Restriction map for
pF1KB6140
(Linear) (Six-base) MAPSORT of: KIBB6140.seq Check: 3375 from: 1 to: 1902
KIBB6140.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 434 463 614 961 1396 1516 1902
Size: 95 339 29 151 347 435 120 386
Fragments arranged by size:
435 386 347 339 151 120 95 29
AfeI AGC'GCT
Cuts at: 0 41 1902
Size: 41 1861
AflIII A'CryG_T
Cuts at: 0 1247 1631 1902
Size: 1247 384 271
Fragments arranged by size:
1247 384 271
AhdI GACnn_n'nnGTC
Cuts at: 0 276 1902
Size: 276 1626
AleI CACnn'nnGTG
Cuts at: 0 988 1902
Size: 988 914
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1902
Size: 544 1358
ApaI G_GGCC'C
Cuts at: 0 245 1902
Size: 245 1657
ApaLI G'TGCA_C
Cuts at: 0 1253 1810 1902
Size: 1253 557 92
Fragments arranged by size:
1253 557 92
AvaI C'yCGr_G
Cuts at: 0 336 342 815 1081 1235 1595 1616 1902
Size: 336 6 473 266 154 360 21 286
Fragments arranged by size:
473 360 336 286 266 154 21 6
BanI G'GyrC_C
Cuts at: 0 825 1303 1902
Size: 825 478 599
Fragments arranged by size:
825 599 478
BanII G_rGCy'C
Cuts at: 0 245 343 718 753 1244 1472 1822 1902
Size: 245 98 375 35 491 228 350 80
Fragments arranged by size:
491 375 350 245 228 98 80 35
BbeI G_GCGC'C
Cuts at: 0 1307 1902
Size: 1307 595
BbsI GAAGACnn'nnnn_
Cuts at: 0 1078 1762 1902
Size: 1078 684 140
Fragments arranged by size:
1078 684 140
BbvCI CC'TCA_GC
Cuts at: 0 181 1765 1902
Size: 181 1584 137
Fragments arranged by size:
1584 181 137
BclI T'GATC_A
Cuts at: 0 431 1902
Size: 431 1471
BglI GCCn_nnn'nGGC
Cuts at: 0 817 1048 1902
Size: 817 231 854
Fragments arranged by size:
854 817 231
BlpI GC'TnA_GC
Cuts at: 0 752 1902
Size: 752 1150
Bme1580I G_kGCm'C
Cuts at: 0 245 589 830 1257 1444 1814 1902
Size: 245 344 241 427 187 370 88
Fragments arranged by size:
427 370 344 245 241 187 88
BmgBI CAC'GTC
Cuts at: 0 306 1902
Size: 306 1596
BmrI ACTGGGnnnn_n'
Cuts at: 0 824 1878 1902
Size: 824 1054 24
Fragments arranged by size:
1054 824 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 1867 1902
Size: 351 1516 35
Fragments arranged by size:
1516 351 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1004 1087 1189 1213 1566 1575 1902
Size: 604 400 83 102 24 353 9 327
Fragments arranged by size:
604 400 353 327 102 83 24 9
Bpu10I CC'TnA_GC
Cuts at: 0 149 181 965 1536 1765 1902
Size: 149 32 784 571 229 137
Fragments arranged by size:
784 571 229 149 137 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1902
Size: 1336 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 282 1902
Size: 282 1620
BsaAI yAC'GTr
Cuts at: 0 1277 1895 1902
Size: 1277 618 7
Fragments arranged by size:
1277 618 7
BsaHI Gr'CG_yC
Cuts at: 0 688 820 1304 1612 1759 1902
Size: 688 132 484 308 147 143
Fragments arranged by size:
688 484 308 147 143 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 552 582 785 815 1804 1834 1902
Size: 552 30 203 30 989 30 68
Fragments arranged by size:
989 552 203 68 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 300 793 1186 1306 1812 1902
Size: 300 493 393 120 506 90
Fragments arranged by size:
506 493 393 300 120 90
BseYI C'CCAG_C
Cuts at: 0 699 930 1104 1129 1902
Size: 699 231 174 25 773
Fragments arranged by size:
773 699 231 174 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1201 1436 1612 1902
Size: 856 345 235 176 290
Fragments arranged by size:
856 345 290 235 176
BsiEI CG_ry'CG
Cuts at: 0 1040 1220 1829 1902
Size: 1040 180 609 73
Fragments arranged by size:
1040 609 180 73
BsiHKAI G_wGCw'C
Cuts at: 0 317 556 718 1257 1364 1814 1822 1902
Size: 317 239 162 539 107 450 8 80
Fragments arranged by size:
539 450 317 239 162 107 80 8
Bsp1286I G_dGCh'C
Cuts at: 0 245 317 343 556 589 718 753 830
Size: 245 72 26 213 33 129 35 77
Cuts at: 830 1244 1257 1364 1444 1472 1814 1822 1902
Size: 414 13 107 80 28 342 8 80
Fragments arranged by size:
414 342 245 213 129 107 80 80
77 72 35 33 28 26 13 8
BspHI T'CATG_A
Cuts at: 0 398 1902
Size: 398 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 151 1491 1902
Size: 151 1340 411
Fragments arranged by size:
1340 411 151
BsrBI CCG'CTC
Cuts at: 0 964 1301 1535 1874 1902
Size: 964 337 234 339 28
Fragments arranged by size:
964 339 337 234 28
BsrFI r'CCGG_y
Cuts at: 0 1370 1693 1723 1902
Size: 1370 323 30 179
Fragments arranged by size:
1370 323 179 30
BssSI C'ACGA_G
Cuts at: 0 475 1902
Size: 475 1427
BstEII G'GTnAC_C
Cuts at: 0 1002 1902
Size: 1002 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 210 990 1680 1902
Size: 204 6 780 690 222
Fragments arranged by size:
780 690 222 204 6
BstYI r'GATC_y
Cuts at: 0 1085 1902
Size: 1085 817
BtgI C'CryG_G
Cuts at: 0 197 203 209 245 362 1829 1902
Size: 197 6 6 36 117 1467 73
Fragments arranged by size:
1467 197 117 73 36 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 251 1902
Size: 251 1651
BtsI GCAGTG_nn'
Cuts at: 0 654 1902
Size: 654 1248
DraI TTT'AAA
Cuts at: 0 119 1902
Size: 119 1783
DraIII CAC_nnn'GTG
Cuts at: 0 268 1491 1902
Size: 268 1223 411
Fragments arranged by size:
1223 411 268
EaeI y'GGCC_r
Cuts at: 0 200 206 1037 1217 1604 1670 1826 1902
Size: 200 6 831 180 387 66 156 76
Fragments arranged by size:
831 387 200 180 156 76 66 6
EagI C'GGCC_G
Cuts at: 0 1037 1217 1826 1902
Size: 1037 180 609 76
Fragments arranged by size:
1037 609 180 76
EarI CTCTTCn'nnn_
Cuts at: 0 633 1291 1902
Size: 633 658 611
Fragments arranged by size:
658 633 611
EcoICRI GAG'CTC
Cuts at: 0 716 1820 1902
Size: 716 1104 82
Fragments arranged by size:
1104 716 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 434 463 604 614 961 1004 1087
Size: 95 339 29 141 10 347 43 83
Cuts at: 1087 1189 1213 1396 1516 1566 1575 1902
Size: 102 24 183 120 50 9 327
Fragments arranged by size:
347 339 327 183 141 120 102 95
83 50 43 29 24 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1171 1435 1902
Size: 1171 264 467
Fragments arranged by size:
1171 467 264
EcoO109I rG'GnC_Cy
Cuts at: 0 51 393 867 1778 1902
Size: 51 342 474 911 124
Fragments arranged by size:
911 474 342 124 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 1401 1902
Size: 1287 114 501
Fragments arranged by size:
1287 501 114
FspI TGC'GCA
Cuts at: 0 256 1057 1902
Size: 256 801 845
Fragments arranged by size:
845 801 256
FspAI rTGC'GCAy
Cuts at: 0 256 1902
Size: 256 1646
HaeII r_GCGC'y
Cuts at: 0 43 1054 1136 1307 1351 1399 1724 1739
Size: 43 1011 82 171 44 48 325 15
Cuts at: 1739 1902
Size: 163
Fragments arranged by size:
1011 325 171 163 82 48 44 43
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 552 584 839 871 1902
Size: 262 32 258 32 255 32 1031
Fragments arranged by size:
1031 262 258 255 32 32 32
HincII GTy'rAC
Cuts at: 0 912 1902
Size: 912 990
KasI G'GCGC_C
Cuts at: 0 1303 1902
Size: 1303 599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 344 1769 1902
Size: 190 154 1425 133
Fragments arranged by size:
1425 190 154 133
MscI TGG'CCA
Cuts at: 0 208 1902
Size: 208 1694
MslI CAynn'nnrTG
Cuts at: 0 139 202 208 988 1902
Size: 139 63 6 780 914
Fragments arranged by size:
914 780 139 63 6
MspA1I CmG'CkG
Cuts at: 0 441 1065 1283 1425 1477 1590 1902
Size: 441 624 218 142 52 113 312
Fragments arranged by size:
624 441 312 218 142 113 52
NaeI GCC'GGC
Cuts at: 0 1372 1725 1902
Size: 1372 353 177
Fragments arranged by size:
1372 353 177
NarI GG'CG_CC
Cuts at: 0 1304 1902
Size: 1304 598
NcoI C'CATG_G
Cuts at: 0 203 209 1902
Size: 203 6 1693
Fragments arranged by size:
1693 203 6
NgoMIV G'CCGG_C
Cuts at: 0 1370 1723 1902
Size: 1370 353 179
Fragments arranged by size:
1370 353 179
NotI GC'GGCC_GC
Cuts at: 0 1037 1902
Size: 1037 865
NspI r_CATG'y
Cuts at: 0 17 255 1251 1902
Size: 17 238 996 651
Fragments arranged by size:
996 651 238 17
PciI A'CATG_T
Cuts at: 0 1247 1902
Size: 1247 655
PfoI T'CCnGG_A
Cuts at: 0 1853 1902
Size: 1853 49
PmlI CAC'GTG
Cuts at: 0 1277 1895 1902
Size: 1277 618 7
Fragments arranged by size:
1277 618 7
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1651 1683 1902
Size: 1651 32 219
Fragments arranged by size:
1651 219 32
PpuMI rG'GwC_Cy
Cuts at: 0 51 1778 1902
Size: 51 1727 124
Fragments arranged by size:
1727 124 51
PspOMI G'GGCC_C
Cuts at: 0 241 1902
Size: 241 1661
PspXI vC'TCGA_Gb
Cuts at: 0 336 1902
Size: 336 1566
PstI C_TGCA'G
Cuts at: 0 1403 1436 1658 1902
Size: 1403 33 222 244
Fragments arranged by size:
1403 244 222 33
PvuII CAG'CTG
Cuts at: 0 441 1065 1590 1902
Size: 441 624 525 312
Fragments arranged by size:
624 525 441 312
SacI G_AGCT'C
Cuts at: 0 718 1822 1902
Size: 718 1104 80
Fragments arranged by size:
1104 718 80
SanDI GG'GwC_CC
Cuts at: 0 51 1778 1902
Size: 51 1727 124
Fragments arranged by size:
1727 124 51
SapI GCTCTTCn'nnn_
Cuts at: 0 1291 1902
Size: 1291 611
SexAI A'CCwGG_T
Cuts at: 0 1157 1902
Size: 1157 745
SfcI C'TryA_G
Cuts at: 0 1399 1432 1573 1654 1902
Size: 1399 33 141 81 248
Fragments arranged by size:
1399 248 141 81 33
SfoI GGC'GCC
Cuts at: 0 1305 1902
Size: 1305 597
SmaI CCC'GGG
Cuts at: 0 817 1237 1597 1902
Size: 817 420 360 305
Fragments arranged by size:
817 420 360 305
SmlI C'TyrA_G
Cuts at: 0 336 1081 1351 1902
Size: 336 745 270 551
Fragments arranged by size:
745 551 336 270
SpeI A'CTAG_T
Cuts at: 0 985 1902
Size: 985 917
SphI G_CATG'C
Cuts at: 0 17 1902
Size: 17 1885
StuI AGG'CCT
Cuts at: 0 1579 1902
Size: 1579 323
StyI C'CwwG_G
Cuts at: 0 171 203 209 1460 1902
Size: 171 32 6 1251 442
Fragments arranged by size:
1251 442 171 32 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 1110 1662 1902
Size: 19 1091 552 240
Fragments arranged by size:
1091 552 240 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 785 817 1261 1293 1623 1655 1804 1836
Size: 785 32 444 32 330 32 149 32
Cuts at: 1836 1902
Size: 66
Fragments arranged by size:
785 444 330 149 66 32 32 32
32
XhoI C'TCGA_G
Cuts at: 0 336 1081 1902
Size: 336 745 821
Fragments arranged by size:
821 745 336
XmaI C'CCGG_G
Cuts at: 0 815 1235 1595 1902
Size: 815 420 360 307
Fragments arranged by size:
815 420 360 307
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SanDI SapI SexAI SfcI SfoI SmaI SmlI
SpeI SphI StuI StyI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BfrBI BglII BmtI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I ClaI CspCI DrdI EciI EcoRI EcoRV
FseI HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PasI PflMI PmeI PshAI PsiI
PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI
SgfI SgrAI SnaBI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XmnI ZraI