Restriction map for
pF1KB6181
(Linear) (Six-base) MAPSORT of: KIBB6181.seq Check: 6384 from: 1 to: 1977
KIBB6181.orf
With 182 enzymes: SgfI *
July 28, 2009 13:23 ..
AatII G_ACGT'C
Cuts at: 0 1488 1977
Size: 1488 489
AccI GT'mk_AC
Cuts at: 0 1808 1977
Size: 1808 169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1645 1977
Size: 1213 432 332
Fragments arranged by size:
1213 432 332
AlwNI CAG_nnn'CTG
Cuts at: 0 210 1977
Size: 210 1767
ApaI G_GGCC'C
Cuts at: 0 688 1977
Size: 688 1289
ApoI r'AATT_y
Cuts at: 0 583 1607 1936 1977
Size: 583 1024 329 41
Fragments arranged by size:
1024 583 329 41
AseI AT'TA_AT
Cuts at: 0 1180 1977
Size: 1180 797
BanI G'GyrC_C
Cuts at: 0 49 86 1977
Size: 49 37 1891
Fragments arranged by size:
1891 49 37
BanII G_rGCy'C
Cuts at: 0 681 688 1977
Size: 681 7 1289
Fragments arranged by size:
1289 681 7
BbeI G_GCGC'C
Cuts at: 0 90 1977
Size: 90 1887
BbsI GAAGACnn'nnnn_
Cuts at: 0 750 876 1977
Size: 750 126 1101
Fragments arranged by size:
1101 750 126
BclI T'GATC_A
Cuts at: 0 1326 1977
Size: 1326 651
Bme1580I G_kGCm'C
Cuts at: 0 688 1977
Size: 688 1289
BmgBI CAC'GTC
Cuts at: 0 1812 1977
Size: 1812 165
BmrI ACTGGGnnnn_n'
Cuts at: 0 1761 1977
Size: 1761 216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 487 1977
Size: 487 1490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1977
Size: 1310 667
Bpu10I CC'TnA_GC
Cuts at: 0 680 1977
Size: 680 1297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1021 1088 1977
Size: 177 844 67 889
Fragments arranged by size:
889 844 177 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 1236 1364 1524 1977
Size: 1236 128 160 453
Fragments arranged by size:
1236 453 160 128
BsaBI GATnn'nnATC
Cuts at: 0 1202 1977
Size: 1202 775
BsaHI Gr'CG_yC
Cuts at: 0 87 1485 1977
Size: 87 1398 492
Fragments arranged by size:
1398 492 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1250 1280 1977
Size: 1250 30 697
Fragments arranged by size:
1250 697 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 505 1977
Size: 27 478 1472
Fragments arranged by size:
1472 478 27
BseYI C'CCAG_C
Cuts at: 0 687 1129 1679 1977
Size: 687 442 550 298
Fragments arranged by size:
687 550 442 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1817 1977
Size: 88 1729 160
Fragments arranged by size:
1729 160 88
BsiHKAI G_wGCw'C
Cuts at: 0 681 1977
Size: 681 1296
BsmI GAATG_Cn'
Cuts at: 0 1091 1792 1977
Size: 1091 701 185
Fragments arranged by size:
1091 701 185
Bsp1286I G_dGCh'C
Cuts at: 0 681 688 1977
Size: 681 7 1289
Fragments arranged by size:
1289 681 7
BspHI T'CATG_A
Cuts at: 0 1896 1977
Size: 1896 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1916 1977
Size: 1916 61
BsrFI r'CCGG_y
Cuts at: 0 1153 1977
Size: 1153 824
BstBI TT'CG_AA
Cuts at: 0 741 1977
Size: 741 1236
BstEII G'GTnAC_C
Cuts at: 0 985 1977
Size: 985 992
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 372 1753 1977
Size: 372 1381 224
Fragments arranged by size:
1381 372 224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 963 998 1977
Size: 963 35 979
Fragments arranged by size:
979 963 35
DraI TTT'AAA
Cuts at: 0 1202 1977
Size: 1202 775
EaeI y'GGCC_r
Cuts at: 0 1151 1977
Size: 1151 826
EarI CTCTTCn'nnn_
Cuts at: 0 1864 1977
Size: 1864 113
EcoICRI GAG'CTC
Cuts at: 0 679 1977
Size: 679 1298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1310 1645 1977
Size: 1213 97 335 332
Fragments arranged by size:
1213 335 332 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 1502 1977
Size: 487 1015 475
Fragments arranged by size:
1015 487 475
EcoO109I rG'GnC_Cy
Cuts at: 0 684 1977
Size: 684 1293
EcoRI G'AATT_C
Cuts at: 0 1936 1977
Size: 1936 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 1650 1977
Size: 390 1260 327
Fragments arranged by size:
1260 390 327
HaeII r_GCGC'y
Cuts at: 0 90 1977
Size: 90 1887
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 362 394 1250 1282 1977
Size: 362 32 856 32 695
Fragments arranged by size:
856 695 362 32 32
HincII GTy'rAC
Cuts at: 0 662 1809 1977
Size: 662 1147 168
Fragments arranged by size:
1147 662 168
HindIII A'AGCT_T
Cuts at: 0 503 1977
Size: 503 1474
HpaI GTT'AAC
Cuts at: 0 662 1977
Size: 662 1315
KasI G'GCGC_C
Cuts at: 0 86 1977
Size: 86 1891
MfeI C'AATT_G
Cuts at: 0 671 1977
Size: 671 1306
MslI CAynn'nnrTG
Cuts at: 0 1916 1977
Size: 1916 61
MspA1I CmG'CkG
Cuts at: 0 561 1977
Size: 561 1416
NarI GG'CG_CC
Cuts at: 0 87 1977
Size: 87 1890
NdeI CA'TA_TG
Cuts at: 0 1913 1977
Size: 1913 64
PsiI TTA'TAA
Cuts at: 0 518 1318 1977
Size: 518 800 659
Fragments arranged by size:
800 659 518
PspOMI G'GGCC_C
Cuts at: 0 684 1977
Size: 684 1293
PvuII CAG'CTG
Cuts at: 0 561 1977
Size: 561 1416
SacI G_AGCT'C
Cuts at: 0 681 1977
Size: 681 1296
SalI G'TCGA_C
Cuts at: 0 1807 1977
Size: 1807 170
ScaI AGT'ACT
Cuts at: 0 19 1190 1622 1903 1977
Size: 19 1171 432 281 74
Fragments arranged by size:
1171 432 281 74 19
SexAI A'CCwGG_T
Cuts at: 0 1281 1977
Size: 1281 696
SfoI GGC'GCC
Cuts at: 0 88 1977
Size: 88 1889
SmlI C'TyrA_G
Cuts at: 0 192 1036 1103 1977
Size: 192 844 67 874
Fragments arranged by size:
874 844 192 67
StyI C'CwwG_G
Cuts at: 0 1545 1977
Size: 1545 432
SwaI ATTT'AAAT
Cuts at: 0 1202 1977
Size: 1202 775
TatI w'GTAC_w
Cuts at: 0 17 1042 1188 1260 1620 1901 1977
Size: 17 1025 146 72 360 281 76
Fragments arranged by size:
1025 360 281 146 76 72 17
Tth111I GACn'n_nGTC
Cuts at: 0 1642 1977
Size: 1642 335
XbaI T'CTAG_A
Cuts at: 0 1059 1977
Size: 1059 918
XmnI GAAnn'nnTTC
Cuts at: 0 361 1437 1977
Size: 361 1076 540
Fragments arranged by size:
1076 540 361
ZraI GAC'GTC
Cuts at: 0 1486 1977
Size: 1486 491
Enzymes that do cut:
AatII AccI AcuI AlwNI ApaI ApoI AseI BanI
BanII BbeI BbsI BclI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BstBI BstEII BtgZI CspCI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MslI MspA1I NarI NdeI
PsiI PspOMI PvuII SacI SalI ScaI SexAI SfoI
SmlI StyI SwaI TatI Tth111I XbaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmtI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI DraIII DrdI EagI
EciI EcoRV FseI FspI FspAI KpnI MluI MmeI
MscI NaeI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII
SacII SanDI SapI SbfI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI TaqII
TstI XcmI XhoI XmaI