arranged by size:
2035 519 32
AlwNI CAG_nnn'CTG
Cuts at: 0 155 183 736 2586
Size: 155 28 553 1850
Fragments arranged by size:
1850 553 155 28
ApaI G_GGCC'C
Cuts at: 0 1249 1537 2586
Size: 1249 288 1049
Fragments arranged by size:
1249 1049 288
ApoI r'AATT_y
Cuts at: 0 904 1763 1988 2179 2586
Size: 904 859 225 191 407
Fragments arranged by size:
904 859 407 225 191
AseI AT'TA_AT
Cuts at: 0 505 746 2586
Size: 505 241 1840
Fragments arranged by size:
1840 505 241
AvaI C'yCGr_G
Cuts at: 0 57 2558 2586
Size: 57 2501 28
Fragments arranged by size:
2501 57 28
AvrII C'CTAG_G
Cuts at: 0 256 2586
Size: 256 2330
BanI G'GyrC_C
Cuts at: 0 28 127 2586
Size: 28 99 2459
Fragments arranged by size:
2459 99 28
BanII G_rGCy'C
Cuts at: 0 167 710 1249 1537 1977 2565 2586
Size: 167 543 539 288 440 588 21
Fragments arranged by size:
588 543 539 440 288 167 21
BbeI G_GCGC'C
Cuts at: 0 32 131 2586
Size: 32 99 2455
Fragments arranged by size:
2455 99 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 929 1432 1684 2586
Size: 929 503 252 902
Fragments arranged by size:
929 902 503 252
BbvCI CC'TCA_GC
Cuts at: 0 392 944 2586
Size: 392 552 1642
Fragments arranged by size:
1642 552 392
BlpI GC'TnA_GC
Cuts at: 0 2108 2586
Size: 2108 478
Bme1580I G_kGCm'C
Cuts at: 0 1249 1537 2586
Size: 1249 288 1049
Fragments arranged by size:
1249 1049 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 1169 1241 1490 1706 2586
Size: 323 846 72 249 216 880
Fragments arranged by size:
880 846 323 249 216 72
Bpu10I CC'TnA_GC
Cuts at: 0 392 944 2586
Size: 392 552 1642
Fragments arranged by size:
1642 552 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 916 1101 2586
Size: 420 496 185 1485
Fragments arranged by size:
1485 496 420 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 1347 2391 2586
Size: 409 938 1044 195
Fragments arranged by size:
1044 938 409 195
BsaHI Gr'CG_yC
Cuts at: 0 29 128 172 2586
Size: 29 99 44 2414
Fragments arranged by size:
2414 99 44 29
BsaWI w'CCGG_w
Cuts at: 0 114 222 2586
Size: 114 108 2364
Fragments arranged by size:
2364 114 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 126 156 615 645 1074 1104 2035 2065
Size: 126 30 459 30 429 30 931 30
Cuts at: 2065 2536 2566 2586
Size: 471 30 20
Fragments arranged by size:
931 471 459 429 126 30 30 30
30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 260 380 2586
Size: 260 120 2206
Fragments arranged by size:
2206 260 120
BseYI C'CCAG_C
Cuts at: 0 176 1471 1526 1555 2586
Size: 176 1295 55 29 1031
Fragments arranged by size:
1295 1031 176 55 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1448 2586
Size: 1448 1138
BsiEI CG_ry'CG
Cuts at: 0 83 2586
Size: 83 2503
BsiHKAI G_wGCw'C
Cuts at: 0 112 1977 2565 2586
Size: 112 1865 588 21
Fragments arranged by size:
1865 588 112 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2345 2586
Size: 2345 241
Bsp1286I G_dGCh'C
Cuts at: 0 112 167 710 1249 1537 1977 2565 2586
Size: 112 55 543 539 288 440 588 21
Fragments arranged by size:
588 543 539 440 288 112 55 21
BsrBI CCG'CTC
Cuts at: 0 34 2586
Size: 34 2552
BsrDI GCAATG_nn'
Cuts at: 0 806 1033 1058 1620 2586
Size: 806 227 25 562 966
Fragments arranged by size:
966 806 562 227 25
BsrFI r'CCGG_y
Cuts at: 0 7 25 93 2586
Size: 7 18 68 2493
Fragments arranged by size:
2493 68 18 7
BsrGI T'GTAC_A
Cuts at: 0 478 2586
Size: 478 2108
BssSI C'ACGA_G
Cuts at: 0 2496 2586
Size: 2496 90
BstEII G'GTnAC_C
Cuts at: 0 207 564 2586
Size: 207 357 2022
Fragments arranged by size:
2022 357 207
BstYI r'GATC_y
Cuts at: 0 2277 2586
Size: 2277 309
Bsu36I CC'TnA_GG
Cuts at: 0 2509 2586
Size: 2509 77
BtgI C'CryG_G
Cuts at: 0 71 167 194 1337 2586
Size: 71 96 27 1143 1249
Fragments arranged by size:
1249 1143 96 71 27
BtsI GCAGTG_nn'
Cuts at: 0 527 1345 2586
Size: 527 818 1241
Fragments arranged by size:
1241 818 527
EaeI y'GGCC_r
Cuts at: 0 80 2586
Size: 80 2506
EagI C'GGCC_G
Cuts at: 0 80 2586
Size: 80 2506
EarI CTCTTCn'nnn_
Cuts at: 0 2389 2586
Size: 2389 197
EcoICRI GAG'CTC
Cuts at: 0 1975 2563 2586
Size: 1975 588 23
Fragments arranged by size:
1975 588 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 685 1169 1241 1276 1406 1490 1589
Size: 323 362 484 72 35 130 84 99
Cuts at: 1589 1706 2586
Size: 117 880
Fragments arranged by size:
880 484 362 323 130 117 99 84
72 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1607 2586
Size: 1607 979
EcoO109I rG'GnC_Cy
Cuts at: 0 200 1534 2586
Size: 200 1334 1052
Fragments arranged by size:
1334 1052 200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2452 2586
Size: 2452 134
HaeII r_GCGC'y
Cuts at: 0 32 56 91 131 233 2586
Size: 32 24 35 40 102 2353
Fragments arranged by size:
2353 102 40 35 32 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 2152 2184 2586
Size: 126 32 1994 32 402
Fragments arranged by size:
1994 402 126 32 32
HincII GTy'rAC
Cuts at: 0 1875 2586
Size: 1875 711
KasI G'GCGC_C
Cuts at: 0 28 127 2586
Size: 28 99 2459
Fragments arranged by size:
2459 99 28
MfeI C'AATT_G
Cuts at: 0 2102 2200 2323 2586
Size: 2102 98 123 263
Fragments arranged by size:
2102 263 123 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 2586
Size: 781 1805
MslI CAynn'nnrTG
Cuts at: 0 115 2586
Size: 115 2471
MspA1I CmG'CkG
Cuts at: 0 251 736 1276 1555 2289 2586
Size: 251 485 540 279 734 297
Fragments arranged by size:
734 540 485 297 279 251
NaeI GCC'GGC
Cuts at: 0 9 27 2586
Size: 9 18 2559
Fragments arranged by size:
2559 18 9
NarI GG'CG_CC
Cuts at: 0 29 128 2586
Size: 29 99 2458
Fragments arranged by size:
2458 99 29
NcoI C'CATG_G
Cuts at: 0 71 2586
Size: 71 2515
NgoMIV G'CCGG_C
Cuts at: 0 7 25 2586
Size: 7 18 2561
Fragments arranged by size:
2561 18 7
NspI r_CATG'y
Cuts at: 0 821 2586
Size: 821 1765
PasI CC'CwG_GG
Cuts at: 0 123 2586
Size: 123 2463
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 2586
Size: 1198 1388
PfoI T'CCnGG_A
Cuts at: 0 339 633 2586
Size: 339 294 1953
Fragments arranged by size:
1953 339 294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 2035 2067 2586
Size: 481 32 1522 32 519
Fragments arranged by size:
1522 519 481 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 200 2586
Size: 200 2386
PshAI GACnn'nnGTC
Cuts at: 0 1339 2586
Size: 1339 1247
PspOMI G'GGCC_C
Cuts at: 0 1245 1533 2586
Size: 1245 288 1053
Fragments arranged by size:
1245 1053 288
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 557 589 2586
Size: 557 32 1997
Fragments arranged by size:
1997 557 32
PvuII CAG'CTG
Cuts at: 0 736 1276 1555 2289 2586
Size: 736 540 279 734 297
Fragments arranged by size:
736 734 540 297 279
SacI G_AGCT'C
Cuts at: 0 1977 2565 2586
Size: 1977 588 21
Fragments arranged by size:
1977 588 21
SanDI GG'GwC_CC
Cuts at: 0 200 2586
Size: 200 2386
SexAI A'CCwGG_T
Cuts at: 0 953 2164 2586
Size: 953 1211 422
Fragments arranged by size:
1211 953 422
SfcI C'TryA_G
Cuts at: 0 1106 2586
Size: 1106 1480
SfoI GGC'GCC
Cuts at: 0 30 129 2586
Size: 30 99 2457
Fragments arranged by size:
2457 99 30
SgrAI Cr'CCGG_yG
Cuts at: 0 25 2586
Size: 25 2561
SmlI C'TyrA_G
Cuts at: 0 435 931 967 1116 2245 2558 2586
Size: 435 496 36 149 1129 313 28
Fragments arranged by size:
1129 496 435 313 149 36 28
SpeI A'CTAG_T
Cuts at: 0 1587 1708 2586
Size: 1587 121 878
Fragments arranged by size:
1587 878 121
StuI AGG'CCT
Cuts at: 0 2081 2586
Size: 2081 505
StyI C'CwwG_G
Cuts at: 0 71 256 2386 2586
Size: 71 185 2130 200
Fragments arranged by size:
2130 200 185 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1176 2586
Size: 1176 1410
TatI w'GTAC_w
Cuts at: 0 478 591 1069 2586
Size: 478 113 478 1517
Fragments arranged by size:
1517 478 478 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 545 577 615 647 2586
Size: 545 32 38 32 1939
Fragments arranged by size:
1939 545 38 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1054 2586
Size: 1054 1532
XbaI T'CTAG_A
Cuts at: 0 2085 2586
Size: 2085 501
XhoI C'TCGA_G
Cuts at: 0 2558 2586
Size: 2558 28
XmnI GAAnn'nnTTC
Cuts at: 0 1958 2586
Size: 1958 628
Enzymes that do cut:
AcuI AflII AleI AloI AlwNI ApaI ApoI AseI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI
BsrDI BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MfeI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PsrI PvuII SacI
SanDI SexAI SfcI SfoI SgrAI SmlI SpeI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI ApaLI AscI AsiSI BaeI BamHI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
BplI BsaAI BsaBI BsiWI BsmI BspEI BspHI BspMI
BssHII BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MluI MscI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PsiI
PspXI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SfiI SgfI SmaI SnaBI SphI SrfI SspI
SwaI XcmI XmaI ZraI