Restriction map for
pF1KB6271
(Linear) (Six-base) MAPSORT of: KIBB6271.seq Check: 1995 from: 1 to: 2010
KIBB6271.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 891 1679 1746 2010
Size: 891 788 67 264
Fragments arranged by size:
891 788 264 67
AccI GT'mk_AC
Cuts at: 0 367 2010
Size: 367 1643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 361 397 741 1249 1666 1744 2010
Size: 271 90 36 344 508 417 78 266
Fragments arranged by size:
508 417 344 271 266 90 78 36
AfeI AGC'GCT
Cuts at: 0 807 1336 2010
Size: 807 529 674
Fragments arranged by size:
807 674 529
AgeI A'CCGG_T
Cuts at: 0 1517 2010
Size: 1517 493
AlwNI CAG_nnn'CTG
Cuts at: 0 249 1194 1329 2010
Size: 249 945 135 681
Fragments arranged by size:
945 681 249 135
ApaI G_GGCC'C
Cuts at: 0 114 1493 1538 1825 2010
Size: 114 1379 45 287 185
Fragments arranged by size:
1379 287 185 114 45
ApaLI G'TGCA_C
Cuts at: 0 394 967 2010
Size: 394 573 1043
Fragments arranged by size:
1043 573 394
AscI GG'CGCG_CC
Cuts at: 0 685 2010
Size: 685 1325
AvaI C'yCGr_G
Cuts at: 0 565 1169 1550 2010
Size: 565 604 381 460
Fragments arranged by size:
604 565 460 381
BanI G'GyrC_C
Cuts at: 0 31 412 661 712 1744 2010
Size: 31 381 249 51 1032 266
Fragments arranged by size:
1032 381 266 249 51 31
BanII G_rGCy'C
Cuts at: 0 49 114 272 572 796 1121 1361 1493
Size: 49 65 158 300 224 325 240 132
Cuts at: 1493 1538 1825 2010
Size: 45 287 185
Fragments arranged by size:
325 300 287 240 224 185 158 132
65 49 45
BbeI G_GCGC'C
Cuts at: 0 35 416 1748 2010
Size: 35 381 1332 262
Fragments arranged by size:
1332 381 262 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1131 2010
Size: 1131 879
BglI GCCn_nnn'nGGC
Cuts at: 0 751 892 946 1636 2010
Size: 751 141 54 690 374
Fragments arranged by size:
751 690 374 141 54
BlpI GC'TnA_GC
Cuts at: 0 220 271 2010
Size: 220 51 1739
Fragments arranged by size:
1739 220 51
Bme1580I G_kGCm'C
Cuts at: 0 114 398 624 666 717 971 1493 1538
Size: 114 284 226 42 51 254 522 45
Cuts at: 1538 1825 1962 2010
Size: 287 137 48
Fragments arranged by size:
522 287 284 254 226 137 114 51
48 45 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 608 1957 2010
Size: 608 1349 53
Fragments arranged by size:
1349 608 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 795 891 2010
Size: 405 390 96 1119
Fragments arranged by size:
1119 405 390 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 235 283 463 514 847 1216 1929
Size: 28 207 48 180 51 333 369 713
Cuts at: 1929 1958 2010
Size: 29 52
Fragments arranged by size:
713 369 333 207 180 52 51 48
29 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 340 2010
Size: 340 1670
BsaAI yAC'GTr
Cuts at: 0 1005 1074 1880 2010
Size: 1005 69 806 130
Fragments arranged by size:
1005 806 130 69
BsaHI Gr'CG_yC
Cuts at: 0 32 413 1745 2010
Size: 32 381 1332 265
Fragments arranged by size:
1332 381 265 32
BsaWI w'CCGG_w
Cuts at: 0 971 1517 2010
Size: 971 546 493
Fragments arranged by size:
971 546 493
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 400 430 1039 1069 2010
Size: 217 30 153 30 609 30 941
Fragments arranged by size:
941 609 217 153 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 55 271 280 1149 1213 1267 1568
Size: 25 30 216 9 869 64 54 301
Cuts at: 1568 1891 2010
Size: 323 119
Fragments arranged by size:
869 323 301 216 119 64 54 30
25 9
BseYI C'CCAG_C
Cuts at: 0 472 611 748 914 983 1101 1485 1774
Size: 472 139 137 166 69 118 384 289
Cuts at: 1774 1789 1818 1922 1949 2010
Size: 15 29 104 27 61
Fragments arranged by size:
472 384 289 166 139 137 118 104
69 61 29 27 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1252 2010
Size: 687 565 758
Fragments arranged by size:
758 687 565
BsiEI CG_ry'CG
Cuts at: 0 209 1109 1365 2010
Size: 209 900 256 645
Fragments arranged by size:
900 645 256 209
BsiHKAI G_wGCw'C
Cuts at: 0 272 398 971 1121 1361 2010
Size: 272 126 573 150 240 649
Fragments arranged by size:
649 573 272 240 150 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 196 2010
Size: 196 1814
Bsp1286I G_dGCh'C
Cuts at: 0 49 114 272 398 572 624 666 717
Size: 49 65 158 126 174 52 42 51
Cuts at: 717 796 971 1121 1361 1493 1538 1825 1962
Size: 79 175 150 240 132 45 287 137
Cuts at: 1962 2010
Size: 48
Fragments arranged by size:
287 240 175 174 158 150 137 132
126 79 65 52 51 49 48 45
42
BspHI T'CATG_A
Cuts at: 0 836 2010
Size: 836 1174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 891 1125 1310 1679 1746 2010
Size: 248 643 234 185 369 67 264
Fragments arranged by size:
643 369 264 248 234 185 67
BsrBI CCG'CTC
Cuts at: 0 698 1055 2010
Size: 698 357 955
Fragments arranged by size:
955 698 357
BsrFI r'CCGG_y
Cuts at: 0 34 293 1517 1805 2010
Size: 34 259 1224 288 205
Fragments arranged by size:
1224 288 259 205 34
BssHII G'CGCG_C
Cuts at: 0 538 685 2010
Size: 538 147 1325
Fragments arranged by size:
1325 538 147
BssSI C'ACGA_G
Cuts at: 0 1129 2010
Size: 1129 881
BstAPI GCAn_nnn'nTGC
Cuts at: 0 462 2010
Size: 462 1548
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 1691 2010
Size: 357 1334 319
Fragments arranged by size:
1334 357 319
BstYI r'GATC_y
Cuts at: 0 80 2010
Size: 80 1930
Bsu36I CC'TnA_GG
Cuts at: 0 766 2010
Size: 766 1244
BtgI C'CryG_G
Cuts at: 0 49 890 977 1079 1631 2010
Size: 49 841 87 102 552 379
Fragments arranged by size:
841 552 379 102 87 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1587 2010
Size: 1587 423
DrdI GACnn_nn'nnGTC
Cuts at: 0 1594 2010
Size: 1594 416
EaeI y'GGCC_r
Cuts at: 0 90 291 1343 2005 2010
Size: 90 201 1052 662 5
Fragments arranged by size:
1052 662 201 90 5
EarI CTCTTCn'nnn_
Cuts at: 0 577 655 2010
Size: 577 78 1355
Fragments arranged by size:
1355 577 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1967 2010
Size: 1967 43
EcoICRI GAG'CTC
Cuts at: 0 270 1119 1359 2010
Size: 270 849 240 651
Fragments arranged by size:
849 651 270 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 235 271 283 361 397 463 514
Size: 28 207 36 12 78 36 66 51
Cuts at: 514 741 847 1216 1249 1666 1744 1929 1958
Size: 227 106 369 33 417 78 185 29
Cuts at: 1958 2010
Size: 52
Fragments arranged by size:
417 369 227 207 185 106 78 78
66 52 51 36 36 33 29 28
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 738 928 1246 1291 1453 1648 2010
Size: 454 284 190 318 45 162 195 362
Fragments arranged by size:
454 362 318 284 195 190 162 45
EcoO109I rG'GnC_Cy
Cuts at: 0 110 447 603 752 870 1174 1395 1465
Size: 110 337 156 149 118 304 221 70
Cuts at: 1465 1489 1490 1534 1822 2010
Size: 24 1 44 288 188
Fragments arranged by size:
337 304 288 221 188 156 149 118
110 70 44 24 1
FspI TGC'GCA
Cuts at: 0 822 2010
Size: 822 1188
HaeII r_GCGC'y
Cuts at: 0 35 161 416 809 1261 1338 1748 2010
Size: 35 126 255 393 452 77 410 262
Fragments arranged by size:
452 410 393 262 255 126 77 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 191 223 400 432 2010
Size: 191 32 177 32 1578
Fragments arranged by size:
1578 191 177 32 32
HincII GTy'rAC
Cuts at: 0 231 2010
Size: 231 1779
KasI G'GCGC_C
Cuts at: 0 31 412 1744 2010
Size: 31 381 1332 266
Fragments arranged by size:
1332 381 266 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 2010
Size: 260 1750
MscI TGG'CCA
Cuts at: 0 1345 2010
Size: 1345 665
MslI CAynn'nnrTG
Cuts at: 0 1689 1878 2010
Size: 1689 189 132
Fragments arranged by size:
1689 189 132
MspA1I CmG'CkG
Cuts at: 0 51 96 246 275 375 522 748 918
Size: 51 45 150 29 100 147 226 170
Cuts at: 918 1633 2010
Size: 715 377
Fragments arranged by size:
715 377 226 170 150 147 100 51
45 29
NaeI GCC'GGC
Cuts at: 0 36 295 1807 2010
Size: 36 259 1512 203
Fragments arranged by size:
1512 259 203 36
NarI GG'CG_CC
Cuts at: 0 32 413 1745 2010
Size: 32 381 1332 265
Fragments arranged by size:
1332 381 265 32
NcoI C'CATG_G
Cuts at: 0 890 977 1079 2010
Size: 890 87 102 931
Fragments arranged by size:
931 890 102 87
NgoMIV G'CCGG_C
Cuts at: 0 34 293 1805 2010
Size: 34 259 1512 205
Fragments arranged by size:
1512 259 205 34
NspI r_CATG'y
Cuts at: 0 1036 2010
Size: 1036 974
PasI CC'CwG_GG
Cuts at: 0 333 1455 1826 1999 2010
Size: 333 1122 371 173 11
Fragments arranged by size:
1122 371 333 173 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 1485 2010
Size: 1485 525
PfoI T'CCnGG_A
Cuts at: 0 5 530 1015 2010
Size: 5 525 485 995
Fragments arranged by size:
995 525 485 5
PmlI CAC'GTG
Cuts at: 0 1880 2010
Size: 1880 130
PpuMI rG'GwC_Cy
Cuts at: 0 603 2010
Size: 603 1407
PspOMI G'GGCC_C
Cuts at: 0 110 1489 1534 1821 2010
Size: 110 1379 45 287 189
Fragments arranged by size:
1379 287 189 110 45
PstI C_TGCA'G
Cuts at: 0 59 101 146 245 1148 1334 1757 2010
Size: 59 42 45 99 903 186 423 253
Fragments arranged by size:
903 423 253 186 99 59 45 42
PvuII CAG'CTG
Cuts at: 0 96 246 522 918 2010
Size: 96 150 276 396 1092
Fragments arranged by size:
1092 396 276 150 96
SacI G_AGCT'C
Cuts at: 0 272 1121 1361 2010
Size: 272 849 240 649
Fragments arranged by size:
849 649 272 240
SacII CC_GC'GG
Cuts at: 0 52 1634 2010
Size: 52 1582 376
Fragments arranged by size:
1582 376 52
SanDI GG'GwC_CC
Cuts at: 0 603 2010
Size: 603 1407
SapI GCTCTTCn'nnn_
Cuts at: 0 577 2010
Size: 577 1433
SbfI CC_TGCA'GG
Cuts at: 0 1757 2010
Size: 1757 253
ScaI AGT'ACT
Cuts at: 0 1048 2010
Size: 1048 962
SfcI C'TryA_G
Cuts at: 0 55 97 142 171 241 1144 1330 1753
Size: 55 42 45 29 70 903 186 423
Cuts at: 1753 2010
Size: 257
Fragments arranged by size:
903 423 257 186 70 55 45 42
29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 751 2010
Size: 751 1259
SfoI GGC'GCC
Cuts at: 0 33 414 1746 2010
Size: 33 381 1332 264
Fragments arranged by size:
1332 381 264 33
SgrAI Cr'CCGG_yG
Cuts at: 0 34 2010
Size: 34 1976
StuI AGG'CCT
Cuts at: 0 733 2010
Size: 733 1277
StyI C'CwwG_G
Cuts at: 0 350 890 977 1079 2010
Size: 350 540 87 102 931
Fragments arranged by size:
931 540 350 102 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1123 2010
Size: 1123 887
TatI w'GTAC_w
Cuts at: 0 956 1046 2010
Size: 956 90 964
Fragments arranged by size:
964 956 90
XbaI T'CTAG_A
Cuts at: 0 1780 2010
Size: 1780 230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 676 745 1691 1755 1852 2010
Size: 330 346 69 946 64 97 158
Fragments arranged by size:
946 346 330 158 97 69 64
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmrI BplI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgrAI StuI StyI TaqII TatI
XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I
BpuEI BsaBI BsiWI BsmI BspEI BsrDI BsrGI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraI DraIII EagI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SexAI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XhoI XmaI
XmnI ZraI