Restriction map for
pF1KB6013
(Linear) (Six-base) MAPSORT of: KIBB6013.seq Check: 1144 from: 1 to: 1728
KIBB6013.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 190 865 1728
Size: 33 157 675 863
Fragments arranged by size:
863 675 157 33
AatII G_ACGT'C
Cuts at: 0 1703 1728
Size: 1703 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1728
Size: 1006 722
AfeI AGC'GCT
Cuts at: 0 53 1330 1728
Size: 53 1277 398
Fragments arranged by size:
1277 398 53
AflIII A'CryG_T
Cuts at: 0 328 1728
Size: 328 1400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1055 1563 1630 1728
Size: 1055 508 67 98
Fragments arranged by size:
1055 508 98 67
AlwNI CAG_nnn'CTG
Cuts at: 0 1373 1728
Size: 1373 355
ApaI G_GGCC'C
Cuts at: 0 9 304 1181 1728
Size: 9 295 877 547
Fragments arranged by size:
877 547 295 9
ApaLI G'TGCA_C
Cuts at: 0 649 1728
Size: 649 1079
AvaI C'yCGr_G
Cuts at: 0 76 1431 1728
Size: 76 1355 297
Fragments arranged by size:
1355 297 76
BanI G'GyrC_C
Cuts at: 0 81 1728
Size: 81 1647
BanII G_rGCy'C
Cuts at: 0 9 304 361 425 1181 1728
Size: 9 295 57 64 756 547
Fragments arranged by size:
756 547 295 64 57 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1696 1728
Size: 1696 32
BglI GCCn_nnn'nGGC
Cuts at: 0 308 956 1223 1728
Size: 308 648 267 505
Fragments arranged by size:
648 505 308 267
Bme1580I G_kGCm'C
Cuts at: 0 9 86 304 653 1181 1309 1653 1728
Size: 9 77 218 349 528 128 344 75
Fragments arranged by size:
528 349 344 218 128 77 75 9
BmgBI CAC'GTC
Cuts at: 0 285 1728
Size: 285 1443
BmrI ACTGGGnnnn_n'
Cuts at: 0 298 1545 1728
Size: 298 1247 183
Fragments arranged by size:
1247 298 183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 507 1446 1728
Size: 507 939 282
Fragments arranged by size:
939 507 282
Bpu10I CC'TnA_GC
Cuts at: 0 608 1728
Size: 608 1120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 1690 1728
Size: 1471 219 38
Fragments arranged by size:
1471 219 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 211 562 595 1099 1728
Size: 35 176 351 33 504 629
Fragments arranged by size:
629 504 351 176 35 33
BsaAI yAC'GTr
Cuts at: 0 1530 1728
Size: 1530 198
BsaHI Gr'CG_yC
Cuts at: 0 1700 1728
Size: 1700 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 721 751 1728
Size: 721 30 977
Fragments arranged by size:
977 721 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1312 1435 1728
Size: 1312 123 293
Fragments arranged by size:
1312 293 123
BseYI C'CCAG_C
Cuts at: 0 35 424 809 830 844 877 1202 1728
Size: 35 389 385 21 14 33 325 526
Fragments arranged by size:
526 389 385 325 35 33 21 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1176 1728
Size: 843 333 552
Fragments arranged by size:
843 552 333
BsiEI CG_ry'CG
Cuts at: 0 977 1728
Size: 977 751
BsiHKAI G_wGCw'C
Cuts at: 0 181 653 1728
Size: 181 472 1075
Fragments arranged by size:
1075 472 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 193 1728
Size: 193 1535
Bsp1286I G_dGCh'C
Cuts at: 0 9 86 181 304 361 425 653 1181
Size: 9 77 95 123 57 64 228 528
Cuts at: 1181 1309 1653 1728
Size: 128 344 75
Fragments arranged by size:
528 344 228 128 123 95 77 75
64 57 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 190 442 529 865 1728
Size: 33 157 252 87 336 863
Fragments arranged by size:
863 336 252 157 87 33
BsrBI CCG'CTC
Cuts at: 0 1139 1578 1728
Size: 1139 439 150
Fragments arranged by size:
1139 439 150
BsrDI GCAATG_nn'
Cuts at: 0 803 1269 1728
Size: 803 466 459
Fragments arranged by size:
803 466 459
BsrFI r'CCGG_y
Cuts at: 0 20 127 704 1728
Size: 20 107 577 1024
Fragments arranged by size:
1024 577 107 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1648 1728
Size: 1648 80
BstEII G'GTnAC_C
Cuts at: 0 660 795 1728
Size: 660 135 933
Fragments arranged by size:
933 660 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 781 1728
Size: 781 947
BstYI r'GATC_y
Cuts at: 0 848 1728
Size: 848 880
Bsu36I CC'TnA_GG
Cuts at: 0 886 1728
Size: 886 842
BtgI C'CryG_G
Cuts at: 0 665 1728
Size: 665 1063
BtsI GCAGTG_nn'
Cuts at: 0 1077 1728
Size: 1077 651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 228 263 1728
Size: 228 35 1465
Fragments arranged by size:
1465 228 35
EaeI y'GGCC_r
Cuts at: 0 381 509 974 1074 1148 1610 1728
Size: 381 128 465 100 74 462 118
Fragments arranged by size:
465 462 381 128 118 100 74
EagI C'GGCC_G
Cuts at: 0 974 1728
Size: 974 754
EarI CTCTTCn'nnn_
Cuts at: 0 1010 1728
Size: 1010 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1728
Size: 1006 722
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1314 1498 1728
Size: 1314 184 230
Fragments arranged by size:
1314 230 184
EcoO109I rG'GnC_Cy
Cuts at: 0 6 300 882 1177 1178 1461 1728
Size: 6 294 582 295 1 283 267
Fragments arranged by size:
582 295 294 283 267 6 1
FseI GG_CCGG'CC
Cuts at: 0 708 1728
Size: 708 1020
HaeII r_GCGC'y
Cuts at: 0 55 103 1332 1728
Size: 55 48 1229 396
Fragments arranged by size:
1229 396 55 48
HindIII A'AGCT_T
Cuts at: 0 1666 1728
Size: 1666 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1728
Size: 719 1009
MscI TGG'CCA
Cuts at: 0 383 1612 1728
Size: 383 1229 116
Fragments arranged by size:
1229 383 116
MslI CAynn'nnrTG
Cuts at: 0 283 779 1037 1585 1612 1728
Size: 283 496 258 548 27 116
Fragments arranged by size:
548 496 283 258 116 27
MspA1I CmG'CkG
Cuts at: 0 410 467 813 834 903 1028 1146 1728
Size: 410 57 346 21 69 125 118 582
Fragments arranged by size:
582 410 346 125 118 69 57 21
NaeI GCC'GGC
Cuts at: 0 129 706 1728
Size: 129 577 1022
Fragments arranged by size:
1022 577 129
NcoI C'CATG_G
Cuts at: 0 665 1728
Size: 665 1063
NgoMIV G'CCGG_C
Cuts at: 0 127 704 1728
Size: 127 577 1024
Fragments arranged by size:
1024 577 127
NspI r_CATG'y
Cuts at: 0 315 332 1125 1544 1728
Size: 315 17 793 419 184
Fragments arranged by size:
793 419 315 184 17
PasI CC'CwG_GG
Cuts at: 0 1316 1728
Size: 1316 412
PciI A'CATG_T
Cuts at: 0 328 1728
Size: 328 1400
PflMI CCAn_nnn'nTGG
Cuts at: 0 161 1728
Size: 161 1567
PmlI CAC'GTG
Cuts at: 0 1530 1728
Size: 1530 198
PpuMI rG'GwC_Cy
Cuts at: 0 882 1461 1728
Size: 882 579 267
Fragments arranged by size:
882 579 267
PspOMI G'GGCC_C
Cuts at: 0 5 300 1177 1728
Size: 5 295 877 551
Fragments arranged by size:
877 551 295 5
PstI C_TGCA'G
Cuts at: 0 187 353 839 933 1175 1728
Size: 187 166 486 94 242 553
Fragments arranged by size:
553 486 242 187 166 94
PvuII CAG'CTG
Cuts at: 0 813 834 1728
Size: 813 21 894
Fragments arranged by size:
894 813 21
SapI GCTCTTCn'nnn_
Cuts at: 0 1010 1728
Size: 1010 718
SbfI CC_TGCA'GG
Cuts at: 0 187 1175 1728
Size: 187 988 553
Fragments arranged by size:
988 553 187
SfcI C'TryA_G
Cuts at: 0 183 349 835 929 1171 1728
Size: 183 166 486 94 242 557
Fragments arranged by size:
557 486 242 183 166 94
SmlI C'TyrA_G
Cuts at: 0 1486 1705 1728
Size: 1486 219 23
Fragments arranged by size:
1486 219 23
SphI G_CATG'C
Cuts at: 0 315 1125 1544 1728
Size: 315 810 419 184
Fragments arranged by size:
810 419 315 184
StuI AGG'CCT
Cuts at: 0 487 1117 1291 1728
Size: 487 630 174 437
Fragments arranged by size:
630 487 437 174
StyI C'CwwG_G
Cuts at: 0 10 317 665 1728
Size: 10 307 348 1063
Fragments arranged by size:
1063 348 307 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 648 658 1432 1728
Size: 153 495 10 774 296
Fragments arranged by size:
774 495 296 153 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 196 228 1656 1688 1728
Size: 196 32 1428 32 40
Fragments arranged by size:
1428 196 40 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1387 1728
Size: 1387 341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 261 1728
Size: 162 99 1467
Fragments arranged by size:
1467 162 99
ZraI GAC'GTC
Cuts at: 0 1701 1728
Size: 1701 27
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BglI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FseI HaeII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PciI PflMI PmlI
PpuMI PspOMI PstI PvuII SapI SbfI SfcI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BpmI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII
DrdI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XhoI XmaI XmnI