Restriction map for
pF1KB6246
(Linear) (Six-base) MAPSORT of: KIBB6246.seq Check: 7145 from: 1 to: 2040
KIBB6246.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 470 587 1029 1115 1896 2040
Size: 288 182 117 442 86 781 144
Fragments arranged by size:
781 442 288 182 144 117 86
AatII G_ACGT'C
Cuts at: 0 1397 2040
Size: 1397 643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1122 2040
Size: 1122 918
AleI CACnn'nnGTG
Cuts at: 0 1669 2040
Size: 1669 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 681 1816 2040
Size: 681 1135 224
Fragments arranged by size:
1135 681 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1768 1800 2040
Size: 1768 32 240
Fragments arranged by size:
1768 240 32
AlwNI CAG_nnn'CTG
Cuts at: 0 228 363 549 579 603 1053 1149 1252
Size: 228 135 186 30 24 450 96 103
Cuts at: 1252 2040
Size: 788
Fragments arranged by size:
788 450 228 186 135 103 96 30
24
ApaI G_GGCC'C
Cuts at: 0 638 1309 2040
Size: 638 671 731
Fragments arranged by size:
731 671 638
ApaLI G'TGCA_C
Cuts at: 0 1225 2040
Size: 1225 815
AvaI C'yCGr_G
Cuts at: 0 630 709 1948 1983 2040
Size: 630 79 1239 35 57
Fragments arranged by size:
1239 630 79 57 35
BanI G'GyrC_C
Cuts at: 0 300 703 1597 1825 1963 2040
Size: 300 403 894 228 138 77
Fragments arranged by size:
894 403 300 228 138 77
BanII G_rGCy'C
Cuts at: 0 256 638 1130 1285 1309 1663 2040
Size: 256 382 492 155 24 354 377
Fragments arranged by size:
492 382 377 354 256 155 24
BbeI G_GCGC'C
Cuts at: 0 1967 2040
Size: 1967 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 1943 2003 2040
Size: 962 981 60 37
Fragments arranged by size:
981 962 60 37
BbvCI CC'TCA_GC
Cuts at: 0 1555 1693 1807 2040
Size: 1555 138 114 233
Fragments arranged by size:
1555 233 138 114
BglI GCCn_nnn'nGGC
Cuts at: 0 1099 1732 2040
Size: 1099 633 308
Fragments arranged by size:
1099 633 308
BglII A'GATC_T
Cuts at: 0 1475 2040
Size: 1475 565
Bme1580I G_kGCm'C
Cuts at: 0 638 708 1229 1309 1830 2040
Size: 638 70 521 80 521 210
Fragments arranged by size:
638 521 521 210 80 70
BmgBI CAC'GTC
Cuts at: 0 1821 2040
Size: 1821 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 523 1014 1881 2040
Size: 523 491 867 159
Fragments arranged by size:
867 523 491 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 400 625 1075 2029 2040
Size: 376 24 225 450 954 11
Fragments arranged by size:
954 450 376 225 24 11
Bpu10I CC'TnA_GC
Cuts at: 0 250 1555 1693 1807 2040
Size: 250 1305 138 114 233
Fragments arranged by size:
1305 250 233 138 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 807 880 937 1225 1632 2040
Size: 807 73 57 288 407 408
Fragments arranged by size:
807 408 407 288 73 57
BsaHI Gr'CG_yC
Cuts at: 0 1394 1964 2040
Size: 1394 570 76
Fragments arranged by size:
1394 570 76
BsaWI w'CCGG_w
Cuts at: 0 1007 1992 2040
Size: 1007 985 48
Fragments arranged by size:
1007 985 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 1311 1341 1768 1798 2040
Size: 191 30 1090 30 427 30 242
Fragments arranged by size:
1090 427 242 191 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 898 1158 1161 1341 1744 1859 1930
Size: 460 438 260 3 180 403 115 71
Cuts at: 1930 2026 2029 2040
Size: 96 3 11
Fragments arranged by size:
460 438 403 260 180 115 96 71
11 3 3
BseYI C'CCAG_C
Cuts at: 0 476 487 683 1308 1846 1975 2040
Size: 476 11 196 625 538 129 65
Fragments arranged by size:
625 538 476 196 129 65 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 169 316 1066 1093 1546 1924 2040
Size: 167 2 147 750 27 453 378 116
Fragments arranged by size:
750 453 378 167 147 116 27 2
BsiEI CG_ry'CG
Cuts at: 0 767 1244 2040
Size: 767 477 796
Fragments arranged by size:
796 767 477
BsiHKAI G_wGCw'C
Cuts at: 0 256 854 995 1229 1707 2040
Size: 256 598 141 234 478 333
Fragments arranged by size:
598 478 333 256 234 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1197 2040
Size: 1197 843
Bsp1286I G_dGCh'C
Cuts at: 0 256 638 708 854 995 1130 1229 1285
Size: 256 382 70 146 141 135 99 56
Cuts at: 1285 1309 1663 1707 1830 2040
Size: 24 354 44 123 210
Fragments arranged by size:
382 354 256 210 146 141 135 123
99 70 56 44 24
BspEI T'CCGG_A
Cuts at: 0 1992 2040
Size: 1992 48
BspHI T'CATG_A
Cuts at: 0 1371 2040
Size: 1371 669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 470 587 596 686 1029 1115 1896
Size: 288 182 117 9 90 343 86 781
Cuts at: 1896 2040
Size: 144
Fragments arranged by size:
781 343 288 182 144 117 90 86
9
BsrBI CCG'CTC
Cuts at: 0 790 1083 1620 2040
Size: 790 293 537 420
Fragments arranged by size:
790 537 420 293
BsrDI GCAATG_nn'
Cuts at: 0 162 2040
Size: 162 1878
BssSI C'ACGA_G
Cuts at: 0 880 1222 2040
Size: 880 342 818
Fragments arranged by size:
880 818 342
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 351 387 549 564 579 1044 1711
Size: 228 123 36 162 15 15 465 667
Cuts at: 1711 2040
Size: 329
Fragments arranged by size:
667 465 329 228 162 123 36 15
15
BstEII G'GTnAC_C
Cuts at: 0 1185 2040
Size: 1185 855
BstYI r'GATC_y
Cuts at: 0 359 1058 1475 2040
Size: 359 699 417 565
Fragments arranged by size:
699 565 417 359
Bsu36I CC'TnA_GG
Cuts at: 0 243 2040
Size: 243 1797
BtgI C'CryG_G
Cuts at: 0 140 1832 2040
Size: 140 1692 208
Fragments arranged by size:
1692 208 140
BtsI GCAGTG_nn'
Cuts at: 0 1332 1520 2040
Size: 1332 188 520
Fragments arranged by size:
1332 520 188
DraIII CAC_nnn'GTG
Cuts at: 0 1902 2040
Size: 1902 138
DrdI GACnn_nn'nnGTC
Cuts at: 0 30 2040
Size: 30 2010
EaeI y'GGCC_r
Cuts at: 0 314 764 931 1241 1733 2040
Size: 314 450 167 310 492 307
Fragments arranged by size:
492 450 314 310 307 167
EagI C'GGCC_G
Cuts at: 0 764 1241 2040
Size: 764 477 799
Fragments arranged by size:
799 764 477
EarI CTCTTCn'nnn_
Cuts at: 0 879 2040
Size: 879 1161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1417 1776 2040
Size: 1417 359 264
Fragments arranged by size:
1417 359 264
EcoICRI GAG'CTC
Cuts at: 0 254 2040
Size: 254 1786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 400 625 1075 1122 2029 2040
Size: 376 24 225 450 47 907 11
Fragments arranged by size:
907 450 376 225 47 24 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2011 2040
Size: 2011 29
EcoO109I rG'GnC_Cy
Cuts at: 0 634 635 1305 1987 2004 2040
Size: 634 1 670 682 17 36
Fragments arranged by size:
682 670 634 36 17 1
HaeII r_GCGC'y
Cuts at: 0 1967 1982 2040
Size: 1967 15 58
Fragments arranged by size:
1967 58 15
KasI G'GCGC_C
Cuts at: 0 1963 2040
Size: 1963 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 2040
Size: 1297 743
MscI TGG'CCA
Cuts at: 0 316 933 2040
Size: 316 617 1107
Fragments arranged by size:
1107 617 316
MslI CAynn'nnrTG
Cuts at: 0 1495 1669 2040
Size: 1495 174 371
Fragments arranged by size:
1495 371 174
MspA1I CmG'CkG
Cuts at: 0 92 378 528 661 687 1053 1461 1842
Size: 92 286 150 133 26 366 408 381
Cuts at: 1842 2040
Size: 198
Fragments arranged by size:
408 381 366 286 198 150 133 92
26
NarI GG'CG_CC
Cuts at: 0 1964 2040
Size: 1964 76
NspI r_CATG'y
Cuts at: 0 1116 1773 2040
Size: 1116 657 267
Fragments arranged by size:
1116 657 267
PasI CC'CwG_GG
Cuts at: 0 259 1959 2040
Size: 259 1700 81
Fragments arranged by size:
1700 259 81
PfoI T'CCnGG_A
Cuts at: 0 1439 2040
Size: 1439 601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1669 1701 1768 1800 2040
Size: 1669 32 67 32 240
Fragments arranged by size:
1669 240 67 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2004 2040
Size: 2004 36
PspOMI G'GGCC_C
Cuts at: 0 634 1305 2040
Size: 634 671 735
Fragments arranged by size:
735 671 634
PstI C_TGCA'G
Cuts at: 0 236 383 467 593 617 1091 1200 2040
Size: 236 147 84 126 24 474 109 840
Fragments arranged by size:
840 474 236 147 126 109 84 24
PvuII CAG'CTG
Cuts at: 0 378 528 661 687 1053 1461 2040
Size: 378 150 133 26 366 408 579
Fragments arranged by size:
579 408 378 366 150 133 26
SacI G_AGCT'C
Cuts at: 0 256 2040
Size: 256 1784
SapI GCTCTTCn'nnn_
Cuts at: 0 879 2040
Size: 879 1161
ScaI AGT'ACT
Cuts at: 0 433 2040
Size: 433 1607
SexAI A'CCwGG_T
Cuts at: 0 1898 2040
Size: 1898 142
SfcI C'TryA_G
Cuts at: 0 232 379 424 463 589 613 1087 1196
Size: 232 147 45 39 126 24 474 109
Cuts at: 1196 2040
Size: 844
Fragments arranged by size:
844 474 232 147 126 109 45 39
24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1099 1732 2040
Size: 1099 633 308
Fragments arranged by size:
1099 633 308
SfoI GGC'GCC
Cuts at: 0 1965 2040
Size: 1965 75
SmaI CCC'GGG
Cuts at: 0 711 1950 2040
Size: 711 1239 90
Fragments arranged by size:
1239 711 90
SphI G_CATG'C
Cuts at: 0 1773 2040
Size: 1773 267
SrfI GCCC'GGGC
Cuts at: 0 1950 2040
Size: 1950 90
StuI AGG'CCT
Cuts at: 0 241 248 385 2040
Size: 241 7 137 1655
Fragments arranged by size:
1655 241 137 7
StyI C'CwwG_G
Cuts at: 0 797 1070 1181 2040
Size: 797 273 111 859
Fragments arranged by size:
859 797 273 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 490 1813 2040
Size: 490 1323 227
Fragments arranged by size:
1323 490 227
TatI w'GTAC_w
Cuts at: 0 431 1012 1889 2040
Size: 431 581 877 151
Fragments arranged by size:
877 581 431 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1311 1343 2040
Size: 1311 32 697
Fragments arranged by size:
1311 697 32
Tth111I GACn'n_nGTC
Cuts at: 0 730 2040
Size: 730 1310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 484 510 2040
Size: 484 26 1530
Fragments arranged by size:
1530 484 26
XmaI C'CCGG_G
Cuts at: 0 709 1948 2040
Size: 709 1239 92
Fragments arranged by size:
1239 709 92
ZraI GAC'GTC
Cuts at: 0 1395 2040
Size: 1395 645
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
KasI MmeI MscI MslI MspA1I NarI NspI PasI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BlpI BmtI BplI BpuEI BsaAI
BsaBI BsiWI BsmI BsrFI BsrGI BssHII BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI