Restriction map for
pF1KB6238
(Linear) (Six-base) MAPSORT of: KIBB6238.seq Check: 7197 from: 1 to: 2031
KIBB6238.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 386 2031
Size: 386 1645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 497 514 553 643 1540 2031
Size: 40 457 17 39 90 897 491
Fragments arranged by size:
897 491 457 90 40 39 17
AfeI AGC'GCT
Cuts at: 0 863 2031
Size: 863 1168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 752 2031
Size: 52 700 1279
Fragments arranged by size:
1279 700 52
AlwNI CAG_nnn'CTG
Cuts at: 0 765 1557 1659 2031
Size: 765 792 102 372
Fragments arranged by size:
792 765 372 102
ApaI G_GGCC'C
Cuts at: 0 612 1370 1404 2031
Size: 612 758 34 627
Fragments arranged by size:
758 627 612 34
ApoI r'AATT_y
Cuts at: 0 1351 2031
Size: 1351 680
AvaI C'yCGr_G
Cuts at: 0 368 436 1397 2031
Size: 368 68 961 634
Fragments arranged by size:
961 634 368 68
BamHI G'GATC_C
Cuts at: 0 1205 2031
Size: 1205 826
BanI G'GyrC_C
Cuts at: 0 372 588 2031
Size: 372 216 1443
Fragments arranged by size:
1443 372 216
BanII G_rGCy'C
Cuts at: 0 612 700 1370 1404 2031
Size: 612 88 670 34 627
Fragments arranged by size:
670 627 612 88 34
BbeI G_GCGC'C
Cuts at: 0 592 2031
Size: 592 1439
BbsI GAAGACnn'nnnn_
Cuts at: 0 527 1105 2031
Size: 527 578 926
Fragments arranged by size:
926 578 527
BbvCI CC'TCA_GC
Cuts at: 0 5 1666 2018 2031
Size: 5 1661 352 13
Fragments arranged by size:
1661 352 13 5
BglI GCCn_nnn'nGGC
Cuts at: 0 715 1345 2031
Size: 715 630 686
Fragments arranged by size:
715 686 630
BglII A'GATC_T
Cuts at: 0 1016 2031
Size: 1016 1015
BlpI GC'TnA_GC
Cuts at: 0 25 406 1142 1234 1421 2031
Size: 25 381 736 92 187 610
Fragments arranged by size:
736 610 381 187 92 25
Bme1580I G_kGCm'C
Cuts at: 0 377 612 984 1370 1404 1926 2031
Size: 377 235 372 386 34 522 105
Fragments arranged by size:
522 386 377 372 235 105 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 987 2031
Size: 987 1044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1617 1862 1949 1955 2031
Size: 622 995 245 87 6 76
Fragments arranged by size:
995 622 245 87 76 6
Bpu10I CC'TnA_GC
Cuts at: 0 5 1666 1686 2018 2031
Size: 5 1661 20 332 13
Fragments arranged by size:
1661 332 20 13 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 1758 1830 2031
Size: 94 1664 72 201
Fragments arranged by size:
1664 201 94 72
BsaHI Gr'CG_yC
Cuts at: 0 383 589 1102 1337 2031
Size: 383 206 513 235 694
Fragments arranged by size:
694 513 383 235 206
BsaWI w'CCGG_w
Cuts at: 0 1247 2031
Size: 1247 784
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1996 2026 2031
Size: 1996 30 5
Fragments arranged by size:
1996 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 112 337 1363 1589 2031
Size: 22 90 225 1026 226 442
Fragments arranged by size:
1026 442 226 225 90 22
BseYI C'CCAG_C
Cuts at: 0 450 1085 1146 1649 1726 1777 1948 1997
Size: 450 635 61 503 77 51 171 49
Cuts at: 1997 2031
Size: 34
Fragments arranged by size:
635 503 450 171 77 61 51 49
34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1550 1753 2031
Size: 1288 262 203 278
Fragments arranged by size:
1288 278 262 203
BsiHKAI G_wGCw'C
Cuts at: 0 1296 2031
Size: 1296 735
Bsp1286I G_dGCh'C
Cuts at: 0 377 612 700 984 1296 1370 1404 1926
Size: 377 235 88 284 312 74 34 522
Cuts at: 1926 2031
Size: 105
Fragments arranged by size:
522 377 312 284 235 105 88 74
34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 937 1416 1644 2031
Size: 112 825 479 228 387
Fragments arranged by size:
825 479 387 228 112
BsrBI CCG'CTC
Cuts at: 0 1203 1573 2031
Size: 1203 370 458
Fragments arranged by size:
1203 458 370
BsrDI GCAATG_nn'
Cuts at: 0 734 2031
Size: 734 1297
BssSI C'ACGA_G
Cuts at: 0 640 1568 2031
Size: 640 928 463
Fragments arranged by size:
928 640 463
BstAPI GCAn_nnn'nTGC
Cuts at: 0 157 2031
Size: 157 1874
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 2031
