Restriction map for
pF1KB6277
(Linear) (Six-base) MAPSORT of: KIBB6277.seq Check: 9741 from: 1 to: 2343
KIBB6277.orf
With 182 enzymes: SgfI *
August 6, 2008 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 2031 2343
Size: 1051 980 312
Fragments arranged by size:
1051 980 312
AflIII A'CryG_T
Cuts at: 0 448 2343
Size: 448 1895
AleI CACnn'nnGTG
Cuts at: 0 2162 2231 2343
Size: 2162 69 112
Fragments arranged by size:
2162 112 69
AlwNI CAG_nnn'CTG
Cuts at: 0 393 1171 1224 1969 2343
Size: 393 778 53 745 374
Fragments arranged by size:
778 745 393 374 53
ApaLI G'TGCA_C
Cuts at: 0 1625 2343
Size: 1625 718
ApoI r'AATT_y
Cuts at: 0 874 905 1265 2343
Size: 874 31 360 1078
Fragments arranged by size:
1078 874 360 31
AvaI C'yCGr_G
Cuts at: 0 266 1742 2343
Size: 266 1476 601
Fragments arranged by size:
1476 601 266
BanI G'GyrC_C
Cuts at: 0 112 1591 2101 2343
Size: 112 1479 510 242
Fragments arranged by size:
1479 510 242 112
BanII G_rGCy'C
Cuts at: 0 273 1728 2343
Size: 273 1455 615
Fragments arranged by size:
1455 615 273
BbsI GAAGACnn'nnnn_
Cuts at: 0 1379 2343
Size: 1379 964
BbvCI CC'TCA_GC
Cuts at: 0 1898 2343
Size: 1898 445
BciVI GTATCCnnnnn_n'
Cuts at: 0 165 1629 2343
Size: 165 1464 714
Fragments arranged by size:
1464 714 165
BglII A'GATC_T
Cuts at: 0 2262 2343
Size: 2262 81
Bme1580I G_kGCm'C
Cuts at: 0 1629 2106 2343
Size: 1629 477 237
Fragments arranged by size:
1629 477 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 2285 2343
Size: 722 1563 58
Fragments arranged by size:
1563 722 58
Bpu10I CC'TnA_GC
Cuts at: 0 1865 1898 2343
Size: 1865 33 445
Fragments arranged by size:
1865 445 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 2343
Size: 209 2134
BsaI GGTCTCn'nnnn_
Cuts at: 0 1204 2343
Size: 1204 1139
BsaAI yAC'GTr
Cuts at: 0 451 598 2343
Size: 451 147 1745
Fragments arranged by size:
1745 451 147
BsaWI w'CCGG_w
Cuts at: 0 1720 2343
Size: 1720 623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 111 141 2343
Size: 111 30 2202
Fragments arranged by size:
2202 111 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 563 1904 2343
Size: 563 1341 439
Fragments arranged by size:
1341 563 439
BseYI C'CCAG_C
Cuts at: 0 643 1640 2343
Size: 643 997 703
Fragments arranged by size:
997 703 643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1819 2343
Size: 1277 542 524
Fragments arranged by size:
1277 542 524
BsiHKAI G_wGCw'C
Cuts at: 0 273 1401 1629 2343
Size: 273 1128 228 714
Fragments arranged by size:
1128 714 273 228
BsiWI C'GTAC_G
Cuts at: 0 1648 2343
Size: 1648 695
BsmI GAATG_Cn'
Cuts at: 0 707 1091 1452 2343
Size: 707 384 361 891
Fragments arranged by size:
891 707 384 361
Bsp1286I G_dGCh'C
Cuts at: 0 273 1401 1629 1728 2106 2343
Size: 273 1128 228 99 378 237
Fragments arranged by size:
1128 378 273 237 228 99
BsrDI GCAATG_nn'
Cuts at: 0 266 2343
Size: 266 2077
BsrFI r'CCGG_y
Cuts at: 0 1062 2343
Size: 1062 1281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 442 1570 2343
Size: 442 1128 773
Fragments arranged by size:
1128 773 442
BstBI TT'CG_AA
Cuts at: 0 1542 2343
Size: 1542 801
BstEII G'GTnAC_C
Cuts at: 0 1248 2343
Size: 1248 1095
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1324 1508 2066 2230 2343
Size: 1324 184 558 164 113
Fragments arranged by size:
1324 558 184 164 113
BstYI r'GATC_y
Cuts at: 0 323 1113 2262 2343
Size: 323 790 1149 81
Fragments arranged by size:
1149 790 323 81
Bsu36I CC'TnA_GG
Cuts at: 0 155 2343
Size: 155 2188
BtgI C'CryG_G
Cuts at: 0 38 1655 2343
Size: 38 1617 688
Fragments arranged by size:
1617 688 38
BtsI GCAGTG_nn'
Cuts at: 0 821 2343
Size: 821 1522
EaeI y'GGCC_r
Cuts at: 0 35 686 2172 2220 2343
Size: 35 651 1486 48 123
Fragments arranged by size:
1486 651 123 48 35
EarI CTCTTCn'nnn_
Cuts at: 0 155 1406 2050 2343
Size: 155 1251 644 293
Fragments arranged by size:
1251 644 293 155
EcoICRI GAG'CTC
Cuts at: 0 271 2343
Size: 271 2072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1051 2031 2285 2343
Size: 722 329 980 254 58
Fragments arranged by size:
980 722 329 254 58
EcoO109I rG'GnC_Cy
Cuts at: 0 647 986 1848 2280 2343
Size: 647 339 862 432 63
Fragments arranged by size:
862 647 432 339 63
EcoRI G'AATT_C
Cuts at: 0 1265 2343
Size: 1265 1078
EcoRV GAT'ATC
Cuts at: 0 2125 2343
Size: 2125 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1219 2343
Size: 1219 1124
HaeII r_GCGC'y
Cuts at: 0 1647 2343
Size: 1647 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 408 440 545 577 1182 1214
Size: 255 32 121 32 105 32 605 32
Cuts at: 1214 1530 1562 2025 2057 2067 2099 2343
Size: 316 32 463 32 10 32 244
Fragments arranged by size:
605 463 316 255 244 121 105 32
32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 1123 2343
Size: 1123 1220
HindIII A'AGCT_T
Cuts at: 0 545 965 1091 2343
Size: 545 420 126 1252
Fragments arranged by size:
1252 545 420 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1512 2343
Size: 8 1504 831
Fragments arranged by size:
1504 831 8
MscI TGG'CCA
Cuts at: 0 37 688 2174 2222 2343
Size: 37 651 1486 48 121
Fragments arranged by size:
1486 651 121 48 37
MslI CAynn'nnrTG
Cuts at: 0 37 110 671 2162 2231 2343
Size: 37 73 561 1491 69 112
Fragments arranged by size:
1491 561 112 73 69 37
MspA1I CmG'CkG
Cuts at: 0 643 1261 1803 1966 2319 2343
Size: 643 618 542 163 353 24
Fragments arranged by size:
643 618 542 353 163 24
NaeI GCC'GGC
Cuts at: 0 1064 2343
Size: 1064 1279
NcoI C'CATG_G
Cuts at: 0 38 1655 2343
Size: 38 1617 688
Fragments arranged by size:
1617 688 38
NdeI CA'TA_TG
Cuts at: 0 1959 2343
Size: 1959 384
NgoMIV G'CCGG_C
Cuts at: 0 1062 2343
Size: 1062 1281
NspI r_CATG'y
Cuts at: 0 321 403 2275 2343
Size: 321 82 1872 68
Fragments arranged by size:
1872 321 82 68
PasI CC'CwG_GG
Cuts at: 0 2106 2343
Size: 2106 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 498 2251 2343
Size: 372 126 1753 92
Fragments arranged by size:
1753 372 126 92
PmlI CAC'GTG
Cuts at: 0 451 2343
Size: 451 1892
PpuMI rG'GwC_Cy
Cuts at: 0 647 986 1848 2280 2343
Size: 647 339 862 432 63
Fragments arranged by size:
862 647 432 339 63
PsiI TTA'TAA
Cuts at: 0 1295 2343
Size: 1295 1048
PspXI vC'TCGA_Gb
Cuts at: 0 266 2343
Size: 266 2077
PstI C_TGCA'G
Cuts at: 0 516 839 1845 1884 2343
Size: 516 323 1006 39 459
Fragments arranged by size:
1006 516 459 323 39
PvuII CAG'CTG
Cuts at: 0 1261 1803 1966 2319 2343
Size: 1261 542 163 353 24
Fragments arranged by size:
1261 542 353 163 24
SacI G_AGCT'C
Cuts at: 0 273 2343
Size: 273 2070
SapI GCTCTTCn'nnn_
Cuts at: 0 1406 2343
Size: 1406 937
SexAI A'CCwGG_T
Cuts at: 0 491 2343
Size: 491 1852
SfcI C'TryA_G
Cuts at: 0 512 835 1841 1880 2343
Size: 512 323 1006 39 463
Fragments arranged by size:
1006 512 463 323 39
SmlI C'TyrA_G
Cuts at: 0 8 224 266 2343
Size: 8 216 42 2077
Fragments arranged by size:
2077 216 42 8
SnaBI TAC'GTA
Cuts at: 0 598 2343
Size: 598 1745
SpeI A'CTAG_T
Cuts at: 0 1605 2343
Size: 1605 738
SphI G_CATG'C
Cuts at: 0 321 2275 2343
Size: 321 1954 68
Fragments arranged by size:
1954 321 68
StyI C'CwwG_G
Cuts at: 0 38 1203 1655 2118 2183 2343
Size: 38 1165 452 463 65 160
Fragments arranged by size:
1165 463 452 160 65 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1312 1511 1648 2206 2343
Size: 1312 199 137 558 137
Fragments arranged by size:
1312 558 199 137 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 440 472 1486 1518 1932 1964 2343
Size: 440 32 1014 32 414 32 379
Fragments arranged by size:
1014 440 414 379 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1505 2343
Size: 1505 838
XhoI C'TCGA_G
Cuts at: 0 266 2343
Size: 266 2077
XmnI GAAnn'nnTTC
Cuts at: 0 105 1269 2343
Size: 105 1164 1074
Fragments arranged by size:
1164 1074 105
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BglII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmI Bsp1286I BsrDI BsrFI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PasI PflMI PmlI PpuMI PsiI
PspXI PstI PvuII SacI SapI SexAI SfcI SmlI
SnaBI SpeI SphI StyI TaqII TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI BsaBI BsaHI BsiEI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NarI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PpiI PshAI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SrfI SspI StuI SwaI TatI Tth111I
XbaI XmaI ZraI