Restriction map for
pF1KB6112
(Linear) (Six-base) MAPSORT of: KIBB6112.seq Check: 7150 from: 1 to: 1857
KIBB6112.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 1427 1857
Size: 1427 430
AccI GT'mk_AC
Cuts at: 0 1442 1857
Size: 1442 415
AclI AA'CG_TT
Cuts at: 0 1382 1857
Size: 1382 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 898 964 1384 1643 1750 1857
Size: 631 267 66 420 259 107 107
Fragments arranged by size:
631 420 267 259 107 107 66
AflIII A'CryG_T
Cuts at: 0 42 845 1372 1857
Size: 42 803 527 485
Fragments arranged by size:
803 527 485 42
AhdI GACnn_n'nnGTC
Cuts at: 0 1512 1857
Size: 1512 345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1602 1634 1857
Size: 1602 32 223
Fragments arranged by size:
1602 223 32
AlwNI CAG_nnn'CTG
Cuts at: 0 752 1857
Size: 752 1105
ApaI G_GGCC'C
Cuts at: 0 477 1857
Size: 477 1380
AvaI C'yCGr_G
Cuts at: 0 203 1517 1744 1857
Size: 203 1314 227 113
Fragments arranged by size:
1314 227 203 113
BanI G'GyrC_C
Cuts at: 0 325 867 1677 1857
Size: 325 542 810 180
Fragments arranged by size:
810 542 325 180
BanII G_rGCy'C
Cuts at: 0 303 477 959 1217 1857
Size: 303 174 482 258 640
Fragments arranged by size:
640 482 303 258 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 497 1485 1857
Size: 137 360 988 372
Fragments arranged by size:
988 372 360 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1725 1759 1857
Size: 1725 34 98
Fragments arranged by size:
1725 98 34
BclI T'GATC_A
Cuts at: 0 641 1857
Size: 641 1216
BglI GCCn_nnn'nGGC
Cuts at: 0 1055 1857
Size: 1055 802
BglII A'GATC_T
Cuts at: 0 1792 1857
Size: 1792 65
BlpI GC'TnA_GC
Cuts at: 0 1293 1857
Size: 1293 564
Bme1580I G_kGCm'C
Cuts at: 0 437 477 1682 1857
Size: 437 40 1205 175
Fragments arranged by size:
1205 437 175 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 471 1722 1788 1857
Size: 471 1251 66 69
Fragments arranged by size:
1251 471 69 66
BmtI G_CTAG'C
Cuts at: 0 1568 1857
Size: 1568 289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 1220 1653 1676 1857
Size: 290 930 433 23 181
Fragments arranged by size:
930 433 290 181 23
Bpu10I CC'TnA_GC
Cuts at: 0 406 794 1252 1857
Size: 406 388 458 605
Fragments arranged by size:
605 458 406 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 834 1801 1857
Size: 175 659 967 56
Fragments arranged by size:
967 659 175 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 989 1196 1857
Size: 989 207 661
Fragments arranged by size:
989 661 207
BsaBI GATnn'nnATC
Cuts at: 0 266 562 1857
Size: 266 296 1295
Fragments arranged by size:
1295 296 266
BsaHI Gr'CG_yC
Cuts at: 0 494 1424 1857
Size: 494 930 433
Fragments arranged by size:
930 494 433
BsaWI w'CCGG_w
Cuts at: 0 86 1857
Size: 86 1771
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 649 679 1199 1229 1674 1704 1857
Size: 649 30 520 30 445 30 153
Fragments arranged by size:
649 520 445 153 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 329 1857
Size: 293 36 1528
Fragments arranged by size:
1528 293 36
BseYI C'CCAG_C
Cuts at: 0 1415 1857
Size: 1415 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1637 1857
Size: 447 1190 220
Fragments arranged by size:
1190 447 220
BsiHKAI G_wGCw'C
Cuts at: 0 303 959 1857
Size: 303 656 898
Fragments arranged by size:
898 656 303
BsmI GAATG_Cn'
Cuts at: 0 272 1256 1617 1717 1846 1857
Size: 272 984 361 100 129 11
Fragments arranged by size:
984 361 272 129 100 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1381 1857
Size: 1381 476
Bsp1286I G_dGCh'C
Cuts at: 0 303 437 477 959 1217 1682 1857
Size: 303 134 40 482 258 465 175
Fragments arranged by size:
482 465 303 258 175 134 40
BspHI T'CATG_A
Cuts at: 0 644 1850 1857
Size: 644 1206 7
Fragments arranged by size:
1206 644 7
BsrBI CCG'CTC
Cuts at: 0 918 1857
Size: 918 939
BsrDI GCAATG_nn'
Cuts at: 0 185 1465 1550 1857
Size: 185 1280 85 307
Fragments arranged by size:
1280 307 185 85
BsrGI T'GTAC_A
Cuts at: 0 830 1857
Size: 830 1027
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 1857
Size: 1170 687
BstYI r'GATC_y
Cuts at: 0 881 1628 1792 1832 1857
Size: 881 747 164 40 25
Fragments arranged by size:
881 747 164 40 25
Bsu36I CC'TnA_GG
Cuts at: 0 769 1857
Size: 769 1088
BtsI GCAGTG_nn'
Cuts at: 0 435 1857
Size: 435 1422
DrdI GACnn_nn'nnGTC
Cuts at: 0 239 1857
Size: 239 1618
EaeI y'GGCC_r
Cuts at: 0 1056 1857
Size: 1056 801
EarI CTCTTCn'nnn_
Cuts at: 0 117 582 776 947 1144 1619 1857
Size: 117 465 194 171 197 475 238
Fragments arranged by size:
475 465 238 197 194 171 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1857
Size: 19 1838
EcoICRI GAG'CTC
Cuts at: 0 301 957 1857
Size: 301 656 900
Fragments arranged by size:
900 656 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 631 898 964 1220 1384 1643 1653
Size: 290 341 267 66 256 164 259 10
Cuts at: 1653 1676 1750 1857
Size: 23 74 107
Fragments arranged by size:
341 290 267 259 256 164 107 74
66 23 10
EcoO109I rG'GnC_Cy
Cuts at: 0 79 341 1238 1857
Size: 79 262 897 619
Fragments arranged by size:
897 619 262 79
EcoRV GAT'ATC
Cuts at: 0 560 1857
Size: 560 1297
HaeII r_GCGC'y
Cuts at: 0 1019 1454 1857
Size: 1019 435 403
Fragments arranged by size:
1019 435 403
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 328 360 525 557 787 819
Size: 1 32 295 32 165 32 230 32
Cuts at: 819 832 864 958 990 1199 1231 1498 1530
Size: 13 32 94 32 209 32 267 32
Cuts at: 1530 1857
Size: 327
Fragments arranged by size:
327 295 267 230 209 165 94 32
32 32 32 32 32 32 32 13
1
HincII GTy'rAC
Cuts at: 0 525 1857
Size: 525 1332
HindIII A'AGCT_T
Cuts at: 0 1330 1857
Size: 1330 527
MfeI C'AATT_G
Cuts at: 0 1076 1857
Size: 1076 781
MluI A'CGCG_T
Cuts at: 0 42 1372 1857
Size: 42 1330 485
Fragments arranged by size:
1330 485 42
MscI TGG'CCA
Cuts at: 0 1058 1857
Size: 1058 799
MslI CAynn'nnrTG
Cuts at: 0 35 1470 1857
Size: 35 1435 387
Fragments arranged by size:
1435 387 35
NheI G'CTAG_C
Cuts at: 0 1564 1857
Size: 1564 293
NruI TCG'CGA
Cuts at: 0 1025 1857
Size: 1025 832
NspI r_CATG'y
Cuts at: 0 849 1857
Size: 849 1008
PciI A'CATG_T
Cuts at: 0 845 1857
Size: 845 1012
PfoI T'CCnGG_A
Cuts at: 0 1217 1857
Size: 1217 640
PpuMI rG'GwC_Cy
Cuts at: 0 79 341 1857
Size: 79 262 1516
Fragments arranged by size:
1516 262 79
PspOMI G'GGCC_C
Cuts at: 0 473 1857
Size: 473 1384
PspXI vC'TCGA_Gb
Cuts at: 0 1744 1857
Size: 1744 113
SacI G_AGCT'C
Cuts at: 0 303 959 1857
Size: 303 656 898
Fragments arranged by size:
898 656 303
SanDI GG'GwC_CC
Cuts at: 0 79 1857
Size: 79 1778
SapI GCTCTTCn'nnn_
Cuts at: 0 947 1857
Size: 947 910
ScaI AGT'ACT
Cuts at: 0 501 1180 1857
Size: 501 679 677
Fragments arranged by size:
679 677 501
SmaI CCC'GGG
Cuts at: 0 1519 1857
Size: 1519 338
SmlI C'TyrA_G
Cuts at: 0 154 813 1744 1816 1857
Size: 154 659 931 72 41
Fragments arranged by size:
931 659 154 72 41
StuI AGG'CCT
Cuts at: 0 692 1804 1857
Size: 692 1112 53
Fragments arranged by size:
1112 692 53
StyI C'CwwG_G
Cuts at: 0 1823 1857
Size: 1823 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 885 1220 1671 1857
Size: 131 754 335 451 186
Fragments arranged by size:
754 451 335 186 131
TatI w'GTAC_w
Cuts at: 0 499 830 1178 1857
Size: 499 331 348 679
Fragments arranged by size:
679 499 348 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 84 116 1674 1706 1857
Size: 84 32 1558 32 151
Fragments arranged by size:
1558 151 84 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 733 1857
Size: 733 1124
XhoI C'TCGA_G
Cuts at: 0 1744 1857
Size: 1744 113
XmaI C'CCGG_G
Cuts at: 0 1517 1857
Size: 1517 340
XmnI GAAnn'nnTTC
Cuts at: 0 355 1031 1857
Size: 355 676 826
Fragments arranged by size:
826 676 355
ZraI GAC'GTC
Cuts at: 0 1425 1857
Size: 1425 432
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AhdI AloI AlwNI
ApaI AvaI BanI BanII BbsI BcgI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstAPI BstYI Bsu36I BtsI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII
MfeI MluI MscI MslI NheI NruI NspI PciI
PfoI PpuMI PspOMI PspXI SacI SanDI SapI ScaI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AleI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BfrBI BmgBI BplI BsaAI BsiEI BsiWI
BspEI BspMI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EagI
EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI
KpnI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SacII
SalI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI