Restriction map for
pF1KB6259
(Linear) (Six-base) MAPSORT of: KIBB6259.seq Check: 4906 from: 1 to: 2292
KIBB6259.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 892 2292
Size: 892 1400
AccI GT'mk_AC
Cuts at: 0 293 2292
Size: 293 1999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1970 2292
Size: 1970 322
AfeI AGC'GCT
Cuts at: 0 841 2292
Size: 841 1451
AgeI A'CCGG_T
Cuts at: 0 2084 2292
Size: 2084 208
ApoI r'AATT_y
Cuts at: 0 679 877 1607 1697 2017 2092 2287 2292
Size: 679 198 730 90 320 75 195 5
Fragments arranged by size:
730 679 320 198 195 90 75 5
AvaI C'yCGr_G
Cuts at: 0 1450 2292
Size: 1450 842
BanI G'GyrC_C
Cuts at: 0 1249 1296 2271 2292
Size: 1249 47 975 21
Fragments arranged by size:
1249 975 47 21
BbeI G_GCGC'C
Cuts at: 0 1300 2292
Size: 1300 992
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 772 2292
Size: 93 679 1520
Fragments arranged by size:
1520 679 93
BciVI GTATCCnnnnn_n'
Cuts at: 0 825 918 2292
Size: 825 93 1374
Fragments arranged by size:
1374 825 93
BfrBI ATG'CAT
Cuts at: 0 322 2292
Size: 322 1970
Bme1580I G_kGCm'C
Cuts at: 0 866 2292
Size: 866 1426
BmgBI CAC'GTC
Cuts at: 0 996 2292
Size: 996 1296
BmrI ACTGGGnnnn_n'
Cuts at: 0 298 1147 2192 2292
Size: 298 849 1045 100
Fragments arranged by size:
1045 849 298 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1176 2292
Size: 1176 1116
BsaAI yAC'GTr
Cuts at: 0 2254 2292
Size: 2254 38
BsaBI GATnn'nnATC
Cuts at: 0 1112 1620 2292
Size: 1112 508 672
Fragments arranged by size:
1112 672 508
BsaHI Gr'CG_yC
Cuts at: 0 1297 2292
Size: 1297 995
BsaWI w'CCGG_w
Cuts at: 0 2084 2292
Size: 2084 208
BseYI C'CCAG_C
Cuts at: 0 333 1174 1237 2292
Size: 333 841 63 1055
Fragments arranged by size:
1055 841 333 63
BsiHKAI G_wGCw'C
Cuts at: 0 1216 2292
Size: 1216 1076
BsmI GAATG_Cn'
Cuts at: 0 1355 2292
Size: 1355 937
Bsp1286I G_dGCh'C
Cuts at: 0 866 1216 2292
Size: 866 350 1076
Fragments arranged by size:
1076 866 350
BspHI T'CATG_A
Cuts at: 0 1418 2292
Size: 1418 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 892 1880 2292
Size: 892 988 412
Fragments arranged by size:
988 892 412
BsrDI GCAATG_nn'
Cuts at: 0 462 1046 2292
Size: 462 584 1246
Fragments arranged by size:
1246 584 462
BsrFI r'CCGG_y
Cuts at: 0 200 2084 2292
Size: 200 1884 208
Fragments arranged by size:
1884 208 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1045 1755 2292
Size: 1045 710 537
Fragments arranged by size:
1045 710 537
BstBI TT'CG_AA
Cuts at: 0 1695 2292
Size: 1695 597
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1443 2292
Size: 1443 849
BtgI C'CryG_G
Cuts at: 0 377 2292
Size: 377 1915
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1020 2292
Size: 1020 1272
BtsI GCAGTG_nn'
Cuts at: 0 1061 1970 2292
Size: 1061 909 322
Fragments arranged by size:
1061 909 322
ClaI AT'CG_AT
Cuts at: 0 1670 2005 2292
Size: 1670 335 287
Fragments arranged by size:
1670 335 287
DraI TTT'AAA
Cuts at: 0 927 939 2292
Size: 927 12 1353
Fragments arranged by size:
1353 927 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 702 2292
Size: 702 1590
EaeI y'GGCC_r
Cuts at: 0 32 257 2292
Size: 32 225 2035
Fragments arranged by size:
2035 225 32
EarI CTCTTCn'nnn_
Cuts at: 0 1811 2292
Size: 1811 481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1674 2292
Size: 1674 618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1176 1970 2292
Size: 1176 794 322
Fragments arranged by size:
1176 794 322
EcoO109I rG'GnC_Cy
Cuts at: 0 1505 1920 1995 2292
Size: 1505 415 75 297
Fragments arranged by size:
1505 415 297 75
EcoRI G'AATT_C
Cuts at: 0 877 1697 2292
Size: 877 820 595
Fragments arranged by size:
877 820 595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 871 1770 2292
Size: 871 899 522
Fragments arranged by size:
899 871 522
HaeII r_GCGC'y
Cuts at: 0 843 1300 2292
Size: 843 457 992
Fragments arranged by size:
992 843 457
HincII GTy'rAC
Cuts at: 0 294 695 1794 2292
Size: 294 401 1099 498
Fragments arranged by size:
1099 498 401 294
HindIII A'AGCT_T
Cuts at: 0 1718 2077 2292
Size: 1718 359 215
Fragments arranged by size:
1718 359 215
KasI G'GCGC_C
Cuts at: 0 1296 2292
Size: 1296 996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2031 2292
Size: 2031 261
MscI TGG'CCA
Cuts at: 0 34 2292
Size: 34 2258
MslI CAynn'nnrTG
Cuts at: 0 376 1039 1258 2292
Size: 376 663 219 1034
Fragments arranged by size:
1034 663 376 219
NarI GG'CG_CC
Cuts at: 0 1297 2292
Size: 1297 995
NsiI A_TGCA'T
Cuts at: 0 324 2292
Size: 324 1968
PflMI CCAn_nnn'nTGG
Cuts at: 0 310 1108 2292
Size: 310 798 1184
Fragments arranged by size:
1184 798 310
PfoI T'CCnGG_A
Cuts at: 0 966 2292
Size: 966 1326
PpuMI rG'GwC_Cy
Cuts at: 0 1505 1920 2292
Size: 1505 415 372
Fragments arranged by size:
1505 415 372
PstI C_TGCA'G
Cuts at: 0 1745 2292
Size: 1745 547
SalI G'TCGA_C
Cuts at: 0 292 2292
Size: 292 2000
SapI GCTCTTCn'nnn_
Cuts at: 0 1811 2292
Size: 1811 481
ScaI AGT'ACT
Cuts at: 0 737 1182 1844 2181 2292
Size: 737 445 662 337 111
Fragments arranged by size:
737 662 445 337 111
SexAI A'CCwGG_T
Cuts at: 0 782 2292
Size: 782 1510
SfcI C'TryA_G
Cuts at: 0 1741 2292
Size: 1741 551
SfoI GGC'GCC
Cuts at: 0 1298 2292
Size: 1298 994
SnaBI TAC'GTA
Cuts at: 0 2254 2292
Size: 2254 38
SspI AAT'ATT
Cuts at: 0 774 1539 2123 2292
Size: 774 765 584 169
Fragments arranged by size:
774 765 584 169
StuI AGG'CCT
Cuts at: 0 1460 2292
Size: 1460 832
StyI C'CwwG_G
Cuts at: 0 1130 2292
Size: 1130 1162
TatI w'GTAC_w
Cuts at: 0 735 1180 1786 1842 2179 2292
Size: 735 445 606 56 337 113
Fragments arranged by size:
735 606 445 337 113 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 587 619 2292
Size: 587 32 1673
Fragments arranged by size:
1673 587 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 2292
Size: 574 1718
XmnI GAAnn'nnTTC
Cuts at: 0 1609 2292
Size: 1609 683
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI ApoI AvaI BanI
BbeI BbsI BciVI BfrBI Bme1580I BmgBI BmrI BpmI
BsaAI BsaBI BsaHI BsaWI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BstAPI BstBI BstXI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI FalI HaeII HincII HindIII KasI
MmeI MscI MslI NarI NsiI PflMI PfoI PpuMI
PstI SalI SapI ScaI SexAI SfcI SfoI SnaBI
SspI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbvCI BcgI BclI BglI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaI BsaXI BseRI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstEII BstYI BstZ17I Bsu36I CspCI DraIII EagI
EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I HpaI
KpnI MfeI MluI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SanDI SbfI SfiI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI