Restriction map for
pF1KB6107
(Linear) (Six-base) MAPSORT of: KIBB6107.seq Check: 6232 from: 1 to: 1740
KIBB6107.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1337 1740
Size: 1337 403
Acc65I G'GTAC_C
Cuts at: 0 617 1740
Size: 617 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1505 1740
Size: 499 1006 235
Fragments arranged by size:
1006 499 235
AgeI A'CCGG_T
Cuts at: 0 641 707 1740
Size: 641 66 1033
Fragments arranged by size:
1033 641 66
AhdI GACnn_n'nnGTC
Cuts at: 0 108 1740
Size: 108 1632
AleI CACnn'nnGTG
Cuts at: 0 913 1740
Size: 913 827
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1075 1107 1740
Size: 1075 32 633
Fragments arranged by size:
1075 633 32
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1710 1740
Size: 414 1296 30
Fragments arranged by size:
1296 414 30
ApaI G_GGCC'C
Cuts at: 0 75 608 748 1740
Size: 75 533 140 992
Fragments arranged by size:
992 533 140 75
ApoI r'AATT_y
Cuts at: 0 1144 1286 1740
Size: 1144 142 454
Fragments arranged by size:
1144 454 142
AvaI C'yCGr_G
Cuts at: 0 29 169 538 547 1528 1740
Size: 29 140 369 9 981 212
Fragments arranged by size:
981 369 212 140 29 9
AvrII C'CTAG_G
Cuts at: 0 306 1740
Size: 306 1434
BamHI G'GATC_C
Cuts at: 0 18 1740
Size: 18 1722
BanI G'GyrC_C
Cuts at: 0 43 487 617 1740
Size: 43 444 130 1123
Fragments arranged by size:
1123 444 130 43
BanII G_rGCy'C
Cuts at: 0 15 75 290 548 608 692 748 884
Size: 15 60 215 258 60 84 56 136
Cuts at: 884 1740
Size: 856
Fragments arranged by size:
856 258 215 136 84 60 60 56
15
BbeI G_GCGC'C
Cuts at: 0 491 1740
Size: 491 1249
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 1740
Size: 319 1421
BbvCI CC'TCA_GC
Cuts at: 0 1230 1740
Size: 1230 510
BlpI GC'TnA_GC
Cuts at: 0 1475 1740
Size: 1475 265
Bme1580I G_kGCm'C
Cuts at: 0 75 608 748 1740
Size: 75 533 140 992
Fragments arranged by size:
992 533 140 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 249 669 885 907 1208 1740
Size: 249 420 216 22 301 532
Fragments arranged by size:
532 420 301 249 216 22
BmtI G_CTAG'C
Cuts at: 0 1262 1740
Size: 1262 478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 1740
Size: 573 1167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1380 1740
Size: 1257 123 360
Fragments arranged by size:
1257 360 123
Bpu10I CC'TnA_GC
Cuts at: 0 1230 1311 1740
Size: 1230 81 429
Fragments arranged by size:
1230 429 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1588 1740
Size: 1588 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 698 1740
Size: 698 1042
BsaBI GATnn'nnATC
Cuts at: 0 340 1740
Size: 340 1400
BsaHI Gr'CG_yC
Cuts at: 0 488 1740
Size: 488 1252
BsaWI w'CCGG_w
Cuts at: 0 641 707 1740
Size: 641 66 1033
Fragments arranged by size:
1033 641 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 277 307 535 565 920 950
Size: 115 30 132 30 228 30 355 30
Cuts at: 950 1075 1105 1740
Size: 125 30 635
Fragments arranged by size:
635 355 228 132 125 115 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 556 862 928 1083 1740
Size: 298 258 306 66 155 657
Fragments arranged by size:
657 306 298 258 155 66
BseYI C'CCAG_C
Cuts at: 0 1636 1740
Size: 1636 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 666 1529 1652 1740
Size: 654 12 863 123 88
Fragments arranged by size:
863 654 123 88 12
BsiHKAI G_wGCw'C
Cuts at: 0 15 548 884 1243 1740
Size: 15 533 336 359 497
Fragments arranged by size:
533 497 359 336 15
Bsp1286I G_dGCh'C
Cuts at: 0 15 75 290 548 608 692 748 884
Size: 15 60 215 258 60 84 56 136
Cuts at: 884 1243 1740
Size: 359 497
Fragments arranged by size:
497 359 258 215 136 84 60 60
56 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 629 1740
Size: 629 1111
BsrBI CCG'CTC
Cuts at: 0 1533 1740
Size: 1533 207
BsrDI GCAATG_nn'
Cuts at: 0 589 640 1740
Size: 589 51 1100
Fragments arranged by size:
1100 589 51
BsrFI r'CCGG_y
Cuts at: 0 143 268 641 707 965 1740
Size: 143 125 373 66 258 775
Fragments arranged by size:
775 373 258 143 125 66
BssHII G'CGCG_C
Cuts at: 0 473 1740
Size: 473 1267
BssSI C'ACGA_G
Cuts at: 0 211 661 1740
Size: 211 450 1079
Fragments arranged by size:
1079 450 211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 302 677 1740
Size: 302 375 1063
Fragments arranged by size:
1063 375 302
BstBI TT'CG_AA
Cuts at: 0 221 1740
Size: 221 1519
BstEII G'GTnAC_C
Cuts at: 0 364 1740
Size: 364 1376
BstYI r'GATC_y
Cuts at: 0 18 335 1740
Size: 18 317 1405
Fragments arranged by size:
1405 317 18
Bsu36I CC'TnA_GG
Cuts at: 0 1691 1740
Size: 1691 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 477 550 789 1740
Size: 477 73 239 951
Fragments arranged by size:
951 477 239 73
BtsI GCAGTG_nn'
Cuts at: 0 382 405 658 1512 1740
Size: 382 23 253 854 228
Fragments arranged by size:
854 382 253 228 23
DraI TTT'AAA
Cuts at: 0 1298 1740
Size: 1298 442
DraIII CAC_nnn'GTG
Cuts at: 0 677 1740
Size: 677 1063
EaeI y'GGCC_r
Cuts at: 0 1534 1740
Size: 1534 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1000 1740
Size: 1000 740
EcoICRI GAG'CTC
Cuts at: 0 13 546 882 1740
Size: 13 533 336 858
Fragments arranged by size:
858 533 336 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1257 1380 1505 1740
Size: 499 758 123 125 235
Fragments arranged by size:
758 499 235 125 123
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 250 1061 1740
Size: 79 171 811 679
Fragments arranged by size:
811 679 171 79
EcoO109I rG'GnC_Cy
Cuts at: 0 71 72 243 744 1740
Size: 71 1 171 501 996
Fragments arranged by size:
996 501 171 71 1
EcoRI G'AATT_C
Cuts at: 0 1144 1740
Size: 1144 596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 852 1023 1740
Size: 111 741 171 717
Fragments arranged by size:
741 717 171 111
FspI TGC'GCA
Cuts at: 0 1018 1740
Size: 1018 722
HaeII r_GCGC'y
Cuts at: 0 491 1091 1740
Size: 491 600 649
Fragments arranged by size:
649 600 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 428 460 920 952 1213 1245
Size: 94 32 302 32 460 32 261 32
Cuts at: 1245 1324 1356 1573 1605 1740
Size: 79 32 217 32 135
Fragments arranged by size:
460 302 261 217 135 94 79 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 867 1338 1740
Size: 867 471 402
Fragments arranged by size:
867 471 402
KasI G'GCGC_C
Cuts at: 0 487 1740
Size: 487 1253
KpnI G_GTAC'C
Cuts at: 0 621 1740
Size: 621 1119
MslI CAynn'nnrTG
Cuts at: 0 269 913 1740
Size: 269 644 827
Fragments arranged by size:
827 644 269
MspA1I CmG'CkG
Cuts at: 0 327 1474 1636 1740
Size: 327 1147 162 104
Fragments arranged by size:
1147 327 162 104
NarI GG'CG_CC
Cuts at: 0 488 1740
Size: 488 1252
NheI G'CTAG_C
Cuts at: 0 1258 1740
Size: 1258 482
NspI r_CATG'y
Cuts at: 0 268 1449 1740
Size: 268 1181 291
Fragments arranged by size:
1181 291 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 239 1254 1671 1740
Size: 239 1015 417 69
Fragments arranged by size:
1015 417 239 69
PfoI T'CCnGG_A
Cuts at: 0 276 1740
Size: 276 1464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1075 1107 1740
Size: 1075 32 633
Fragments arranged by size:
1075 633 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 1740
Size: 243 1497
PsiI TTA'TAA
Cuts at: 0 959 1740
Size: 959 781
PspOMI G'GGCC_C
Cuts at: 0 71 604 744 1740
Size: 71 533 140 996
Fragments arranged by size:
996 533 140 71
PspXI vC'TCGA_Gb
Cuts at: 0 547 1740
Size: 547 1193
PstI C_TGCA'G
Cuts at: 0 509 890 1740
Size: 509 381 850
Fragments arranged by size:
850 509 381
PvuII CAG'CTG
Cuts at: 0 1474 1636 1740
Size: 1474 162 104
Fragments arranged by size:
1474 162 104
SacI G_AGCT'C
Cuts at: 0 15 548 884 1740
Size: 15 533 336 856
Fragments arranged by size:
856 533 336 15
SalI G'TCGA_C
Cuts at: 0 1336 1740
Size: 1336 404
ScaI AGT'ACT
Cuts at: 0 835 1740
Size: 835 905
SexAI A'CCwGG_T
Cuts at: 0 1664 1709 1740
Size: 1664 45 31
Fragments arranged by size:
1664 45 31
SfcI C'TryA_G
Cuts at: 0 505 886 1740
Size: 505 381 854
Fragments arranged by size:
854 505 381
SfoI GGC'GCC
Cuts at: 0 489 1740
Size: 489 1251
SmlI C'TyrA_G
Cuts at: 0 547 1603 1740
Size: 547 1056 137
Fragments arranged by size:
1056 547 137
SphI G_CATG'C
Cuts at: 0 268 1740
Size: 268 1472
StuI AGG'CCT
Cuts at: 0 667 1740
Size: 667 1073
StyI C'CwwG_G
Cuts at: 0 306 424 1436 1740
Size: 306 118 1012 304
Fragments arranged by size:
1012 306 304 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 688 768 1047 1740
Size: 688 80 279 693
Fragments arranged by size:
693 688 279 80
TatI w'GTAC_w
Cuts at: 0 161 752 833 1740
Size: 161 591 81 907
Fragments arranged by size:
907 591 161 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 535 567 1740
Size: 277 32 226 32 1173
Fragments arranged by size:
1173 277 226 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1251 1404 1740
Size: 1251 153 336
Fragments arranged by size:
1251 336 153
XhoI C'TCGA_G
Cuts at: 0 547 1740
Size: 547 1193
XmnI GAAnn'nnTTC
Cuts at: 0 94 1740
Size: 94 1646
Enzymes that do cut:
AccI Acc65I AcuI AgeI AhdI AleI AloI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI DraI DraIII EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MslI MspA1I NarI NheI NspI PflMI PfoI
PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI
SalI ScaI SexAI SfcI SfoI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AlfI ApaLI
AscI AseI AsiSI BaeI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BsaAI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BstXI BstZ17I BtgI ClaI CspCI
DrdI EagI EarI EcoRV FseI FspAI HindIII HpaI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsrI PvuI RsrII SacII SanDI SapI SbfI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XmaI ZraI