Restriction map for
pF1KB6101
(Linear) (Six-base) MAPSORT of: KIBB6101.seq Check: 7115 from: 1 to: 1731
KIBB6101.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 854 872 1731
Size: 854 18 859
Fragments arranged by size:
859 854 18
AccI GT'mk_AC
Cuts at: 0 831 1046 1731
Size: 831 215 685
Fragments arranged by size:
831 685 215
AclI AA'CG_TT
Cuts at: 0 87 1731
Size: 87 1644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1731
Size: 1333 398
AflIII A'CryG_T
Cuts at: 0 1172 1731
Size: 1172 559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 494 1731
Size: 494 1237
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 1731
Size: 1117 614
ApaI G_GGCC'C
Cuts at: 0 1400 1731
Size: 1400 331
AvaI C'yCGr_G
Cuts at: 0 855 1667 1731
Size: 855 812 64
Fragments arranged by size:
855 812 64
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1161 1194 1731
Size: 1161 33 537
Fragments arranged by size:
1161 537 33
BamHI G'GATC_C
Cuts at: 0 1260 1567 1731
Size: 1260 307 164
Fragments arranged by size:
1260 307 164
BanI G'GyrC_C
Cuts at: 0 16 1684 1731
Size: 16 1668 47
Fragments arranged by size:
1668 47 16
BanII G_rGCy'C
Cuts at: 0 11 805 1390 1400 1731
Size: 11 794 585 10 331
Fragments arranged by size:
794 585 331 11 10
BbeI G_GCGC'C
Cuts at: 0 20 1731
Size: 20 1711
BbvCI CC'TCA_GC
Cuts at: 0 534 1731
Size: 534 1197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 351 385 1271 1305 1424 1458 1469 1503
Size: 351 34 886 34 119 34 11 34
Cuts at: 1503 1731
Size: 228
Fragments arranged by size:
886 351 228 119 34 34 34 34
11
BciVI GTATCCnnnnn_n'
Cuts at: 0 1189 1731
Size: 1189 542
BglI GCCn_nnn'nGGC
Cuts at: 0 481 1731
Size: 481 1250
BglII A'GATC_T
Cuts at: 0 1310 1367 1731
Size: 1310 57 364
Fragments arranged by size:
1310 364 57
BlpI GC'TnA_GC
Cuts at: 0 464 1731
Size: 464 1267
Bme1580I G_kGCm'C
Cuts at: 0 1400 1731
Size: 1400 331
BmgBI CAC'GTC
Cuts at: 0 423 1731
Size: 423 1308
BmrI ACTGGGnnnn_n'
Cuts at: 0 1204 1403 1731
Size: 1204 199 328
Fragments arranged by size:
1204 328 199
BmtI G_CTAG'C
Cuts at: 0 742 1731
Size: 742 989
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 1731
Size: 475 1256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 295 773 1335 1731
Size: 184 111 478 562 396
Fragments arranged by size:
562 478 396 184 111
Bpu10I CC'TnA_GC
Cuts at: 0 534 1731
Size: 534 1197
BsaBI GATnn'nnATC
Cuts at: 0 346 1731
Size: 346 1385
BsaHI Gr'CG_yC
Cuts at: 0 17 407 851 869 1484 1731
Size: 17 390 444 18 615 247
Fragments arranged by size:
615 444 390 247 18 17
BsaWI w'CCGG_w
Cuts at: 0 745 1731
Size: 745 986
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 787 817 1402 1432 1664 1694
Size: 90 30 667 30 585 30 232 30
Cuts at: 1694 1731
Size: 37
Fragments arranged by size:
667 585 232 90 37 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1685 1731
Size: 1685 46
BseYI C'CCAG_C
Cuts at: 0 809 1731
Size: 809 922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1209 1473 1731
Size: 448 761 264 258
Fragments arranged by size:
761 448 264 258
BsiEI CG_ry'CG
Cuts at: 0 293 378 1731
Size: 293 85 1353
Fragments arranged by size:
1353 293 85
BsiHKAI G_wGCw'C
Cuts at: 0 11 606 1731
Size: 11 595 1125
Fragments arranged by size:
1125 595 11
BsmI GAATG_Cn'
Cuts at: 0 445 1731
Size: 445 1286
Bsp1286I G_dGCh'C
Cuts at: 0 11 606 805 1390 1400 1731
Size: 11 595 199 585 10 331
Fragments arranged by size:
595 585 331 199 11 10
BspEI T'CCGG_A
Cuts at: 0 745 1731
Size: 745 986
BsrFI r'CCGG_y
Cuts at: 0 288 538 1731
Size: 288 250 1193
Fragments arranged by size:
1193 288 250
BssSI C'ACGA_G
Cuts at: 0 814 1731
Size: 814 917
BstEII G'GTnAC_C
Cuts at: 0 955 1731
Size: 955 776
BstYI r'GATC_y
Cuts at: 0 748 1260 1310 1367 1567 1719 1731
Size: 748 512 50 57 200 152 12
Fragments arranged by size:
748 512 200 152 57 50 12
BstZ17I GTA'TAC
Cuts at: 0 832 1731
Size: 832 899
Bsu36I CC'TnA_GG
Cuts at: 0 972 1731
Size: 972 759
BtgI C'CryG_G
Cuts at: 0 503 578 1731
Size: 503 75 1153
Fragments arranged by size:
1153 503 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 1731
Size: 116 1615
DrdI GACnn_nn'nnGTC
Cuts at: 0 1210 1582 1731
Size: 1210 372 149
Fragments arranged by size:
1210 372 149
EaeI y'GGCC_r
Cuts at: 0 290 375 500 962 1559 1731
Size: 290 85 125 462 597 172
Fragments arranged by size:
597 462 290 172 125 85
EagI C'GGCC_G
Cuts at: 0 290 375 1731
Size: 290 85 1356
Fragments arranged by size:
1356 290 85
EarI CTCTTCn'nnn_
Cuts at: 0 27 45 587 1731
Size: 27 18 542 1144
Fragments arranged by size:
1144 542 27 18
EcoICRI GAG'CTC
Cuts at: 0 9 1731
Size: 9 1722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 295 773 1333 1335 1731
Size: 184 111 478 560 2 396
Fragments arranged by size:
560 478 396 184 111 2
EcoO109I rG'GnC_Cy
Cuts at: 0 318 988 1731
Size: 318 670 743
Fragments arranged by size:
743 670 318
HaeII r_GCGC'y
Cuts at: 0 20 1731
Size: 20 1711
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 646 678 859 891 1596 1628
Size: 379 32 235 32 181 32 705 32
Cuts at: 1628 1731
Size: 103
Fragments arranged by size:
705 379 235 181 103 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1245 1731
Size: 1245 486
KasI G'GCGC_C
Cuts at: 0 16 1731
Size: 16 1715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1445 1721 1731
Size: 287 1158 276 10
Fragments arranged by size:
1158 287 276 10
MscI TGG'CCA
Cuts at: 0 964 1561 1731
Size: 964 597 170
Fragments arranged by size:
964 597 170
MslI CAynn'nnrTG
Cuts at: 0 1171 1731
Size: 1171 560
NaeI GCC'GGC
Cuts at: 0 540 1731
Size: 540 1191
NarI GG'CG_CC
Cuts at: 0 17 1731
Size: 17 1714
NdeI CA'TA_TG
Cuts at: 0 921 1731
Size: 921 810
NgoMIV G'CCGG_C
Cuts at: 0 538 1731
Size: 538 1193
NheI G'CTAG_C
Cuts at: 0 738 1731
Size: 738 993
NspI r_CATG'y
Cuts at: 0 1176 1731
Size: 1176 555
PciI A'CATG_T
Cuts at: 0 1172 1731
Size: 1172 559
PflMI CCAn_nnn'nTGG
Cuts at: 0 1256 1382 1731
Size: 1256 126 349
Fragments arranged by size:
1256 349 126
PfoI T'CCnGG_A
Cuts at: 0 200 1731
Size: 200 1531
PspOMI G'GGCC_C
Cuts at: 0 1396 1731
Size: 1396 335
PstI C_TGCA'G
Cuts at: 0 48 326 1731
Size: 48 278 1405
Fragments arranged by size:
1405 278 48
SacI G_AGCT'C
Cuts at: 0 11 1731
Size: 11 1720
SbfI CC_TGCA'GG
Cuts at: 0 48 1731
Size: 48 1683
ScaI AGT'ACT
Cuts at: 0 1555 1731
Size: 1555 176
SfcI C'TryA_G
Cuts at: 0 44 322 1161 1524 1731
Size: 44 278 839 363 207
Fragments arranged by size:
839 363 278 207 44
SfoI GGC'GCC
Cuts at: 0 18 1731
Size: 18 1713
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 823 1507 1672 1731
Size: 19 804 684 165 59
Fragments arranged by size:
804 684 165 59 19
TatI w'GTAC_w
Cuts at: 0 1553 1731
Size: 1553 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 1664 1696 1731
Size: 90 32 1542 32 35
Fragments arranged by size:
1542 90 35 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1428 1731
Size: 1428 303
ZraI GAC'GTC
Cuts at: 0 852 870 1731
Size: 852 18 861
Fragments arranged by size:
861 852 18
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AlfI AlwNI ApaI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrFI
BssSI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KasI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PspOMI PstI
SacI SbfI ScaI SfcI SfoI TaqII TatI TstI
XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BbsI BclI
BfrBI BpuEI BsaI BsaAI BsiWI BsmBI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstXI BtsI
ClaI CspCI DraI DraIII EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MspA1I NcoI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI
XmaI XmnI