Restriction map for
pF1KB6229
(Linear) (Six-base) MAPSORT of: KIBB6229.seq Check: 4210 from: 1 to: 2019
KIBB6229.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 669 729 903 1616 2019
Size: 669 60 174 713 403
Fragments arranged by size:
713 669 403 174 60
Acc65I G'GTAC_C
Cuts at: 0 452 2019
Size: 452 1567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 539 637 1086 2019
Size: 376 163 98 449 933
Fragments arranged by size:
933 449 376 163 98
AfeI AGC'GCT
Cuts at: 0 1492 1828 2019
Size: 1492 336 191
Fragments arranged by size:
1492 336 191
AleI CACnn'nnGTG
Cuts at: 0 1706 1790 2019
Size: 1706 84 229
Fragments arranged by size:
1706 229 84
AlwNI CAG_nnn'CTG
Cuts at: 0 249 354 2019
Size: 249 105 1665
Fragments arranged by size:
1665 249 105
ApaI G_GGCC'C
Cuts at: 0 536 671 2019
Size: 536 135 1348
Fragments arranged by size:
1348 536 135
ApaLI G'TGCA_C
Cuts at: 0 1400 1783 1893 2019
Size: 1400 383 110 126
Fragments arranged by size:
1400 383 126 110
AvaI C'yCGr_G
Cuts at: 0 670 1832 1960 2019
Size: 670 1162 128 59
Fragments arranged by size:
1162 670 128 59
BanI G'GyrC_C
Cuts at: 0 452 701 750 915 1369 1876 2019
Size: 452 249 49 165 454 507 143
Fragments arranged by size:
507 454 452 249 165 143 49
BanII G_rGCy'C
Cuts at: 0 536 553 671 1307 2019
Size: 536 17 118 636 712
Fragments arranged by size:
712 636 536 118 17
BbeI G_GCGC'C
Cuts at: 0 1880 2019
Size: 1880 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 730 1040 1294 1461 1999 2019
Size: 730 310 254 167 538 20
Fragments arranged by size:
730 538 310 254 167 20
BbvCI CC'TCA_GC
Cuts at: 0 716 929 2019
Size: 716 213 1090
Fragments arranged by size:
1090 716 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1391 1425 2019
Size: 1391 34 594
Fragments arranged by size:
1391 594 34
BclI T'GATC_A
Cuts at: 0 1389 1850 2019
Size: 1389 461 169
Fragments arranged by size:
1389 461 169
BfrBI ATG'CAT
Cuts at: 0 259 2019
Size: 259 1760
BglI GCCn_nnn'nGGC
Cuts at: 0 409 511 2019
Size: 409 102 1508
Fragments arranged by size:
1508 409 102
Bme1580I G_kGCm'C
Cuts at: 0 536 671 755 1404 1787 1897 2019
Size: 536 135 84 649 383 110 122
Fragments arranged by size:
649 536 383 135 122 110 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 346 361 385 1447 1786 2001 2019
Size: 271 75 15 24 1062 339 215 18
Fragments arranged by size:
1062 339 271 215 75 24 18 15
Bpu10I CC'TnA_GC
Cuts at: 0 716 929 959 2019
Size: 716 213 30 1060
Fragments arranged by size:
1060 716 213 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1666 1738 2019
Size: 442 1224 72 281
Fragments arranged by size:
1224 442 281 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 361 2019
Size: 361 1658
BsaAI yAC'GTr
Cuts at: 0 1071 2019
Size: 1071 948
BsaHI Gr'CG_yC
Cuts at: 0 1877 2019
Size: 1877 142
BsaWI w'CCGG_w
Cuts at: 0 143 2019
Size: 143 1876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 334 349 364 484 509 514 539
Size: 319 15 15 15 120 25 5 25
Cuts at: 539 620 650 1813 1843 2019
Size: 81 30 1163 30 176
Fragments arranged by size:
1163 319 176 120 81 30 30 25
25 15 15 15 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 688 913 1917 1978 2019
Size: 505 183 225 1004 61 41
Fragments arranged by size:
1004 505 225 183 61 41
BseYI C'CCAG_C
Cuts at: 0 688 754 924 966 1161 1582 1918 2019
Size: 688 66 170 42 195 421 336 101
Fragments arranged by size:
688 421 336 195 170 101 66 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1093 1236 1258 1887 2019
Size: 144 949 143 22 629 132
Fragments arranged by size:
949 629 144 143 132 22
BsiEI CG_ry'CG
Cuts at: 0 317 2019
Size: 317 1702
BsiHKAI G_wGCw'C
Cuts at: 0 965 1307 1404 1678 1787 1897 2019
Size: 965 342 97 274 109 110 122
Fragments arranged by size:
965 342 274 122 110 109 97
BsiWI C'GTAC_G
Cuts at: 0 1292 2019
Size: 1292 727
BsmI GAATG_Cn'
Cuts at: 0 480 1349 2019
Size: 480 869 670
Fragments arranged by size:
869 670 480
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1762 2019
Size: 1762 257
Bsp1286I G_dGCh'C
Cuts at: 0 536 553 671 755 965 1307 1404 1678
Size: 536 17 118 84 210 342 97 274
Cuts at: 1678 1787 1897 2019
Size: 109 110 122
Fragments arranged by size:
536 342 274 210 122 118 110 109
97 84 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 669 729 903 1616 2019
Size: 311 358 60 174 713 403
Fragments arranged by size:
713 403 358 311 174 60
BsrDI GCAATG_nn'
Cuts at: 0 1734 2019
Size: 1734 285
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1565 1901 2019
Size: 1565 336 118
Fragments arranged by size:
1565 336 118
BstBI TT'CG_AA
Cuts at: 0 1411 2019
Size: 1411 608
BstYI r'GATC_y
Cuts at: 0 3 179 320 1967 2019
Size: 3 176 141 1647 52
Fragments arranged by size:
1647 176 141 52 3
BtgI C'CryG_G
Cuts at: 0 50 407 536 1094 2019
Size: 50 357 129 558 925
Fragments arranged by size:
925 558 357 129 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 2019
Size: 116 1903
BtsI GCAGTG_nn'
Cuts at: 0 611 1219 1510 1566 2019
Size: 611 608 291 56 453
Fragments arranged by size:
611 608 453 291 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 552 587 1716 1751 2019
Size: 552 35 1129 35 268
Fragments arranged by size:
1129 552 268 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1465 2019
Size: 1465 554
EaeI y'GGCC_r
Cuts at: 0 86 314 410 936 951 1463 1844 2019
Size: 86 228 96 526 15 512 381 175
Fragments arranged by size:
526 512 381 228 175 96 86 15
EagI C'GGCC_G
Cuts at: 0 314 2019
Size: 314 1705
EarI CTCTTCn'nnn_
Cuts at: 0 190 428 656 2019
Size: 190 238 228 1363
Fragments arranged by size:
1363 238 228 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 2019
Size: 301 1718
EcoICRI GAG'CTC
Cuts at: 0 1305 2019
Size: 1305 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 346 361 376 385 539 637 1086
Size: 271 75 15 15 9 154 98 449
Cuts at: 1086 1447 1786 2001 2019
Size: 361 339 215 18
Fragments arranged by size:
449 361 339 271 215 154 98 75
18 15 15 9
EcoO109I rG'GnC_Cy
Cuts at: 0 291 667 835 842 942 2019
Size: 291 376 168 7 100 1077
Fragments arranged by size:
1077 376 291 168 100 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 2019
Size: 483 1536
FspI TGC'GCA
Cuts at: 0 1546 2019
Size: 1546 473
HaeII r_GCGC'y
Cuts at: 0 1494 1830 1880 2019
Size: 1494 336 50 139
Fragments arranged by size:
1494 336 139 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 509 541 620 652 834 866
Size: 208 32 269 32 79 32 182 32
Cuts at: 866 2019
Size: 1153
Fragments arranged by size:
1153 269 208 182 79 32 32 32
32
HincII GTy'rAC
Cuts at: 0 202 2019
Size: 202 1817
KasI G'GCGC_C
Cuts at: 0 1876 2019
Size: 1876 143
KpnI G_GTAC'C
Cuts at: 0 456 2019
Size: 456 1563
MscI TGG'CCA
Cuts at: 0 938 2019
Size: 938 1081
MslI CAynn'nnrTG
Cuts at: 0 115 1459 1706 1790 2019
Size: 115 1344 247 84 229
Fragments arranged by size:
1344 247 229 115 84
MspA1I CmG'CkG
Cuts at: 0 30 928 1366 1658 1764 2019
Size: 30 898 438 292 106 255
Fragments arranged by size:
898 438 292 255 106 30
NarI GG'CG_CC
Cuts at: 0 1877 2019
Size: 1877 142
NcoI C'CATG_G
Cuts at: 0 407 536 1094 2019
Size: 407 129 558 925
Fragments arranged by size:
925 558 407 129
NsiI A_TGCA'T
Cuts at: 0 261 2019
Size: 261 1758
NspI r_CATG'y
Cuts at: 0 1044 2019
Size: 1044 975
PasI CC'CwG_GG
Cuts at: 0 529 706 769 1128 2019
Size: 529 177 63 359 891
Fragments arranged by size:
891 529 359 177 63
PfoI T'CCnGG_A
Cuts at: 0 323 1322 1664 2019
Size: 323 999 342 355
Fragments arranged by size:
999 355 342 323
PmlI CAC'GTG
Cuts at: 0 1071 2019
Size: 1071 948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1630 1662 2019
Size: 1630 32 357
Fragments arranged by size:
1630 357 32
PpuMI rG'GwC_Cy
Cuts at: 0 291 842 2019
Size: 291 551 1177
Fragments arranged by size:
1177 551 291
PspOMI G'GGCC_C
Cuts at: 0 532 667 2019
Size: 532 135 1352
Fragments arranged by size:
1352 532 135
PstI C_TGCA'G
Cuts at: 0 35 606 644 666 1517 1561 2019
Size: 35 571 38 22 851 44 458
Fragments arranged by size:
851 571 458 44 38 35 22
PvuII CAG'CTG
Cuts at: 0 30 928 1658 1764 2019
Size: 30 898 730 106 255
Fragments arranged by size:
898 730 255 106 30
SacI G_AGCT'C
Cuts at: 0 1307 2019
Size: 1307 712
SanDI GG'GwC_CC
Cuts at: 0 842 2019
Size: 842 1177
SbfI CC_TGCA'GG
Cuts at: 0 644 666 2019
Size: 644 22 1353
Fragments arranged by size:
1353 644 22
ScaI AGT'ACT
Cuts at: 0 1912 2019
Size: 1912 107
SfcI C'TryA_G
Cuts at: 0 31 602 640 662 1513 1557 1776 2019
Size: 31 571 38 22 851 44 219 243
Fragments arranged by size:
851 571 243 219 44 38 31 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 409 2019
Size: 409 1610
SfoI GGC'GCC
Cuts at: 0 1878 2019
Size: 1878 141
SmaI CCC'GGG
Cuts at: 0 1834 2019
Size: 1834 185
SmlI C'TyrA_G
Cuts at: 0 457 1681 1753 2019
Size: 457 1224 72 266
Fragments arranged by size:
1224 457 266 72
SphI G_CATG'C
Cuts at: 0 1044 2019
Size: 1044 975
StuI AGG'CCT
Cuts at: 0 379 2019
Size: 379 1640
StyI C'CwwG_G
Cuts at: 0 281 407 536 596 1094 2019
Size: 281 126 129 60 498 925
Fragments arranged by size:
925 498 281 129 126 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 980 2019
Size: 980 1039
TatI w'GTAC_w
Cuts at: 0 1910 2019
Size: 1910 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 334 366 1813 1845 2019
Size: 334 32 1447 32 174
Fragments arranged by size:
1447 334 174 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 766 946 2019
Size: 603 163 180 1073
Fragments arranged by size:
1073 603 180 163
XmaI C'CCGG_G
Cuts at: 0 1832 2019
Size: 1832 187
XmnI GAAnn'nnTTC
Cuts at: 0 466 1318 1492 2019
Size: 466 852 174 527
Fragments arranged by size:
852 527 466 174
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII
Hin4I HincII KasI KpnI MscI MslI MspA1I NarI
NcoI NsiI NspI PasI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SbfI ScaI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BglII BlpI BmgBI BmrI BmtI BplI BsaBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I ClaI DraI DraIII EcoNI EcoRI
EcoRV FseI FspAI HindIII HpaI MfeI MluI MmeI
NaeI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SexAI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI ZraI