Size: 60 1971
BstYI r'GATC_y
Cuts at: 0 1016 1205 2031
Size: 1016 189 826
Fragments arranged by size:
1016 826 189
BtgI C'CryG_G
Cuts at: 0 74 740 769 2031
Size: 74 666 29 1262
Fragments arranged by size:
1262 666 74 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 66 1113 1521 2031
Size: 66 1047 408 510
Fragments arranged by size:
1047 510 408 66
BtsI GCAGTG_nn'
Cuts at: 0 1218 1781 1926 2031
Size: 1218 563 145 105
Fragments arranged by size:
1218 563 145 105
DrdI GACnn_nn'nnGTC
Cuts at: 0 1560 2031
Size: 1560 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 2031
Size: 733 1298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 497 514 553 622 643 1540 1617
Size: 40 457 17 39 69 21 897 77
Cuts at: 1617 1862 1949 1955 2031
Size: 245 87 6 76
Fragments arranged by size:
897 457 245 87 77 76 69 40
39 21 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 2031
Size: 1480 551
EcoO109I rG'GnC_Cy
Cuts at: 0 608 609 1358 1367 1485 1656 1858 2031
Size: 608 1 749 9 118 171 202 173
Fragments arranged by size:
749 608 202 173 171 118 9 1
EcoRI G'AATT_C
Cuts at: 0 1351 2031
Size: 1351 680
HaeII r_GCGC'y
Cuts at: 0 592 865 1640 2031
Size: 592 273 775 391
Fragments arranged by size:
775 592 391 273
HincII GTy'rAC
Cuts at: 0 1431 2031
Size: 1431 600
HindIII A'AGCT_T
Cuts at: 0 22 2031
Size: 22 2009
KasI G'GCGC_C
Cuts at: 0 588 2031
Size: 588 1443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1627 2031
Size: 1627 404
MslI CAynn'nnrTG
Cuts at: 0 58 175 2031
Size: 58 117 1856
Fragments arranged by size:
1856 117 58
MspA1I CmG'CkG
Cuts at: 0 111 919 1916 2031
Size: 111 808 997 115
Fragments arranged by size:
997 808 115 111
NarI GG'CG_CC
Cuts at: 0 589 2031
Size: 589 1442
NcoI C'CATG_G
Cuts at: 0 74 2031
Size: 74 1957
PflMI CCAn_nnn'nTGG
Cuts at: 0 250 1085 1495 1557 2031
Size: 250 835 410 62 474
Fragments arranged by size:
835 474 410 250 62
PfoI T'CCnGG_A
Cuts at: 0 1057 2031
Size: 1057 974
PpuMI rG'GwC_Cy
Cuts at: 0 1358 1656 2031
Size: 1358 298 375
Fragments arranged by size:
1358 375 298
PspOMI G'GGCC_C
Cuts at: 0 608 1366 1400 2031
Size: 608 758 34 631
Fragments arranged by size:
758 631 608 34
PstI C_TGCA'G
Cuts at: 0 1190 1418 2031
Size: 1190 228 613
Fragments arranged by size:
1190 613 228
PvuII CAG'CTG
Cuts at: 0 111 919 2031
Size: 111 808 1112
Fragments arranged by size:
1112 808 111
SanDI GG'GwC_CC
Cuts at: 0 1358 2031
Size: 1358 673
SfcI C'TryA_G
Cuts at: 0 1186 1414 2031
Size: 1186 228 617
Fragments arranged by size:
1186 617 228
SfoI GGC'GCC
Cuts at: 0 590 2031
Size: 590 1441
SmaI CCC'GGG
Cuts at: 0 370 2031
Size: 370 1661
StuI AGG'CCT
Cuts at: 0 688 2031
Size: 688 1343
StyI C'CwwG_G
Cuts at: 0 74 1362 2031
Size: 74 1288 669
Fragments arranged by size:
1288 669 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1077 1663 2031
Size: 1077 586 368
Fragments arranged by size:
1077 586 368
TatI w'GTAC_w
Cuts at: 0 1814 2031
Size: 1814 217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1197 1229 1532 1564 1854 1886 2031
Size: 1197 32 303 32 290 32 145
Fragments arranged by size:
1197 303 290 145 32 32 32
XbaI T'CTAG_A
Cuts at: 0 888 2031
Size: 888 1143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 1086 1134 1746 2031
Size: 60 1026 48 612 285
Fragments arranged by size:
1026 612 285 60 48
XmaI C'CCGG_G
Cuts at: 0 368 2031
Size: 368 1663
ZraI GAC'GTC
Cuts at: 0 384 2031
Size: 384 1647
Enzymes that do cut:
AatII AcuI AfeI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DrdI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII
HindIII KasI MmeI MslI MspA1I NarI NcoI PflMI
PfoI PpuMI PspOMI PstI PvuII SanDI SfcI SfoI
SmaI StuI StyI TaqII TatI TstI XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII EaeI EagI EarI EcoICRI EcoRV
FalI FseI FspI FspAI Hin4I HpaI KpnI MfeI
MluI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XhoI XmnI