Restriction map for
pF1KB6169
(Linear) (Six-base) MAPSORT of: KIBB6169.seq Check: 6009 from: 1 to: 1950
KIBB6169.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 434 1950
Size: 434 1516
Acc65I G'GTAC_C
Cuts at: 0 1052 1950
Size: 1052 898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1647 1950
Size: 1175 472 303
Fragments arranged by size:
1175 472 303
AhdI GACnn_n'nnGTC
Cuts at: 0 1665 1695 1950
Size: 1665 30 255
Fragments arranged by size:
1665 255 30
AlwNI CAG_nnn'CTG
Cuts at: 0 256 1950
Size: 256 1694
ApaI G_GGCC'C
Cuts at: 0 767 1039 1348 1473 1950
Size: 767 272 309 125 477
Fragments arranged by size:
767 477 309 272 125
ApaLI G'TGCA_C
Cuts at: 0 58 246 1950
Size: 58 188 1704
Fragments arranged by size:
1704 188 58
AvaI C'yCGr_G
Cuts at: 0 913 1340 1466 1620 1640 1670 1715 1724
Size: 913 427 126 154 20 30 45 9
Cuts at: 1724 1895 1950
Size: 171 55
Fragments arranged by size:
913 427 171 154 126 55 45 30
20 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1695 1728 1950
Size: 1695 33 222
Fragments arranged by size:
1695 222 33
BanI G'GyrC_C
Cuts at: 0 842 881 1052 1950
Size: 842 39 171 898
Fragments arranged by size:
898 842 171 39
BanII G_rGCy'C
Cuts at: 0 167 184 707 743 767 1039 1348 1424
Size: 167 17 523 36 24 272 309 76
Cuts at: 1424 1473 1950
Size: 49 477
Fragments arranged by size:
523 477 309 272 167 76 49 36
24 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 779 1950
Size: 210 569 1171
Fragments arranged by size:
1171 569 210
BciVI GTATCCnnnnn_n'
Cuts at: 0 1057 1950
Size: 1057 893
BglI GCCn_nnn'nGGC
Cuts at: 0 136 145 457 1950
Size: 136 9 312 1493
Fragments arranged by size:
1493 312 136 9
BlpI GC'TnA_GC
Cuts at: 0 235 737 1092 1950
Size: 235 502 355 858
Fragments arranged by size:
858 502 355 235
Bme1580I G_kGCm'C
Cuts at: 0 62 250 767 886 1039 1348 1473 1950
Size: 62 188 517 119 153 309 125 477
Fragments arranged by size:
517 477 309 188 153 125 119 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 1262 1950
Size: 1262 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1550 1899 1950
Size: 1550 349 51
Fragments arranged by size:
1550 349 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 661 1079 1091 1722 1795 1950
Size: 153 508 418 12 631 73 155
Fragments arranged by size:
631 508 418 155 153 73 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 1950
Size: 1894 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 666 1538 1673 1809 1870 1950
Size: 666 872 135 136 61 80
Fragments arranged by size:
872 666 136 135 80 61
BsaAI yAC'GTr
Cuts at: 0 297 1950
Size: 297 1653
BsaBI GATnn'nnATC
Cuts at: 0 586 1950
Size: 586 1364
BsaHI Gr'CG_yC
Cuts at: 0 1484 1891 1950
Size: 1484 407 59
Fragments arranged by size:
1484 407 59
BsaWI w'CCGG_w
Cuts at: 0 592 1950
Size: 592 1358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1720 1750 1950
Size: 1720 30 200
Fragments arranged by size:
1720 200 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 66 814 1387 1569 1792 1819 1824 1827
Size: 66 748 573 182 223 27 5 3
Cuts at: 1827 1843 1846 1950
Size: 16 3 104
Fragments arranged by size:
748 573 223 182 104 66 27 16
5 3 3
BseYI C'CCAG_C
Cuts at: 0 177 452 958 973 1950
Size: 177 275 506 15 977
Fragments arranged by size:
977 506 275 177 15
BsiEI CG_ry'CG
Cuts at: 0 140 194 215 1950
Size: 140 54 21 1735
Fragments arranged by size:
1735 140 54 21
BsiHKAI G_wGCw'C
Cuts at: 0 62 250 707 743 1513 1950
Size: 62 188 457 36 770 437
Fragments arranged by size:
770 457 437 188 62 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1067 1950
Size: 1067 883
Bsp1286I G_dGCh'C
Cuts at: 0 62 167 184 250 707 743 767 886
Size: 62 105 17 66 457 36 24 119
Cuts at: 886 1039 1348 1424 1473 1513 1950
Size: 153 309 76 49 40 437
Fragments arranged by size:
457 437 309 153 119 105 76 66
62 49 40 36 24 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1520 1950
Size: 1520 430
BsrBI CCG'CTC
Cuts at: 0 1284 1475 1950
Size: 1284 191 475
Fragments arranged by size:
1284 475 191
BsrFI r'CCGG_y
Cuts at: 0 157 931 1950
Size: 157 774 1019
Fragments arranged by size:
1019 774 157
BssHII G'CGCG_C
Cuts at: 0 80 1950
Size: 80 1870
BssSI C'ACGA_G
Cuts at: 0 1312 1539 1950
Size: 1312 227 411
Fragments arranged by size:
1312 411 227
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 1950
Size: 245 1705
BstEII G'GTnAC_C
Cuts at: 0 273 1950
Size: 273 1677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1950
Size: 177 1773
Bsu36I CC'TnA_GG
Cuts at: 0 94 726 948 1950
Size: 94 632 222 1002
Fragments arranged by size:
1002 632 222 94
BtgI C'CryG_G
Cuts at: 0 656 1031 1478 1579 1950
Size: 656 375 447 101 371
Fragments arranged by size:
656 447 375 371 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 157 390 1950
Size: 157 233 1560
Fragments arranged by size:
1560 233 157
BtsI GCAGTG_nn'
Cuts at: 0 1149 1170 1950
Size: 1149 21 780
Fragments arranged by size:
1149 780 21
DraIII CAC_nnn'GTG
Cuts at: 0 245 300 1950
Size: 245 55 1650
Fragments arranged by size:
1650 245 55
DrdI GACnn_nn'nnGTC
Cuts at: 0 214 1950
Size: 214 1736
EaeI y'GGCC_r
Cuts at: 0 137 155 191 425 527 1101 1235 1272
Size: 137 18 36 234 102 574 134 37
Cuts at: 1272 1383 1950
Size: 111 567
Fragments arranged by size:
574 567 234 137 134 111 102 37
36 18
EagI C'GGCC_G
Cuts at: 0 137 191 1950
Size: 137 54 1759
Fragments arranged by size:
1759 137 54
EarI CTCTTCn'nnn_
Cuts at: 0 313 787 1832 1950
Size: 313 474 1045 118
Fragments arranged by size:
1045 474 313 118
EcoICRI GAG'CTC
Cuts at: 0 705 741 1950
Size: 705 36 1209
Fragments arranged by size:
1209 705 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 661 1079 1091 1175 1647 1722 1795
Size: 153 508 418 12 84 472 75 73
Cuts at: 1795 1950
Size: 155
Fragments arranged by size:
508 472 418 155 153 84 75 73
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1312 1950
Size: 1312 638
EcoO109I rG'GnC_Cy
Cuts at: 0 672 722 763 918 1036 1209 1344 1345
Size: 672 50 41 155 118 173 135 1
Cuts at: 1345 1644 1697 1719 1749 1950
Size: 299 53 22 30 201
Fragments arranged by size:
672 299 201 173 155 135 118 53
50 41 30 22 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1935 1950
Size: 1935 15
FspI TGC'GCA
Cuts at: 0 511 1950
Size: 511 1439
FspAI rTGC'GCAy
Cuts at: 0 511 1950
Size: 511 1439
HaeII r_GCGC'y
Cuts at: 0 151 1680 1950
Size: 151 1529 270
Fragments arranged by size:
1529 270 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 1312 1344 1651 1681 1683 1713
Size: 403 32 877 32 307 30 2 30
Cuts at: 1713 1720 1752 1950
Size: 7 32 198
Fragments arranged by size:
877 403 307 198 32 32 32 30
30 7 2
HincII GTy'rAC
Cuts at: 0 435 1080 1950
Size: 435 645 870
Fragments arranged by size:
870 645 435
KpnI G_GTAC'C
Cuts at: 0 1056 1950
Size: 1056 894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1781 1950
Size: 308 1473 169
Fragments arranged by size:
1473 308 169
MscI TGG'CCA
Cuts at: 0 529 1103 1237 1385 1950
Size: 529 574 134 148 565
Fragments arranged by size:
574 565 529 148 134
MslI CAynn'nnrTG
Cuts at: 0 271 1950
Size: 271 1679
MspA1I CmG'CkG
Cuts at: 0 456 1096 1581 1950
Size: 456 640 485 369
Fragments arranged by size:
640 485 456 369
NaeI GCC'GGC
Cuts at: 0 159 933 1950
Size: 159 774 1017
Fragments arranged by size:
1017 774 159
NcoI C'CATG_G
Cuts at: 0 1031 1950
Size: 1031 919
NgoMIV G'CCGG_C
Cuts at: 0 157 931 1950
Size: 157 774 1019
Fragments arranged by size:
1019 774 157
NspI r_CATG'y
Cuts at: 0 1575 1950
Size: 1575 375
PasI CC'CwG_GG
Cuts at: 0 555 1403 1950
Size: 555 848 547
Fragments arranged by size:
848 555 547
PflMI CCAn_nnn'nTGG
Cuts at: 0 1409 1950
Size: 1409 541
PfoI T'CCnGG_A
Cuts at: 0 1364 1950
Size: 1364 586
PmlI CAC'GTG
Cuts at: 0 297 1950
Size: 297 1653
PpuMI rG'GwC_Cy
Cuts at: 0 672 722 1209 1644 1697 1719 1749 1950
Size: 672 50 487 435 53 22 30 201
Fragments arranged by size:
672 487 435 201 53 50 30 22
PspOMI G'GGCC_C
Cuts at: 0 763 1035 1344 1469 1950
Size: 763 272 309 125 481
Fragments arranged by size:
763 481 309 272 125
PspXI vC'TCGA_Gb
Cuts at: 0 1620 1950
Size: 1620 330
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 460 492 1950
Size: 460 32 1458
Fragments arranged by size:
1458 460 32
PvuII CAG'CTG
Cuts at: 0 456 1096 1950
Size: 456 640 854
Fragments arranged by size:
854 640 456
SacI G_AGCT'C
Cuts at: 0 707 743 1950
Size: 707 36 1207
Fragments arranged by size:
1207 707 36
SacII CC_GC'GG
Cuts at: 0 1582 1950
Size: 1582 368
SalI G'TCGA_C
Cuts at: 0 433 1950
Size: 433 1517
SanDI GG'GwC_CC
Cuts at: 0 1644 1719 1950
Size: 1644 75 231
Fragments arranged by size:
1644 231 75
SapI GCTCTTCn'nnn_
Cuts at: 0 787 1950
Size: 787 1163
SexAI A'CCwGG_T
Cuts at: 0 241 747 1950
Size: 241 506 1203
Fragments arranged by size:
1203 506 241
SfcI C'TryA_G
Cuts at: 0 1170 1950
Size: 1170 780
SmaI CCC'GGG
Cuts at: 0 1342 1468 1642 1717 1950
Size: 1342 126 174 75 233
Fragments arranged by size:
1342 233 174 126 75
SmlI C'TyrA_G
Cuts at: 0 1620 1909 1950
Size: 1620 289 41
Fragments arranged by size:
1620 289 41
StuI AGG'CCT
Cuts at: 0 694 1843 1950
Size: 694 1149 107
Fragments arranged by size:
1149 694 107
StyI C'CwwG_G
Cuts at: 0 73 714 1031 1104 1950
Size: 73 641 317 73 846
Fragments arranged by size:
846 641 317 73 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1894 1950
Size: 1894 56
TatI w'GTAC_w
Cuts at: 0 380 1214 1950
Size: 380 834 736
Fragments arranged by size:
834 736 380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 24 56 1360 1392 1950
Size: 24 32 1304 32 558
Fragments arranged by size:
1304 558 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 211 279 1950
Size: 211 68 1671
Fragments arranged by size:
1671 211 68
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 1950
Size: 1275 675
XhoI C'TCGA_G
Cuts at: 0 1620 1950
Size: 1620 330
XmaI C'CCGG_G
Cuts at: 0 1340 1466 1640 1715 1950
Size: 1340 126 174 75 235
Fragments arranged by size:
1340 235 174 126 75
Enzymes that do cut:
AccI Acc65I AcuI AhdI AlwNI ApaI ApaLI AvaI
BaeI BanI BanII BbsI BciVI BglI BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII SacI SacII SalI
SanDI SapI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BclI BfrBI BglII BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BstBI BstYI BstZ17I ClaI CspCI DraI EciI EcoRI
EcoRV FseI HindIII HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PstI PvuI RsrII SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XmnI ZraI