Restriction map for
pF1KB6176
(Linear) (Six-base) MAPSORT of: KIBB6176.seq Check: 2026 from: 1 to: 1818
KIBB6176.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1056 1187 1818
Size: 1056 131 631
Fragments arranged by size:
1056 631 131
AatII G_ACGT'C
Cuts at: 0 1475 1818
Size: 1475 343
AccI GT'mk_AC
Cuts at: 0 68 122 182 1818
Size: 68 54 60 1636
Fragments arranged by size:
1636 68 60 54
Acc65I G'GTAC_C
Cuts at: 0 884 912 1818
Size: 884 28 906
Fragments arranged by size:
906 884 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 303 958 1818
Size: 166 137 655 860
Fragments arranged by size:
860 655 166 137
AflII C'TTAA_G
Cuts at: 0 346 1818
Size: 346 1472
AflIII A'CryG_T
Cuts at: 0 1086 1818
Size: 1086 732
AleI CACnn'nnGTG
Cuts at: 0 94 1149 1818
Size: 94 1055 669
Fragments arranged by size:
1055 669 94
AlwNI CAG_nnn'CTG
Cuts at: 0 936 1405 1610 1689 1818
Size: 936 469 205 79 129
Fragments arranged by size:
936 469 205 129 79
ApaI G_GGCC'C
Cuts at: 0 19 1380 1818
Size: 19 1361 438
Fragments arranged by size:
1361 438 19
AvaI C'yCGr_G
Cuts at: 0 44 761 792 1818
Size: 44 717 31 1026
Fragments arranged by size:
1026 717 44 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 426 459 1818
Size: 426 33 1359
Fragments arranged by size:
1359 426 33
BanI G'GyrC_C
Cuts at: 0 55 884 912 964 1628 1818
Size: 55 829 28 52 664 190
Fragments arranged by size:
829 664 190 55 52 28
BanII G_rGCy'C
Cuts at: 0 19 257 772 958 1162 1322 1380 1818
Size: 19 238 515 186 204 160 58 438
Fragments arranged by size:
515 438 238 204 186 160 58 19
BbeI G_GCGC'C
Cuts at: 0 1632 1818
Size: 1632 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 316 1084 1366 1818
Size: 316 768 282 452
Fragments arranged by size:
768 452 316 282
BbvCI CC'TCA_GC
Cuts at: 0 1424 1591 1818
Size: 1424 167 227
Fragments arranged by size:
1424 227 167
BglI GCCn_nnn'nGGC
Cuts at: 0 31 1535 1818
Size: 31 1504 283
Fragments arranged by size:
1504 283 31
BglII A'GATC_T
Cuts at: 0 1284 1818
Size: 1284 534
BlpI GC'TnA_GC
Cuts at: 0 1034 1818
Size: 1034 784
Bme1580I G_kGCm'C
Cuts at: 0 19 58 1380 1818
Size: 19 39 1322 438
Fragments arranged by size:
1322 438 39 19
BmgBI CAC'GTC
Cuts at: 0 638 1224 1818
Size: 638 586 594
Fragments arranged by size:
638 594 586
BmrI ACTGGGnnnn_n'
Cuts at: 0 914 915 1818
Size: 914 1 903
Fragments arranged by size:
914 903 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 528 1413 1818
Size: 528 885 405
Fragments arranged by size:
885 528 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 1146 1587 1818
Size: 477 669 441 231
Fragments arranged by size:
669 477 441 231
Bpu10I CC'TnA_GC
Cuts at: 0 1072 1424 1591 1818
Size: 1072 352 167 227
Fragments arranged by size:
1072 352 227 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1273 1633 1818
Size: 997 276 360 185
Fragments arranged by size:
997 360 276 185
BsaHI Gr'CG_yC
Cuts at: 0 1472 1629 1818
Size: 1472 157 189
Fragments arranged by size:
1472 189 157
BsaWI w'CCGG_w
Cuts at: 0 484 1818
Size: 484 1334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 627 657 1703 1733 1818
Size: 627 30 1046 30 85
Fragments arranged by size:
1046 627 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 755 1678 1818
Size: 265 490 923 140
Fragments arranged by size:
923 490 265 140
BseYI C'CCAG_C
Cuts at: 0 553 566 603 772 777 1022 1379 1818
Size: 553 13 37 169 5 245 357 439
Fragments arranged by size:
553 439 357 245 169 37 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1818
Size: 949 869
BsiEI CG_ry'CG
Cuts at: 0 1126 1818
Size: 1126 692
BsiHKAI G_wGCw'C
Cuts at: 0 257 587 1013 1073 1496 1818
Size: 257 330 426 60 423 322
Fragments arranged by size:
426 423 330 322 257 60
Bsp1286I G_dGCh'C
Cuts at: 0 19 58 257 587 772 958 1013 1073
Size: 19 39 199 330 185 186 55 60
Cuts at: 1073 1162 1322 1380 1496 1818
Size: 89 160 58 116 322
Fragments arranged by size:
330 322 199 186 185 160 116 89
60 58 55 39 19
BspEI T'CCGG_A
Cuts at: 0 484 1818
Size: 484 1334
BspHI T'CATG_A
Cuts at: 0 1115 1818
Size: 1115 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 1056 1187 1818
Size: 80 976 131 631
Fragments arranged by size:
976 631 131 80
BsrBI CCG'CTC
Cuts at: 0 632 840 1373 1668 1734 1818
Size: 632 208 533 295 66 84
Fragments arranged by size:
632 533 295 208 84 66
BsrDI GCAATG_nn'
Cuts at: 0 230 1818
Size: 230 1588
BsrFI r'CCGG_y
Cuts at: 0 730 1818
Size: 730 1088
BstXI CCAn_nnnn'nTGG
Cuts at: 0 382 703 1818
Size: 382 321 1115
Fragments arranged by size:
1115 382 321
BstYI r'GATC_y
Cuts at: 0 1284 1402 1685 1818
Size: 1284 118 283 133
Fragments arranged by size:
1284 283 133 118
BstZ17I GTA'TAC
Cuts at: 0 69 1818
Size: 69 1749
Bsu36I CC'TnA_GG
Cuts at: 0 1398 1818
Size: 1398 420
BtgI C'CryG_G
Cuts at: 0 10 360 668 677 1569 1818
Size: 10 350 308 9 892 249
Fragments arranged by size:
892 350 308 249 10 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1007 1818
Size: 1007 811
BtsI GCAGTG_nn'
Cuts at: 0 56 212 1516 1818
Size: 56 156 1304 302
Fragments arranged by size:
1304 302 156 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 385 420 1818
Size: 385 35 1398
Fragments arranged by size:
1398 385 35
DraIII CAC_nnn'GTG
Cuts at: 0 922 1148 1818
Size: 922 226 670
Fragments arranged by size:
922 670 226
EaeI y'GGCC_r
Cuts at: 0 705 851 998 1123 1187 1349 1691 1818
Size: 705 146 147 125 64 162 342 127
Fragments arranged by size:
705 342 162 147 146 127 125 64
EagI C'GGCC_G
Cuts at: 0 1123 1818
Size: 1123 695
EarI CTCTTCn'nnn_
Cuts at: 0 934 1681 1818
Size: 934 747 137
Fragments arranged by size:
934 747 137
EcoICRI GAG'CTC
Cuts at: 0 255 1818
Size: 255 1563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 303 477 958 1146 1587 1818
Size: 166 137 174 481 188 441 231
Fragments arranged by size:
481 441 231 188 174 166 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 795 1312 1818
Size: 795 517 506
Fragments arranged by size:
795 517 506
EcoO109I rG'GnC_Cy
Cuts at: 0 15 16 141 267 417 550 1376 1443
Size: 15 1 125 126 150 133 826 67
Cuts at: 1443 1818
Size: 375
Fragments arranged by size:
826 375 150 133 126 125 67 15
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1818
Size: 333 1485
HaeII r_GCGC'y
Cuts at: 0 1632 1818
Size: 1632 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 829 861 1121 1153 1276 1308 1818
Size: 829 32 260 32 123 32 510
Fragments arranged by size:
829 510 260 123 32 32 32
HincII GTy'rAC
Cuts at: 0 435 1092 1818
Size: 435 657 726
Fragments arranged by size:
726 657 435
KasI G'GCGC_C
Cuts at: 0 1628 1818
Size: 1628 190
KpnI G_GTAC'C
Cuts at: 0 888 916 1818
Size: 888 28 902
Fragments arranged by size:
902 888 28
MfeI C'AATT_G
Cuts at: 0 1697 1818
Size: 1697 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1818
Size: 710 1108
MscI TGG'CCA
Cuts at: 0 707 1351 1693 1818
Size: 707 644 342 125
Fragments arranged by size:
707 644 342 125
MslI CAynn'nnrTG
Cuts at: 0 94 992 1149 1181 1222 1818
Size: 94 898 157 32 41 596
Fragments arranged by size:
898 596 157 94 41 32
MspA1I CmG'CkG
Cuts at: 0 285 933 1033 1423 1659 1818
Size: 285 648 100 390 236 159
Fragments arranged by size:
648 390 285 236 159 100
NarI GG'CG_CC
Cuts at: 0 1629 1818
Size: 1629 189
NcoI C'CATG_G
Cuts at: 0 360 677 1818
Size: 360 317 1141
Fragments arranged by size:
1141 360 317
NdeI CA'TA_TG
Cuts at: 0 656 1818
Size: 656 1162
NspI r_CATG'y
Cuts at: 0 1090 1186 1818
Size: 1090 96 632
Fragments arranged by size:
1090 632 96
PasI CC'CwG_GG
Cuts at: 0 546 1242 1818
Size: 546 696 576
Fragments arranged by size:
696 576 546
PciI A'CATG_T
Cuts at: 0 1086 1818
Size: 1086 732
PflMI CCAn_nnn'nTGG
Cuts at: 0 1248 1386 1689 1818
Size: 1248 138 303 129
Fragments arranged by size:
1248 303 138 129
PfoI T'CCnGG_A
Cuts at: 0 104 473 1818
Size: 104 369 1345
Fragments arranged by size:
1345 369 104
PpuMI rG'GwC_Cy
Cuts at: 0 267 417 550 1443 1818
Size: 267 150 133 893 375
Fragments arranged by size:
893 375 267 150 133
PspOMI G'GGCC_C
Cuts at: 0 15 1376 1818
Size: 15 1361 442
Fragments arranged by size:
1361 442 15
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 868 900 1818
Size: 868 32 918
Fragments arranged by size:
918 868 32
PvuII CAG'CTG
Cuts at: 0 933 1033 1423 1818
Size: 933 100 390 395
Fragments arranged by size:
933 395 390 100
SacI G_AGCT'C
Cuts at: 0 257 1818
Size: 257 1561
SanDI GG'GwC_CC
Cuts at: 0 550 1818
Size: 550 1268
SfcI C'TryA_G
Cuts at: 0 619 1455 1818
Size: 619 836 363
Fragments arranged by size:
836 619 363
SfoI GGC'GCC
Cuts at: 0 1630 1818
Size: 1630 188
SmaI CCC'GGG
Cuts at: 0 46 763 1818
Size: 46 717 1055
Fragments arranged by size:
1055 717 46
SmlI C'TyrA_G
Cuts at: 0 346 1012 1288 1648 1818
Size: 346 666 276 360 170
Fragments arranged by size:
666 360 346 276 170
StyI C'CwwG_G
Cuts at: 0 360 413 677 848 1385 1616 1818
Size: 360 53 264 171 537 231 202
Fragments arranged by size:
537 360 264 231 202 171 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 580 933 1780 1818
Size: 580 353 847 38
Fragments arranged by size:
847 580 353 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 1818
Size: 627 32 1159
Fragments arranged by size:
1159 627 32
Tth111I GACn'n_nGTC
Cuts at: 0 1207 1818
Size: 1207 611
XbaI T'CTAG_A
Cuts at: 0 132 1818
Size: 132 1686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 654 675 1818
Size: 237 417 21 1143
Fragments arranged by size:
1143 417 237 21
XmaI C'CCGG_G
Cuts at: 0 44 761 1818
Size: 44 717 1057
Fragments arranged by size:
1057 717 44
XmnI GAAnn'nnTTC
Cuts at: 0 313 1818
Size: 313 1505
ZraI GAC'GTC
Cuts at: 0 1473 1818
Size: 1473 345
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AleI
AlwNI ApaI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PsrI
PvuII SacI SanDI SfcI SfoI SmaI SmlI StyI
TaqII TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BcgI BciVI BclI
BfrBI BmtI BsaI BsaAI BsaBI BsiWI BsmI BsmBI
BsrGI BssHII BssSI BstAPI BstBI BstEII ClaI DraI
DrdI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XhoI