(Linear) (Six-base) MAPSORT of: KIBB6120.seq  Check: 672  from: 1  to: 1749



KIBB6120.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0     149    1749 
   Size:        149    1600
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     595    1208    1502    1567    1749 
   Size:        278     317     613     294      65     182

  Fragments arranged by size: 

                 613     317     294     278     182      65
 
AflIII A'CryG_T

Cuts at:      0    1539    1749 
   Size:       1539     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361    1749 
   Size:        361    1388
 
ApoI r'AATT_y

Cuts at:      0      49     247     760    1267    1749 
   Size:         49     198     513     507     482

  Fragments arranged by size: 

                 513     507     482     198      49
 
AseI AT'TA_AT

Cuts at:      0     277    1749 
   Size:        277    1472
 
BanI G'GyrC_C

Cuts at:      0     789    1749 
   Size:        789     960
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1367    1749 
   Size:       1367     382
 
BglII A'GATC_T

Cuts at:      0     588    1749 
   Size:        588    1161
 
BlpI GC'TnA_GC

Cuts at:      0      41    1749 
   Size:         41    1708
 
Bme1580I G_kGCm'C

Cuts at:      0     963    1749 
   Size:        963     786
 
BmtI G_CTAG'C

Cuts at:      0    1252    1749 
   Size:       1252     497
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     744    1749 
   Size:        744    1005
 
Bpu10I CC'TnA_GC

Cuts at:      0     920    1749 
   Size:        920     829
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     946    1749 
   Size:        946     803
 
BseYI C'CCAG_C

Cuts at:      0     461    1749 
   Size:        461    1288
 
Bsp1286I G_dGCh'C

Cuts at:      0     963    1749 
   Size:        963     786
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1201    1261    1749 
   Size:       1201      60     488

  Fragments arranged by size: 

                1201     488      60
 
BstYI r'GATC_y

Cuts at:      0     588    1749 
   Size:        588    1161
 
BtsI GCAGTG_nn'

Cuts at:      0    1271    1749 
   Size:       1271     478
 
ClaI AT'CG_AT

Cuts at:      0    1730    1749 
   Size:       1730      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     307    1028    1058    1184    1310    1376    1749 
   Size:        307     721      30     126     126      66     373

  Fragments arranged by size: 

                 721     373     307     126     126      66      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     595     744    1208    1502    1567    1749 
   Size:        278     317     149     464     294      65     182

  Fragments arranged by size: 

                 464     317     294     278     182     149      65
 
EcoRI G'AATT_C

Cuts at:      0    1267    1749 
   Size:       1267     482
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     903     935    1183    1215    1221    1253    1749 
   Size:        903      32     248      32       6      32     496

  Fragments arranged by size: 

                 903     496     248      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0     609     701    1749 
   Size:        609      92    1048

  Fragments arranged by size: 

                1048     609      92
 
HindIII A'AGCT_T

Cuts at:      0    1343    1749 
   Size:       1343     406
 
HpaI GTT'AAC

Cuts at:      0     701    1749 
   Size:        701    1048
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     932    1465    1749 
   Size:        932     533     284

  Fragments arranged by size: 

                 932     533     284
 
NheI G'CTAG_C

Cuts at:      0    1248    1749 
   Size:       1248     501
 
NspI r_CATG'y

Cuts at:      0     876    1749 
   Size:        876     873
 
PsiI TTA'TAA

Cuts at:      0     769    1749 
   Size:        769     980
 
PstI C_TGCA'G

Cuts at:      0     143     366    1749 
   Size:        143     223    1383

  Fragments arranged by size: 

                1383     223     143
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1028    1058    1749 
   Size:       1028      30     691

  Fragments arranged by size: 

                1028     691      30
 
SexAI A'CCwGG_T

Cuts at:      0      92    1749 
   Size:         92    1657
 
SfcI C'TryA_G

Cuts at:      0     139     362    1749 
   Size:        139     223    1387

  Fragments arranged by size: 

                1387     223     139
 
SspI AAT'ATT

Cuts at:      0     678     741    1749 
   Size:        678      63    1008

  Fragments arranged by size: 

                1008     678      63
 
TatI w'GTAC_w

Cuts at:      0     903    1682    1749 
   Size:        903     779      67

  Fragments arranged by size: 

                 903     779      67
 
XbaI T'CTAG_A

Cuts at:      0     591    1116    1290    1749 
   Size:        591     525     174     459

  Fragments arranged by size: 

                 591     525     459     174
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     397    1749 
   Size:        397    1352
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     741    1749 
   Size:        278     463    1008

  Fragments arranged by size: 

                1008     463     278
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     BanI     BbsI
    BglII     BlpI Bme1580I     BmtI     BpmI   Bpu10I    BseRI    BseYI
 Bsp1286I   BstAPI    BstYI     BtsI     ClaI     EarI  Eco57MI    EcoRI
    Hin4I   HincII  HindIII     HpaI     MmeI     NheI     NspI     PsiI
     PstI     SapI    SexAI     SfcI     SspI     TatI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BmgBI     BmrI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XhoI     XmaI     ZraI