Restriction map for
pF1KB6217
(Linear) (Six-base) MAPSORT of: KIBB6217.seq Check: 6113 from: 1 to: 2184
KIBB6217.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1407 2184
Size: 1407 777
Acc65I G'GTAC_C
Cuts at: 0 593 929 2184
Size: 593 336 1255
Fragments arranged by size:
1255 593 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 2184
Size: 489 1695
AleI CACnn'nnGTG
Cuts at: 0 685 2184
Size: 685 1499
AlwNI CAG_nnn'CTG
Cuts at: 0 267 747 1216 1532 2068 2184
Size: 267 480 469 316 536 116
Fragments arranged by size:
536 480 469 316 267 116
ApaI G_GGCC'C
Cuts at: 0 64 274 777 1224 2122 2183 2184
Size: 64 210 503 447 898 61 1
Fragments arranged by size:
898 503 447 210 64 61 1
ApaLI G'TGCA_C
Cuts at: 0 1234 2184
Size: 1234 950
AvaI C'yCGr_G
Cuts at: 0 26 1223 2184
Size: 26 1197 961
Fragments arranged by size:
1197 961 26
BanI G'GyrC_C
Cuts at: 0 285 385 593 929 2184
Size: 285 100 208 336 1255
Fragments arranged by size:
1255 336 285 208 100
BanII G_rGCy'C
Cuts at: 0 64 71 116 236 274 350 777 786
Size: 64 7 45 120 38 76 427 9
Cuts at: 786 1224 1892 2122 2143 2183 2184
Size: 438 668 230 21 40 1
Fragments arranged by size:
668 438 427 230 120 76 64 45
40 38 21 9 7 1
BbeI G_GCGC'C
Cuts at: 0 289 389 2184
Size: 289 100 1795
Fragments arranged by size:
1795 289 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 430 546 1124 1674 2184
Size: 430 116 578 550 510
Fragments arranged by size:
578 550 510 430 116
BclI T'GATC_A
Cuts at: 0 1451 2184
Size: 1451 733
BglI GCCn_nnn'nGGC
Cuts at: 0 436 1970 2184
Size: 436 1534 214
Fragments arranged by size:
1534 436 214
BlpI GC'TnA_GC
Cuts at: 0 70 1886 2184
Size: 70 1816 298
Fragments arranged by size:
1816 298 70
Bme1580I G_kGCm'C
Cuts at: 0 55 64 274 777 1224 1238 2122 2183
Size: 55 9 210 503 447 14 884 61
Cuts at: 2183 2184
Size: 1
Fragments arranged by size:
884 503 447 210 61 55 14 9
1
BmrI ACTGGGnnnn_n'
Cuts at: 0 1226 2184
Size: 1226 958
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 222 1812 2010 2184
Size: 222 1590 198 174
Fragments arranged by size:
1590 222 198 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 212 214 637 783 1006 1540 1943
Size: 154 58 2 423 146 223 534 403
Cuts at: 1943 2063 2117 2184
Size: 120 54 67
Fragments arranged by size:
534 423 403 223 154 146 120 67
58 54 2
Bpu10I CC'TnA_GC
Cuts at: 0 1982 2184
Size: 1982 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 1041 1291 1961 2184
Size: 178 863 250 670 223
Fragments arranged by size:
863 670 250 223 178
BsaHI Gr'CG_yC
Cuts at: 0 286 386 2184
Size: 286 100 1798
Fragments arranged by size:
1798 286 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1021 1051 2079 2109 2184
Size: 1021 30 1028 30 75
Fragments arranged by size:
1028 1021 75 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 211 271 322 325 625 634 1003
Size: 151 60 60 51 3 300 9 369
Cuts at: 1003 1560 1848 2184
Size: 557 288 336
Fragments arranged by size:
557 369 336 300 288 151 60 60
51 9 3
BseYI C'CCAG_C
Cuts at: 0 267 290 407 999 2057 2184
Size: 267 23 117 592 1058 127
Fragments arranged by size:
1058 592 267 127 117 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 558 1341 2184
Size: 438 120 783 843
Fragments arranged by size:
843 783 438 120
BsiHKAI G_wGCw'C
Cuts at: 0 71 350 406 1238 1892 1983 2184
Size: 71 279 56 832 654 91 201
Fragments arranged by size:
832 654 279 201 91 71 56
Bsp1286I G_dGCh'C
Cuts at: 0 55 64 71 116 236 274 350 406
Size: 55 9 7 45 120 38 76 56
Cuts at: 406 777 786 1224 1238 1892 1983 2122 2143
Size: 371 9 438 14 654 91 139 21
Cuts at: 2143 2183 2184
Size: 40 1
Fragments arranged by size:
654 438 371 139 120 91 76 56
55 45 40 38 21 14 9 9
7 1
BspHI T'CATG_A
Cuts at: 0 116 236 2184
Size: 116 120 1948
Fragments arranged by size:
1948 120 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 722 1260 1407 1910 2184
Size: 288 434 538 147 503 274
Fragments arranged by size:
538 503 434 288 274 147
BsrBI CCG'CTC
Cuts at: 0 335 2184
Size: 335 1849
BsrFI r'CCGG_y
Cuts at: 0 691 2184
Size: 691 1493
BsrGI T'GTAC_A
Cuts at: 0 659 2184
Size: 659 1525
BssHII G'CGCG_C
Cuts at: 0 1173 2184
Size: 1173 1011
BssSI C'ACGA_G
Cuts at: 0 685 2184
Size: 685 1499
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 2184
Size: 738 1446
BstEII G'GTnAC_C
Cuts at: 0 1809 2184
Size: 1809 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 687 2184
Size: 396 291 1497
Fragments arranged by size:
1497 396 291
Bsu36I CC'TnA_GG
Cuts at: 0 748 2184
Size: 748 1436
BtgI C'CryG_G
Cuts at: 0 299 381 1486 2184
Size: 299 82 1105 698
Fragments arranged by size:
1105 698 299 82
BtsI GCAGTG_nn'
Cuts at: 0 446 569 1377 2184
Size: 446 123 808 807
Fragments arranged by size:
808 807 446 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1443 1478 2184
Size: 1443 35 706
Fragments arranged by size:
1443 706 35
DraIII CAC_nnn'GTG
Cuts at: 0 1598 2184
Size: 1598 586
DrdI GACnn_nn'nnGTC
Cuts at: 0 550 1906 2184
Size: 550 1356 278
Fragments arranged by size:
1356 550 278
EaeI y'GGCC_r
Cuts at: 0 8 689 1739 2184
Size: 8 681 1050 445
Fragments arranged by size:
1050 681 445 8
EarI CTCTTCn'nnn_
Cuts at: 0 355 2184
Size: 355 1829
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 319 382 2151 2184
Size: 319 63 1769 33
Fragments arranged by size:
1769 319 63 33
EcoICRI GAG'CTC
Cuts at: 0 69 348 1890 2184
Size: 69 279 1542 294
Fragments arranged by size:
1542 294 279 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 212 214 489 637 783 1006 1540
Size: 154 58 2 275 148 146 223 534
Cuts at: 1540 1943 2063 2117 2184
Size: 403 120 54 67
Fragments arranged by size:
534 403 275 223 154 148 146 120
67 58 54 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 2184
Size: 988 1196
EcoO109I rG'GnC_Cy
Cuts at: 0 60 144 271 315 753 773 1220 2065
Size: 60 84 127 44 438 20 447 845
Cuts at: 2065 2119 2179 2184
Size: 54 60 5
Fragments arranged by size:
845 447 438 127 84 60 60 54
44 20 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 570 867 2184
Size: 447 123 297 1317
Fragments arranged by size:
1317 447 297 123
HaeII r_GCGC'y
Cuts at: 0 289 389 747 2184
Size: 289 100 358 1437
Fragments arranged by size:
1437 358 289 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1555 1587 2079 2111 2184
Size: 76 32 1447 32 492 32 73
Fragments arranged by size:
1447 492 76 73 32 32 32
HincII GTy'rAC
Cuts at: 0 1563 1719 1749 2184
Size: 1563 156 30 435
Fragments arranged by size:
1563 435 156 30
KasI G'GCGC_C
Cuts at: 0 285 385 2184
Size: 285 100 1799
Fragments arranged by size:
1799 285 100
KpnI G_GTAC'C
Cuts at: 0 597 933 2184
Size: 597 336 1251
Fragments arranged by size:
1251 597 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1847 2184
Size: 1142 705 337
Fragments arranged by size:
1142 705 337
MscI TGG'CCA
Cuts at: 0 10 1741 2184
Size: 10 1731 443
Fragments arranged by size:
1731 443 10
MslI CAynn'nnrTG
Cuts at: 0 685 1309 2184
Size: 685 624 875
Fragments arranged by size:
875 685 624
MspA1I CmG'CkG
Cuts at: 0 858 1488 2184
Size: 858 630 696
Fragments arranged by size:
858 696 630
NaeI GCC'GGC
Cuts at: 0 693 2184
Size: 693 1491
NarI GG'CG_CC
Cuts at: 0 286 386 2184
Size: 286 100 1798
Fragments arranged by size:
1798 286 100
NgoMIV G'CCGG_C
Cuts at: 0 691 2184
Size: 691 1493
NruI TCG'CGA
Cuts at: 0 710 1698 2184
Size: 710 988 486
Fragments arranged by size:
988 710 486
PasI CC'CwG_GG
Cuts at: 0 2115 2184
Size: 2115 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 1124 1500 2184
Size: 1124 376 684
Fragments arranged by size:
1124 684 376
PfoI T'CCnGG_A
Cuts at: 0 1439 2184
Size: 1439 745
PpuMI rG'GwC_Cy
Cuts at: 0 144 753 2184
Size: 144 609 1431
Fragments arranged by size:
1431 609 144
PspOMI G'GGCC_C
Cuts at: 0 60 270 773 1220 2118 2179 2184
Size: 60 210 503 447 898 61 5
Fragments arranged by size:
898 503 447 210 61 60 5
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 2184
Size: 1489 32 663
Fragments arranged by size:
1489 663 32
PstI C_TGCA'G
Cuts at: 0 161 2148 2184
Size: 161 1987 36
Fragments arranged by size:
1987 161 36
PvuII CAG'CTG
Cuts at: 0 858 2184
Size: 858 1326
SacI G_AGCT'C
Cuts at: 0 71 350 1892 2184
Size: 71 279 1542 292
Fragments arranged by size:
1542 292 279 71
SacII CC_GC'GG
Cuts at: 0 1489 2184
Size: 1489 695
SanDI GG'GwC_CC
Cuts at: 0 753 2184
Size: 753 1431
SapI GCTCTTCn'nnn_
Cuts at: 0 355 2184
Size: 355 1829
ScaI AGT'ACT
Cuts at: 0 502 1507 2184
Size: 502 1005 677
Fragments arranged by size:
1005 677 502
SexAI A'CCwGG_T
Cuts at: 0 168 1031 2184
Size: 168 863 1153
Fragments arranged by size:
1153 863 168
SfcI C'TryA_G
Cuts at: 0 157 588 1515 2144 2184
Size: 157 431 927 629 40
Fragments arranged by size:
927 629 431 157 40
SfoI GGC'GCC
Cuts at: 0 287 387 2184
Size: 287 100 1797
Fragments arranged by size:
1797 287 100
SmaI CCC'GGG
Cuts at: 0 1225 2184
Size: 1225 959
TatI w'GTAC_w
Cuts at: 0 470 500 659 1406 1505 2184
Size: 470 30 159 747 99 679
Fragments arranged by size:
747 679 470 159 99 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 237 269 567 599 2184
Size: 15 32 190 32 298 32 1585
Fragments arranged by size:
1585 298 190 32 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1078 1636 2184
Size: 1078 558 548
Fragments arranged by size:
1078 558 548
XmaI C'CCGG_G
Cuts at: 0 1223 2184
Size: 1223 961
XmnI GAAnn'nnTTC
Cuts at: 0 1189 2184
Size: 1189 995
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI PasI PflMI PfoI PpuMI PspOMI PsrI PstI
PvuII SacI SacII SanDI SapI ScaI SexAI SfcI
SfoI SmaI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrDI BstBI BstYI BstZ17I BtgZI ClaI DraI
EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
MfeI MluI NcoI NdeI NheI NotI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SalI SbfI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII Tth111I XbaI XhoI ZraI