Restriction map for
pF1KB6118
(Linear) (Six-base) MAPSORT of: KIBB6118.seq Check: 9251 from: 1 to: 1638
KIBB6118.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 393 1638
Size: 393 1245
AccI GT'mk_AC
Cuts at: 0 527 1638
Size: 527 1111
AfeI AGC'GCT
Cuts at: 0 299 1638
Size: 299 1339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1043 1638
Size: 1043 595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 1638
Size: 640 32 966
Fragments arranged by size:
966 640 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 348 1358 1638
Size: 180 168 1010 280
Fragments arranged by size:
1010 280 180 168
ApaI G_GGCC'C
Cuts at: 0 989 1227 1638
Size: 989 238 411
Fragments arranged by size:
989 411 238
ApaLI G'TGCA_C
Cuts at: 0 406 493 1638
Size: 406 87 1145
Fragments arranged by size:
1145 406 87
ApoI r'AATT_y
Cuts at: 0 1024 1627 1638
Size: 1024 603 11
Fragments arranged by size:
1024 603 11
AvaI C'yCGr_G
Cuts at: 0 48 981 1606 1638
Size: 48 933 625 32
Fragments arranged by size:
933 625 48 32
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 911 944 1638
Size: 911 33 694
Fragments arranged by size:
911 694 33
BamHI G'GATC_C
Cuts at: 0 212 1638
Size: 212 1426
BanI G'GyrC_C
Cuts at: 0 52 383 693 1013 1156 1638
Size: 52 331 310 320 143 482
Fragments arranged by size:
482 331 320 310 143 52
BanII G_rGCy'C
Cuts at: 0 592 746 989 1227 1638
Size: 592 154 243 238 411
Fragments arranged by size:
592 411 243 238 154
BbeI G_GCGC'C
Cuts at: 0 56 387 1638
Size: 56 331 1251
Fragments arranged by size:
1251 331 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 1451 1638
Size: 1451 187
BbvCI CC'TCA_GC
Cuts at: 0 1125 1470 1638
Size: 1125 345 168
Fragments arranged by size:
1125 345 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 492 526 529 563 1638
Size: 492 34 3 34 1075
Fragments arranged by size:
1075 492 34 34 3
BglI GCCn_nnn'nGGC
Cuts at: 0 381 1011 1429 1638
Size: 381 630 418 209
Fragments arranged by size:
630 418 381 209
BlpI GC'TnA_GC
Cuts at: 0 290 1638
Size: 290 1348
Bme1580I G_kGCm'C
Cuts at: 0 410 497 691 698 989 1016 1161 1169
Size: 410 87 194 7 291 27 145 8
Cuts at: 1169 1227 1638
Size: 58 411
Fragments arranged by size:
411 410 291 194 145 87 58 27
8 7
BmgBI CAC'GTC
Cuts at: 0 1239 1638
Size: 1239 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 1295 1368 1638
Size: 1295 73 270
Fragments arranged by size:
1295 270 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1638
Size: 736 902
Bpu10I CC'TnA_GC
Cuts at: 0 1125 1470 1638
Size: 1125 345 168
Fragments arranged by size:
1125 345 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 1287 1638
Size: 52 1235 351
Fragments arranged by size:
1235 351 52
BsaHI Gr'CG_yC
Cuts at: 0 53 256 384 469 1638
Size: 53 203 128 85 1169
Fragments arranged by size:
1169 203 128 85 53
BsaWI w'CCGG_w
Cuts at: 0 61 581 992 1638
Size: 61 520 411 646
Fragments arranged by size:
646 520 411 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1186 1216 1638
Size: 73 30 1083 30 422
Fragments arranged by size:
1083 422 73 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 340 600 696 1113 1189 1458 1515
Size: 43 297 260 96 417 76 269 57
Cuts at: 1515 1638
Size: 123
Fragments arranged by size:
417 297 269 260 123 96 76 57
43
BseYI C'CCAG_C
Cuts at: 0 173 592 1494 1501 1638
Size: 173 419 902 7 137
Fragments arranged by size:
902 419 173 137 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1280 1638
Size: 340 940 358
Fragments arranged by size:
940 358 340
BsiEI CG_ry'CG
Cuts at: 0 414 1638
Size: 414 1224
BsiHKAI G_wGCw'C
Cuts at: 0 410 497 746 922 1638
Size: 410 87 249 176 716
Fragments arranged by size:
716 410 249 176 87
Bsp1286I G_dGCh'C
Cuts at: 0 410 497 592 691 698 746 922 989
Size: 410 87 95 99 7 48 176 67
Cuts at: 989 1016 1161 1169 1227 1638
Size: 27 145 8 58 411
Fragments arranged by size:
411 410 176 145 99 95 87 67
58 48 27 8 7
BspEI T'CCGG_A
Cuts at: 0 581 992 1638
Size: 581 411 646
Fragments arranged by size:
646 581 411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 393 437 1638
Size: 393 44 1201
Fragments arranged by size:
1201 393 44
BsrBI CCG'CTC
Cuts at: 0 966 1638
Size: 966 672
BsrFI r'CCGG_y
Cuts at: 0 413 1638
Size: 413 1225
BssSI C'ACGA_G
Cuts at: 0 93 745 1481 1638
Size: 93 652 736 157
Fragments arranged by size:
736 652 157 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 1294 1638
Size: 348 946 344
Fragments arranged by size:
946 348 344
BstEII G'GTnAC_C
Cuts at: 0 1410 1638
Size: 1410 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 943 1638
Size: 943 695
BstYI r'GATC_y
Cuts at: 0 212 344 821 1638
Size: 212 132 477 817
Fragments arranged by size:
817 477 212 132
Bsu36I CC'TnA_GG
Cuts at: 0 559 1638
Size: 559 1079
BtgI C'CryG_G
Cuts at: 0 1100 1638
Size: 1100 538
BtsI GCAGTG_nn'
Cuts at: 0 105 364 920 1390 1638
Size: 105 259 556 470 248
Fragments arranged by size:
556 470 259 248 105
ClaI AT'CG_AT
Cuts at: 0 1208 1638
Size: 1208 430
DrdI GACnn_nn'nnGTC
Cuts at: 0 66 1638
Size: 66 1572
EaeI y'GGCC_r
Cuts at: 0 411 1191 1638
Size: 411 780 447
Fragments arranged by size:
780 447 411
EagI C'GGCC_G
Cuts at: 0 411 1638
Size: 411 1227
EarI CTCTTCn'nnn_
Cuts at: 0 110 332 1638
Size: 110 222 1306
Fragments arranged by size:
1306 222 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 285 1638
Size: 285 1353
EcoICRI GAG'CTC
Cuts at: 0 744 1638
Size: 744 894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1638
Size: 736 902
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 322 829 1638
Size: 184 138 507 809
Fragments arranged by size:
809 507 184 138
EcoO109I rG'GnC_Cy
Cuts at: 0 126 985 1108 1223 1638
Size: 126 859 123 115 415
Fragments arranged by size:
859 415 126 123 115
EcoRI G'AATT_C
Cuts at: 0 1627 1638
Size: 1627 11
HaeII r_GCGC'y
Cuts at: 0 56 290 301 387 792 1638
Size: 56 234 11 86 405 846
Fragments arranged by size:
846 405 234 86 56 11
KasI G'GCGC_C
Cuts at: 0 52 383 1638
Size: 52 331 1255
Fragments arranged by size:
1255 331 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 659 1638
Size: 95 564 979
Fragments arranged by size:
979 564 95
MscI TGG'CCA
Cuts at: 0 1193 1638
Size: 1193 445
MslI CAynn'nnrTG
Cuts at: 0 1179 1638
Size: 1179 459
MspA1I CmG'CkG
Cuts at: 0 231 354 573 1129 1638
Size: 231 123 219 556 509
Fragments arranged by size:
556 509 231 219 123
NarI GG'CG_CC
Cuts at: 0 53 384 1638
Size: 53 331 1254
Fragments arranged by size:
1254 331 53
NspI r_CATG'y
Cuts at: 0 1581 1638
Size: 1581 57
PasI CC'CwG_GG
Cuts at: 0 163 463 1162 1591 1638
Size: 163 300 699 429 47
Fragments arranged by size:
699 429 300 163 47
PpuMI rG'GwC_Cy
Cuts at: 0 126 1638
Size: 126 1512
PspOMI G'GGCC_C
Cuts at: 0 985 1223 1638
Size: 985 238 415
Fragments arranged by size:
985 415 238
PstI C_TGCA'G
Cuts at: 0 185 323 353 371 439 1315 1638
Size: 185 138 30 18 68 876 323
Fragments arranged by size:
876 323 185 138 68 30 18
PvuII CAG'CTG
Cuts at: 0 354 573 1129 1638
Size: 354 219 556 509
Fragments arranged by size:
556 509 354 219
SacI G_AGCT'C
Cuts at: 0 746 1638
Size: 746 892
SapI GCTCTTCn'nnn_
Cuts at: 0 332 1638
Size: 332 1306
SbfI CC_TGCA'GG
Cuts at: 0 185 323 1638
Size: 185 138 1315
Fragments arranged by size:
1315 185 138
SfcI C'TryA_G
Cuts at: 0 75 181 319 349 367 435 829 1311
Size: 75 106 138 30 18 68 394 482
Cuts at: 1311 1638
Size: 327
Fragments arranged by size:
482 394 327 138 106 75 68 30
18
SfoI GGC'GCC
Cuts at: 0 54 385 1638
Size: 54 331 1253
Fragments arranged by size:
1253 331 54
SmaI CCC'GGG
Cuts at: 0 983 1608 1638
Size: 983 625 30
Fragments arranged by size:
983 625 30
StuI AGG'CCT
Cuts at: 0 1001 1250 1423 1638
Size: 1001 249 173 215
Fragments arranged by size:
1001 249 215 173
StyI C'CwwG_G
Cuts at: 0 281 1638
Size: 281 1357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1314 1638
Size: 1314 324
TatI w'GTAC_w
Cuts at: 0 873 1638
Size: 873 765
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1186 1218 1360 1392 1638
Size: 73 32 1081 32 142 32 246
Fragments arranged by size:
1081 246 142 73 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 199 1638
Size: 199 1439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 1638
Size: 642 996
XmaI C'CCGG_G
Cuts at: 0 981 1606 1638
Size: 981 625 32
Fragments arranged by size:
981 625 32
Enzymes that do cut:
AarI AccI AfeI AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII KasI MmeI MscI MslI MspA1I
NarI NspI PasI PpuMI PspOMI PstI PvuII SacI
SapI SbfI SfcI SfoI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AscI AseI AsiSI AvrII BciVI BclI BfrBI
BglII BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssHII BstBI BstZ17I BtgZI
CspCI DraI DraIII EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI