Restriction map for
pF1KB6010
(Linear) (Six-base) MAPSORT of: KIBB6010.seq Check: 4451 from: 1 to: 3693
KIBB6010.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 0 2443 3693
Size: 2443 1250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 805 1293 2376 3299 3649 3693
Size: 467 338 488 1083 923 350 44
Fragments arranged by size:
1083 923 488 467 350 338 44
AflIII A'CryG_T
Cuts at: 0 2423 3693
Size: 2423 1270
AhdI GACnn_n'nnGTC
Cuts at: 0 3168 3693
Size: 3168 525
AleI CACnn'nnGTG
Cuts at: 0 2202 3693
Size: 2202 1491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1571 1844 3693
Size: 1571 273 1849
Fragments arranged by size:
1849 1571 273
ApaI G_GGCC'C
Cuts at: 0 148 3693
Size: 148 3545
ApoI r'AATT_y
Cuts at: 0 451 529 908 1288 1990 2586 2735 2935
Size: 451 78 379 380 702 596 149 200
Cuts at: 2935 2985 3013 3135 3693
Size: 50 28 122 558
Fragments arranged by size:
702 596 558 451 380 379 200 149
122 78 50 28
AseI AT'TA_AT
Cuts at: 0 716 3693
Size: 716 2977
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1865 1898 3693
Size: 1865 33 1795
Fragments arranged by size:
1865 1795 33
BanII G_rGCy'C
Cuts at: 0 148 3386 3693
Size: 148 3238 307
Fragments arranged by size:
3238 307 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 1362 1666 2490 3693
Size: 1362 304 824 1203
Fragments arranged by size:
1362 1203 824 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 902 936 3693
Size: 902 34 2757
Fragments arranged by size:
2757 902 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 585 3693
Size: 585 3108
BclI T'GATC_A
Cuts at: 0 2883 3693
Size: 2883 810
BfrBI ATG'CAT
Cuts at: 0 1008 2050 3693
Size: 1008 1042 1643
Fragments arranged by size:
1643 1042 1008
BlpI GC'TnA_GC
Cuts at: 0 2476 3693
Size: 2476 1217
Bme1580I G_kGCm'C
Cuts at: 0 148 3693
Size: 148 3545
BmrI ACTGGGnnnn_n'
Cuts at: 0 1491 3693
Size: 1491 2202
BmtI G_CTAG'C
Cuts at: 0 3148 3693
Size: 3148 545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1309 3693
Size: 1309 2384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 3693
Size: 1304 2389
Bpu10I CC'TnA_GC
Cuts at: 0 2177 3693
Size: 2177 1516
BsaBI GATnn'nnATC
Cuts at: 0 32 2051 3464 3693
Size: 32 2019 1413 229
Fragments arranged by size:
2019 1413 229 32
BsaWI w'CCGG_w
Cuts at: 0 1847 3693
Size: 1847 1846
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 863 893 3693
Size: 863 30 2800
Fragments arranged by size:
2800 863 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 1342 3693
Size: 191 1151 2351
Fragments arranged by size:
2351 1151 191
BseYI C'CCAG_C
Cuts at: 0 147 3693
Size: 147 3546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2549 2557 3313 3693
Size: 2549 8 756 380
Fragments arranged by size:
2549 756 380 8
BsiHKAI G_wGCw'C
Cuts at: 0 1560 3693
Size: 1560 2133
BsmI GAATG_Cn'
Cuts at: 0 861 3693
Size: 861 2832
Bsp1286I G_dGCh'C
Cuts at: 0 148 1560 3386 3693
Size: 148 1412 1826 307
Fragments arranged by size:
1826 1412 307 148
BspEI T'CCGG_A
Cuts at: 0 1847 3693
Size: 1847 1846
BspHI T'CATG_A
Cuts at: 0 3074 3413 3693
Size: 3074 339 280
Fragments arranged by size:
3074 339 280
BsrDI GCAATG_nn'
Cuts at: 0 559 2458 2906 3693
Size: 559 1899 448 787
Fragments arranged by size:
1899 787 559 448
BsrFI r'CCGG_y
Cuts at: 0 1262 1997 3693
Size: 1262 735 1696
Fragments arranged by size:
1696 1262 735
BsrGI T'GTAC_A
Cuts at: 0 879 3693
Size: 879 2814
BstBI TT'CG_AA
Cuts at: 0 435 3693
Size: 435 3258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 229 3693
Size: 229 3464
BstYI r'GATC_y
Cuts at: 0 1320 1844 3549 3693
Size: 1320 524 1705 144
Fragments arranged by size:
1705 1320 524 144
BstZ17I GTA'TAC
Cuts at: 0 2444 3693
Size: 2444 1249
Bsu36I CC'TnA_GG
Cuts at: 0 3401 3693
Size: 3401 292
BtgI C'CryG_G
Cuts at: 0 485 1613 1703 3578 3693
Size: 485 1128 90 1875 115
Fragments arranged by size:
1875 1128 485 115 90
BtsI GCAGTG_nn'
Cuts at: 0 1214 2081 2197 2209 3325 3693
Size: 1214 867 116 12 1116 368
Fragments arranged by size:
1214 1116 867 368 116 12
DraI TTT'AAA
Cuts at: 0 867 986 2282 2946 2984 3693
Size: 867 119 1296 664 38 709
Fragments arranged by size:
1296 867 709 664 119 38
DraIII CAC_nnn'GTG
Cuts at: 0 1522 3693
Size: 1522 2171
DrdI GACnn_nn'nnGTC
Cuts at: 0 631 1037 3693
Size: 631 406 2656
Fragments arranged by size:
2656 631 406
EaeI y'GGCC_r
Cuts at: 0 1264 3693
Size: 1264 2429
EarI CTCTTCn'nnn_
Cuts at: 0 923 1312 1344 1505 1614 1919 2068 3093
Size: 923 389 32 161 109 305 149 1025
Cuts at: 3093 3494 3693
Size: 401 199
Fragments arranged by size:
1025 923 401 389 305 199 161 149
109 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 805 1293 1304 2376 3299 3649 3693
Size: 467 338 488 11 1072 923 350 44
Fragments arranged by size:
1072 923 488 467 350 338 44 11
EcoO109I rG'GnC_Cy
Cuts at: 0 144 3693
Size: 144 3549
EcoRI G'AATT_C
Cuts at: 0 451 529 3693
Size: 451 78 3164
Fragments arranged by size:
3164 451 78
EcoRV GAT'ATC
Cuts at: 0 87 2652 2694 3693
Size: 87 2565 42 999
Fragments arranged by size:
2565 999 87 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1534 2286 3693
Size: 1534 752 1407
Fragments arranged by size:
1534 1407 752
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 2681 2713 2806 2838 3154 3186 3408
Size: 26 2655 32 93 32 316 32 222
Cuts at: 3408 3426 3440 3458 3534 3566 3693
Size: 18 14 18 76 32 127
Fragments arranged by size:
2655 316 222 127 93 76 32 32
32 32 26 18 18 14
HincII GTy'rAC
Cuts at: 0 1966 2066 2317 3203 3693
Size: 1966 100 251 886 490
Fragments arranged by size:
1966 886 490 251 100
MfeI C'AATT_G
Cuts at: 0 3506 3693
Size: 3506 187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1649 3693
Size: 979 670 2044
Fragments arranged by size:
2044 979 670
MslI CAynn'nnrTG
Cuts at: 0 538 718 943 1424 1826 2202 2911 2924
Size: 538 180 225 481 402 376 709 13
Cuts at: 2924 3373 3693
Size: 449 320
Fragments arranged by size:
709 538 481 449 402 376 320 225
180 13
MspA1I CmG'CkG
Cuts at: 0 1282 2644 3693
Size: 1282 1362 1049
Fragments arranged by size:
1362 1282 1049
NcoI C'CATG_G
Cuts at: 0 485 3578 3693
Size: 485 3093 115
Fragments arranged by size:
3093 485 115
NdeI CA'TA_TG
Cuts at: 0 413 3693
Size: 413 3280
NheI G'CTAG_C
Cuts at: 0 3144 3693
Size: 3144 549
NsiI A_TGCA'T
Cuts at: 0 1010 2052 3693
Size: 1010 1042 1641
Fragments arranged by size:
1641 1042 1010
NspI r_CATG'y
Cuts at: 0 1429 1452 1895 2175 2427 3264 3693
Size: 1429 23 443 280 252 837 429
Fragments arranged by size:
1429 837 443 429 280 252 23
PciI A'CATG_T
Cuts at: 0 2423 3693
Size: 2423 1270
PflMI CCAn_nnn'nTGG
Cuts at: 0 1522 3693
Size: 1522 2171
PsiI TTA'TAA
Cuts at: 0 1067 2678 3464 3693
Size: 1067 1611 786 229
Fragments arranged by size:
1611 1067 786 229
PspOMI G'GGCC_C
Cuts at: 0 144 3693
Size: 144 3549
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 785 817 3693
Size: 785 32 2876
Fragments arranged by size:
2876 785 32
PstI C_TGCA'G
Cuts at: 0 1221 2204 2649 3693
Size: 1221 983 445 1044
Fragments arranged by size:
1221 1044 983 445
PvuII CAG'CTG
Cuts at: 0 1282 2644 3693
Size: 1282 1362 1049
Fragments arranged by size:
1362 1282 1049
SapI GCTCTTCn'nnn_
Cuts at: 0 1312 1505 3494 3693
Size: 1312 193 1989 199
Fragments arranged by size:
1989 1312 199 193
ScaI AGT'ACT
Cuts at: 0 1780 3693
Size: 1780 1913
SfcI C'TryA_G
Cuts at: 0 40 1217 1736 2200 2645 3233 3693
Size: 40 1177 519 464 445 588 460
Fragments arranged by size:
1177 588 519 464 460 445 40
SphI G_CATG'C
Cuts at: 0 1429 3264 3693
Size: 1429 1835 429
Fragments arranged by size:
1835 1429 429
SspI AAT'ATT
Cuts at: 0 863 1854 3693
Size: 863 991 1839
Fragments arranged by size:
1839 991 863
StuI AGG'CCT
Cuts at: 0 2359 3693
Size: 2359 1334
StyI C'CwwG_G
Cuts at: 0 485 3578 3693
Size: 485 3093 115
Fragments arranged by size:
3093 485 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 341 3693
Size: 341 3352
TatI w'GTAC_w
Cuts at: 0 879 1161 1778 2206 3693
Size: 879 282 617 428 1487
Fragments arranged by size:
1487 879 617 428 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 1622 1654 3693
Size: 1435 32 155 32 2039
Fragments arranged by size:
2039 1435 155 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1361 3693
Size: 1361 2332
XbaI T'CTAG_A
Cuts at: 0 1404 3693
Size: 1404 2289
XmnI GAAnn'nnTTC
Cuts at: 0 508 1208 2012 3671 3693
Size: 508 700 804 1659 22
Fragments arranged by size:
1659 804 700 508 22
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI ApaI ApoI
AseI BaeI BanII BbsI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrDI BsrFI BsrGI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
MfeI MmeI MslI MspA1I NcoI NdeI NheI NsiI
NspI PciI PflMI PsiI PspOMI PsrI PstI PvuII
SapI ScaI SfcI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
AlwNI ApaLI AscI AsiSI AvaI AvrII BamHI BanI
BbeI BbvCI BglI BglII BmgBI BpuEI BsaI BsaAI
BsaHI BsiEI BsiWI BsmBI BspMI BsrBI BssHII BssSI
BstAPI BstEII BtgZI ClaI CspCI EagI EciI EcoICRI
EcoNI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NgoMIV NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SwaI XcmI XhoI XmaI ZraI