Restriction map for
pF1KB6215
(Linear) (Six-base) MAPSORT of: KIBB6215.seq Check: 7932 from: 1 to: 2007
KIBB6215.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 470 910 1496 2007
Size: 470 440 586 511
Fragments arranged by size:
586 511 470 440
AfeI AGC'GCT
Cuts at: 0 262 391 1987 2007
Size: 262 129 1596 20
Fragments arranged by size:
1596 262 129 20
AflIII A'CryG_T
Cuts at: 0 1965 2007
Size: 1965 42
AgeI A'CCGG_T
Cuts at: 0 283 643 2007
Size: 283 360 1364
Fragments arranged by size:
1364 360 283
AleI CACnn'nnGTG
Cuts at: 0 286 1742 2007
Size: 286 1456 265
Fragments arranged by size:
1456 286 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 757 2007
Size: 757 1250
AlwNI CAG_nnn'CTG
Cuts at: 0 1359 1457 1517 2007
Size: 1359 98 60 490
Fragments arranged by size:
1359 490 98 60
ApaI G_GGCC'C
Cuts at: 0 1054 2007
Size: 1054 953
ApoI r'AATT_y
Cuts at: 0 1606 2007
Size: 1606 401
AvaI C'yCGr_G
Cuts at: 0 12 874 1067 1753 2007
Size: 12 862 193 686 254
Fragments arranged by size:
862 686 254 193 12
AvrII C'CTAG_G
Cuts at: 0 684 2007
Size: 684 1323
BamHI G'GATC_C
Cuts at: 0 272 1526 2007
Size: 272 1254 481
Fragments arranged by size:
1254 481 272
BanI G'GyrC_C
Cuts at: 0 336 470 543 640 831 910 1496 1591
Size: 336 134 73 97 191 79 586 95
Cuts at: 1591 1890 2007
Size: 299 117
Fragments arranged by size:
586 336 299 191 134 117 97 95
79 73
BanII G_rGCy'C
Cuts at: 0 719 1054 1343 1421 1637 1754 2007
Size: 719 335 289 78 216 117 253
Fragments arranged by size:
719 335 289 253 216 117 78
BbeI G_GCGC'C
Cuts at: 0 835 2007
Size: 835 1172
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 525 2007
Size: 146 379 1482
Fragments arranged by size:
1482 379 146
BbvCI CC'TCA_GC
Cuts at: 0 191 2007
Size: 191 1816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 67 2007
Size: 33 34 1940
Fragments arranged by size:
1940 34 33
BglI GCCn_nnn'nGGC
Cuts at: 0 27 53 829 966 975 996 1035 1224
Size: 27 26 776 137 9 21 39 189
Cuts at: 1224 2007
Size: 783
Fragments arranged by size:
783 776 189 137 39 27 26 21
9
BglII A'GATC_T
Cuts at: 0 230 2007
Size: 230 1777
Bme1580I G_kGCm'C
Cuts at: 0 548 1054 1229 1594 2007
Size: 548 506 175 365 413
Fragments arranged by size:
548 506 413 365 175
BmgBI CAC'GTC
Cuts at: 0 1713 1779 2007
Size: 1713 66 228
Fragments arranged by size:
1713 228 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 271 1051 2007
Size: 271 780 956
Fragments arranged by size:
956 780 271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 792 820 1357 2007
Size: 714 78 28 537 650
Fragments arranged by size:
714 650 537 78 28
Bpu10I CC'TnA_GC
Cuts at: 0 191 1555 2007
Size: 191 1364 452
Fragments arranged by size:
1364 452 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 1481 2007
Size: 295 1186 526
Fragments arranged by size:
1186 526 295
BsaAI yAC'GTr
Cuts at: 0 1686 1966 2007
Size: 1686 280 41
Fragments arranged by size:
1686 280 41
BsaHI Gr'CG_yC
Cuts at: 0 381 832 958 2007
Size: 381 451 126 1049
Fragments arranged by size:
1049 451 381 126
BsaWI w'CCGG_w
Cuts at: 0 283 643 2007
Size: 283 360 1364
Fragments arranged by size:
1364 360 283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 79 1486 1867 2007
Size: 40 39 1407 381 140
Fragments arranged by size:
1407 381 140 40 39
BseYI C'CCAG_C
Cuts at: 0 196 780 792 938 1004 2007
Size: 196 584 12 146 66 1003
Fragments arranged by size:
1003 584 196 146 66 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1766 1864 1960 2007
Size: 1766 98 96 47
Fragments arranged by size:
1766 98 96 47
BsiEI CG_ry'CG
Cuts at: 0 31 60 79 137 991 1284 1723 2007
Size: 31 29 19 58 854 293 439 284
Fragments arranged by size:
854 439 293 284 58 31 29 19
BsiHKAI G_wGCw'C
Cuts at: 0 293 806 1685 2007
Size: 293 513 879 322
Fragments arranged by size:
879 513 322 293
Bsp1286I G_dGCh'C
Cuts at: 0 293 548 719 806 1054 1229 1343 1421
Size: 293 255 171 87 248 175 114 78
Cuts at: 1421 1594 1637 1685 1754 2007
Size: 173 43 48 69 253
Fragments arranged by size:
293 255 253 248 175 173 171 114
87 78 69 48 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 380 749 2007
Size: 194 186 369 1258
Fragments arranged by size:
1258 369 194 186
BsrBI CCG'CTC
Cuts at: 0 1035 1080 2007
Size: 1035 45 927
Fragments arranged by size:
1035 927 45
BsrDI GCAATG_nn'
Cuts at: 0 1566 2007
Size: 1566 441
BsrFI r'CCGG_y
Cuts at: 0 283 643 974 986 2007
Size: 283 360 331 12 1021
Fragments arranged by size:
1021 360 331 283 12
BssHII G'CGCG_C
Cuts at: 0 17 2007
Size: 17 1990
BstEII G'GTnAC_C
Cuts at: 0 1303 2007
Size: 1303 704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 2007
Size: 528 1479
BstYI r'GATC_y
Cuts at: 0 230 272 1526 2007
Size: 230 42 1254 481
Fragments arranged by size:
1254 481 230 42
BtgI C'CryG_G
Cuts at: 0 615 888 961 1737 2007
Size: 615 273 73 776 270
Fragments arranged by size:
776 615 273 270 73
BtsI GCAGTG_nn'
Cuts at: 0 776 1894 2007
Size: 776 1118 113
Fragments arranged by size:
1118 776 113
DraIII CAC_nnn'GTG
Cuts at: 0 1483 2007
Size: 1483 524
DrdI GACnn_nn'nnGTC
Cuts at: 0 148 2007
Size: 148 1859
EaeI y'GGCC_r
Cuts at: 0 28 76 134 967 988 1130 1403 1720
Size: 28 48 58 833 21 142 273 317
Cuts at: 1720 2007
Size: 287
Fragments arranged by size:
833 317 287 273 142 58 48 28
21
EagI C'GGCC_G
Cuts at: 0 28 76 134 988 1720 2007
Size: 28 48 58 854 732 287
Fragments arranged by size:
854 732 287 58 48 28
EarI CTCTTCn'nnn_
Cuts at: 0 293 763 2007
Size: 293 470 1244
Fragments arranged by size:
1244 470 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 213 1774 2007
Size: 213 1561 233
Fragments arranged by size:
1561 233 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 792 820 1357 2007
Size: 714 78 28 537 650
Fragments arranged by size:
714 650 537 78 28
EcoO109I rG'GnC_Cy
Cuts at: 0 497 1051 1547 1696 2007
Size: 497 554 496 149 311
Fragments arranged by size:
554 497 496 311 149
FseI GG_CCGG'CC
Cuts at: 0 978 2007
Size: 978 1029
FspI TGC'GCA
Cuts at: 0 493 2007
Size: 493 1514
HaeII r_GCGC'y
Cuts at: 0 264 393 835 1275 1464 1623 1989 2007
Size: 264 129 442 440 189 159 366 18
Fragments arranged by size:
442 440 366 264 189 159 129 18
HincII GTy'rAC
Cuts at: 0 858 1815 2007
Size: 858 957 192
Fragments arranged by size:
957 858 192
KasI G'GCGC_C
Cuts at: 0 831 2007
Size: 831 1176
KpnI G_GTAC'C
Cuts at: 0 474 914 1500 2007
Size: 474 440 586 507
Fragments arranged by size:
586 507 474 440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 2007
Size: 364 1643
MscI TGG'CCA
Cuts at: 0 1132 2007
Size: 1132 875
MslI CAynn'nnrTG
Cuts at: 0 286 1742 2007
Size: 286 1456 265
Fragments arranged by size:
1456 286 265
MspA1I CmG'CkG
Cuts at: 0 93 329 1169 1359 2007
Size: 93 236 840 190 648
Fragments arranged by size:
840 648 236 190 93
NaeI GCC'GGC
Cuts at: 0 976 2007
Size: 976 1031
NarI GG'CG_CC
Cuts at: 0 832 2007
Size: 832 1175
NdeI CA'TA_TG
Cuts at: 0 206 2007
Size: 206 1801
NgoMIV G'CCGG_C
Cuts at: 0 974 2007
Size: 974 1033
NotI GC'GGCC_GC
Cuts at: 0 76 2007
Size: 76 1931
PasI CC'CwG_GG
Cuts at: 0 1492 2007
Size: 1492 515
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 311 480 792 1412 1601 2007
Size: 234 77 169 312 620 189 406
Fragments arranged by size:
620 406 312 234 189 169 77
PfoI T'CCnGG_A
Cuts at: 0 536 1199 2007
Size: 536 663 808
Fragments arranged by size:
808 663 536
PmlI CAC'GTG
Cuts at: 0 1686 1966 2007
Size: 1686 280 41
Fragments arranged by size:
1686 280 41
PspOMI G'GGCC_C
Cuts at: 0 1050 2007
Size: 1050 957
PstI C_TGCA'G
Cuts at: 0 1997 2007
Size: 1997 10
SfcI C'TryA_G
Cuts at: 0 1993 2007
Size: 1993 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 975 996 2007
Size: 975 21 1011
Fragments arranged by size:
1011 975 21
SfoI GGC'GCC
Cuts at: 0 833 2007
Size: 833 1174
SgrAI Cr'CCGG_yG
Cuts at: 0 283 643 2007
Size: 283 360 1364
Fragments arranged by size:
1364 360 283
SmaI CCC'GGG
Cuts at: 0 1755 2007
Size: 1755 252
StyI C'CwwG_G
Cuts at: 0 123 305 684 2007
Size: 123 182 379 1323
Fragments arranged by size:
1323 379 182 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 2007
Size: 712 1295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 825 1371 1700 2007
Size: 825 546 329 307
Fragments arranged by size:
825 546 329 307
XmaI C'CCGG_G
Cuts at: 0 1753 2007
Size: 1753 254
Enzymes that do cut:
Acc65I AfeI AflIII AgeI AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FseI FspI HaeII
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NotI PasI PflMI PfoI PmlI
PspOMI PstI SfcI SfiI SfoI SgrAI SmaI StyI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AhdI AloI
ApaLI AscI AseI AsiSI BaeI BciVI BclI BfrBI
BlpI BmtI BplI BpuEI BsaBI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRI
EcoRV FalI FspAI Hin4I HindIII HpaI MfeI MluI
NcoI NheI NruI NsiI NspI PacI PciI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI