Restriction map for
pF1KB6256
(Linear) (Six-base) MAPSORT of: KIBB6256.seq Check: 1327 from: 1 to: 2049
KIBB6256.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 0 1231 2049
Size: 1231 818
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 943 1331 1981 2049
Size: 71 872 388 650 68
Fragments arranged by size:
872 650 388 71 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1279 2049
Size: 1279 770
AlwNI CAG_nnn'CTG
Cuts at: 0 442 633 898 1152 1761 2049
Size: 442 191 265 254 609 288
Fragments arranged by size:
609 442 288 265 254 191
ApaI G_GGCC'C
Cuts at: 0 1519 2049
Size: 1519 530
ApoI r'AATT_y
Cuts at: 0 1432 2049
Size: 1432 617
BanI G'GyrC_C
Cuts at: 0 606 708 1457 2049
Size: 606 102 749 592
Fragments arranged by size:
749 606 592 102
BanII G_rGCy'C
Cuts at: 0 110 784 916 1519 2021 2049
Size: 110 674 132 603 502 28
Fragments arranged by size:
674 603 502 132 110 28
BbeI G_GCGC'C
Cuts at: 0 610 712 1461 2049
Size: 610 102 749 588
Fragments arranged by size:
749 610 588 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 949 2049
Size: 949 1100
BbvCI CC'TCA_GC
Cuts at: 0 713 1724 1730 2049
Size: 713 1011 6 319
Fragments arranged by size:
1011 713 319 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 144 178 2049
Size: 144 34 1871
Fragments arranged by size:
1871 144 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1655 2049
Size: 1655 394
BclI T'GATC_A
Cuts at: 0 1160 2049
Size: 1160 889
BglI GCCn_nnn'nGGC
Cuts at: 0 604 2049
Size: 604 1445
Bme1580I G_kGCm'C
Cuts at: 0 1519 1689 2049
Size: 1519 170 360
Fragments arranged by size:
1519 360 170
BmtI G_CTAG'C
Cuts at: 0 1610 2049
Size: 1610 439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 505 511 517 768 797 936 1438
Size: 496 9 6 6 251 29 139 502
Cuts at: 1438 2049
Size: 611
Fragments arranged by size:
611 502 496 251 139 29 9 6
6
Bpu10I CC'TnA_GC
Cuts at: 0 713 1724 1730 2049
Size: 713 1011 6 319
Fragments arranged by size:
1011 713 319 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1729 1852 2049
Size: 1729 123 197
Fragments arranged by size:
1729 197 123
BsaHI Gr'CG_yC
Cuts at: 0 607 709 1228 1458 2049
Size: 607 102 519 230 591
Fragments arranged by size:
607 591 519 230 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 1204 1234 2049
Size: 727 30 447 30 815
Fragments arranged by size:
815 727 447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1090 1320 1329 1435 2049
Size: 1090 230 9 106 614
Fragments arranged by size:
1090 614 230 106 9
BseYI C'CCAG_C
Cuts at: 0 151 180 318 506 620 650 719 773
Size: 151 29 138 188 114 30 69 54
Cuts at: 773 1094 2049
Size: 321 955
Fragments arranged by size:
955 321 188 151 138 114 69 54
30 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1249 2049
Size: 1099 150 800
Fragments arranged by size:
1099 800 150
BsiHKAI G_wGCw'C
Cuts at: 0 784 916 2049
Size: 784 132 1133
Fragments arranged by size:
1133 784 132
BsmI GAATG_Cn'
Cuts at: 0 57 2049
Size: 57 1992
Bsp1286I G_dGCh'C
Cuts at: 0 110 784 916 1519 1689 2021 2049
Size: 110 674 132 603 170 332 28
Fragments arranged by size:
674 603 332 170 132 110 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1738 2049
Size: 1738 311
BsrBI CCG'CTC
Cuts at: 0 837 1713 2049
Size: 837 876 336
Fragments arranged by size:
876 837 336
BsrDI GCAATG_nn'
Cuts at: 0 948 2049
Size: 948 1101
BsrFI r'CCGG_y
Cuts at: 0 295 1701 1995 2049
Size: 295 1406 294 54
Fragments arranged by size:
1406 295 294 54
BssSI C'ACGA_G
Cuts at: 0 1990 2049
Size: 1990 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 442 633 898 2049
Size: 442 191 265 1151
Fragments arranged by size:
1151 442 265 191
BstYI r'GATC_y
Cuts at: 0 1148 1424 1835 2049
Size: 1148 276 411 214
Fragments arranged by size:
1148 411 276 214
BtgI C'CryG_G
Cuts at: 0 2000 2049
Size: 2000 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1110 2049
Size: 1110 939
BtsI GCAGTG_nn'
Cuts at: 0 394 469 2049
Size: 394 75 1580
Fragments arranged by size:
1580 394 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1119 1154 2049
Size: 1119 35 895
Fragments arranged by size:
1119 895 35
DraI TTT'AAA
Cuts at: 0 1449 2049
Size: 1449 600
EaeI y'GGCC_r
Cuts at: 0 293 1200 1535 1997 2049
Size: 293 907 335 462 52
Fragments arranged by size:
907 462 335 293 52
EarI CTCTTCn'nnn_
Cuts at: 0 166 1326 1347 2049
Size: 166 1160 21 702
Fragments arranged by size:
1160 702 166 21
EcoICRI GAG'CTC
Cuts at: 0 782 914 2049
Size: 782 132 1135
Fragments arranged by size:
1135 782 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 496 505 511 517 768 797 936
Size: 71 425 9 6 6 251 29 139
Cuts at: 936 943 1331 1438 1981 2049
Size: 7 388 107 543 68
Fragments arranged by size:
543 425 388 251 139 107 71 68
29 9 7 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1031 1579 2049
Size: 1031 548 470
Fragments arranged by size:
1031 548 470
EcoO109I rG'GnC_Cy
Cuts at: 0 313 1515 1516 1827 2049
Size: 313 1202 1 311 222
Fragments arranged by size:
1202 313 311 222 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1276 2049
Size: 1276 773
HaeII r_GCGC'y
Cuts at: 0 610 712 1461 2049
Size: 610 102 749 588
Fragments arranged by size:
749 610 588 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 406 989 1014 1021 1046 1467 1484
Size: 374 32 583 25 7 25 421 17
Cuts at: 1484 1499 1516 1538 1570 2049
Size: 15 17 22 32 479
Fragments arranged by size:
583 479 421 374 32 32 25 25
22 17 17 15 7
HincII GTy'rAC
Cuts at: 0 575 976 2049
Size: 575 401 1073
Fragments arranged by size:
1073 575 401
HindIII A'AGCT_T
Cuts at: 0 1442 2049
Size: 1442 607
KasI G'GCGC_C
Cuts at: 0 606 708 1457 2049
Size: 606 102 749 592
Fragments arranged by size:
749 606 592 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 2049
Size: 793 1256
MspA1I CmG'CkG
Cuts at: 0 442 633 699 861 895 1393 2049
Size: 442 191 66 162 34 498 656
Fragments arranged by size:
656 498 442 191 162 66 34
NaeI GCC'GGC
Cuts at: 0 297 1997 2049
Size: 297 1700 52
Fragments arranged by size:
1700 297 52
NarI GG'CG_CC
Cuts at: 0 607 709 1458 2049
Size: 607 102 749 591
Fragments arranged by size:
749 607 591 102
NgoMIV G'CCGG_C
Cuts at: 0 295 1995 2049
Size: 295 1700 54
Fragments arranged by size:
1700 295 54
NheI G'CTAG_C
Cuts at: 0 1606 2049
Size: 1606 443
PasI CC'CwG_GG
Cuts at: 0 1113 1170 2049
Size: 1113 57 879
Fragments arranged by size:
1113 879 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 820 2049
Size: 429 391 1229
Fragments arranged by size:
1229 429 391
PfoI T'CCnGG_A
Cuts at: 0 813 2049
Size: 813 1236
PspOMI G'GGCC_C
Cuts at: 0 1515 2049
Size: 1515 534
PstI C_TGCA'G
Cuts at: 0 447 638 894 903 1546 2049
Size: 447 191 256 9 643 503
Fragments arranged by size:
643 503 447 256 191 9
PvuII CAG'CTG
Cuts at: 0 442 699 861 2049
Size: 442 257 162 1188
Fragments arranged by size:
1188 442 257 162
SacI G_AGCT'C
Cuts at: 0 784 916 2049
Size: 784 132 1133
Fragments arranged by size:
1133 784 132
SapI GCTCTTCn'nnn_
Cuts at: 0 166 2049
Size: 166 1883
SfcI C'TryA_G
Cuts at: 0 443 634 856 890 899 1508 1542 1601
Size: 443 191 222 34 9 609 34 59
Cuts at: 1601 2049
Size: 448
Fragments arranged by size:
609 448 443 222 191 59 34 34
9
SfoI GGC'GCC
Cuts at: 0 608 710 1459 2049
Size: 608 102 749 590
Fragments arranged by size:
749 608 590 102
SmlI C'TyrA_G
Cuts at: 0 1708 1831 2049
Size: 1708 123 218
Fragments arranged by size:
1708 218 123
SpeI A'CTAG_T
Cuts at: 0 978 2049
Size: 978 1071
StuI AGG'CCT
Cuts at: 0 934 2049
Size: 934 1115
StyI C'CwwG_G
Cuts at: 0 423 1251 1467 2049
Size: 423 828 216 582
Fragments arranged by size:
828 582 423 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 861 1183 1203 1780 1903 1931 2049
Size: 861 322 20 577 123 28 118
Fragments arranged by size:
861 577 322 123 118 28 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 1204 1236 2049
Size: 727 32 445 32 813
Fragments arranged by size:
813 727 445 32 32
ZraI GAC'GTC
Cuts at: 0 1229 2049
Size: 1229 820
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI ApoI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI Bme1580I
BmtI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstYI BtgI BtgZI BtsI CspCI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII KasI MmeI MspA1I NaeI NarI
NgoMIV NheI PasI PflMI PfoI PspOMI PstI PvuII
SacI SapI SfcI SfoI SmlI SpeI StuI StyI
TaqII TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BfrBI BglII BlpI BmgBI BmrI
BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I ClaI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspI FspAI HpaI KpnI MfeI MluI MscI
MslI NcoI NdeI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI