Restriction map for
pF1KB6006
(Linear) (Six-base) MAPSORT of: KIBB6006.seq Check: 8927 from: 1 to: 7404
KIBB6006.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 161 7239 7404
Size: 161 7078 165
Fragments arranged by size:
7078 165 161
AccI GT'mk_AC
Cuts at: 0 2759 3146 3364 3409 4989 7404
Size: 2759 387 218 45 1580 2415
Fragments arranged by size:
2759 2415 1580 387 218 45
Acc65I G'GTAC_C
Cuts at: 0 1327 7295 7404
Size: 1327 5968 109
Fragments arranged by size:
5968 1327 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 593 784 1385 1552 1654 1784 1958
Size: 296 297 191 601 167 102 130 174
Cuts at: 1958 2261 2555 2620 2717 2792 3068 3098 3143
Size: 303 294 65 97 75 276 30 45
Cuts at: 3143 3581 3590 3707 4109 4586 4968 5075 5819
Size: 438 9 117 402 477 382 107 744
Cuts at: 5819 5870 6080 6167 6368 6518 6560 7404
Size: 51 210 87 201 150 42 844
Fragments arranged by size:
844 744 601 477 438 402 382 303
297 296 294 276 210 201 191 174
167 150 130 117 107 102 97 87
75 65 51 45 42 30 9
AflIII A'CryG_T
Cuts at: 0 2880 4622 7404
Size: 2880 1742 2782
Fragments arranged by size:
2880 2782 1742
AhdI GACnn_n'nnGTC
Cuts at: 0 4161 5667 7404
Size: 4161 1506 1737
Fragments arranged by size:
4161 1737 1506
AleI CACnn'nnGTG
Cuts at: 0 308 367 1582 1708 2885 7404
Size: 308 59 1215 126 1177 4519
Fragments arranged by size:
4519 1215 1177 308 126 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 1315 1684 4988 7261 7404
Size: 385 930 369 3304 2273 143
Fragments arranged by size:
3304 2273 930 385 369 143
AlwNI CAG_nnn'CTG
Cuts at: 0 1542 1807 2380 2419 2815 2842 3163 4336
Size: 1542 265 573 39 396 27 321 1173
Cuts at: 4336 4777 6690 7404
Size: 441 1913 714
Fragments arranged by size:
1913 1542 1173 714 573 441 396 321
265 39 27
ApoI r'AATT_y
Cuts at: 0 1348 2773 3334 3472 3649 4090 4942 7123
Size: 1348 1425 561 138 177 441 852 2181
Cuts at: 7123 7404
Size: 281
Fragments arranged by size:
2181 1425 1348 852 561 441 281 177
138
AseI AT'TA_AT
Cuts at: 0 6305 7404
Size: 6305 1099
AvaI C'yCGr_G
Cuts at: 0 991 3416 7404
Size: 991 2425 3988
Fragments arranged by size:
3988 2425 991
BamHI G'GATC_C
Cuts at: 0 4213 7404
Size: 4213 3191
BanI G'GyrC_C
Cuts at: 0 1327 1572 3169 4270 7295 7404
Size: 1327 245 1597 1101 3025 109
Fragments arranged by size:
3025 1597 1327 1101 245 109
BanII G_rGCy'C
Cuts at: 0 182 449 992 1015 2741 3222 3389 3769
Size: 182 267 543 23 1726 481 167 380
Cuts at: 3769 4148 4681 5159 5486 6387 7190 7404
Size: 379 533 478 327 901 803 214
Fragments arranged by size:
1726 901 803 543 533 481 478 380
379 327 267 214 182 167 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1912 1946 2591 3011 3131 3326 3650 4415
Size: 1912 34 645 420 120 195 324 765
Cuts at: 4415 4550 4625 5593 5686 5839 6548 6730 7136
Size: 135 75 968 93 153 709 182 406
Cuts at: 7136 7404
Size: 268
Fragments arranged by size:
1912 968 765 709 645 420 406 324
268 195 182 153 135 120 93 75
34
BbvCI CC'TCA_GC
Cuts at: 0 2567 2852 4727 7181 7404
Size: 2567 285 1875 2454 223
Fragments arranged by size:
2567 2454 1875 285 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3136 3170 6971 7005 7404
Size: 3136 34 3801 34 399
Fragments arranged by size:
3801 3136 399 34 34
BclI T'GATC_A
Cuts at: 0 335 380 7404
Size: 335 45 7024
Fragments arranged by size:
7024 335 45
BfrBI ATG'CAT
Cuts at: 0 2950 7404
Size: 2950 4454
BglI GCCn_nnn'nGGC
Cuts at: 0 58 1858 2377 4669 7404
Size: 58 1800 519 2292 2735
Fragments arranged by size:
2735 2292 1800 519 58
BglII A'GATC_T
Cuts at: 0 1377 3658 4915 6711 7323 7404
Size: 1377 2281 1257 1796 612 81
Fragments arranged by size:
2281 1796 1377 1257 612 81
Bme1580I G_kGCm'C
Cuts at: 0 1577 6508 7052 7404
Size: 1577 4931 544 352
Fragments arranged by size:
4931 1577 544 352
BmgBI CAC'GTC
Cuts at: 0 3423 7404
Size: 3423 3981
BmrI ACTGGGnnnn_n'
Cuts at: 0 1476 3216 7404
Size: 1476 1740 4188
Fragments arranged by size:
4188 1740 1476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 714 3800 4752 5178 5347 5349 6525 7404
Size: 714 3086 952 426 169 2 1176 879
Fragments arranged by size:
3086 1176 952 879 714 426 169 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1138 1826 2423 2593 2755 2837 3293
Size: 439 699 688 597 170 162 82 456
Cuts at: 3293 4298 4982 5073 5587 6380 6587 7404
Size: 1005 684 91 514 793 207 817
Fragments arranged by size:
1005 817 793 699 688 684 597 514
456 439 207 170 162 91 82
Bpu10I CC'TnA_GC
Cuts at: 0 2567 2852 4727 4961 7181 7404
Size: 2567 285 1875 234 2220 223
Fragments arranged by size:
2567 2220 1875 285 234 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 6340 7404
Size: 1279 5061 1064
Fragments arranged by size:
5061 1279 1064
BsaI GGTCTCn'nnnn_
Cuts at: 0 1783 3430 5764 6949 7297 7404
Size: 1783 1647 2334 1185 348 107
Fragments arranged by size:
2334 1783 1647 1185 348 107
BsaAI yAC'GTr
Cuts at: 0 1719 2883 3670 4625 6581 6934 7155 7404
Size: 1719 1164 787 955 1956 353 221 249
Fragments arranged by size:
1956 1719 1164 955 787 353 249 221
BsaBI GATnn'nnATC
Cuts at: 0 5144 7404
Size: 5144 2260
BsaHI Gr'CG_yC
Cuts at: 0 64 3844 7404
Size: 64 3780 3560
Fragments arranged by size:
3780 3560 64
BsaWI w'CCGG_w
Cuts at: 0 439 7404
Size: 439 6965
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 599 629 1033 1063 5110 5140 5727 5757
Size: 599 30 404 30 4047 30 587 30
Cuts at: 5757 6897 6927 7404
Size: 1140 30 477
Fragments arranged by size:
4047 1140 599 587 477 404 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1966 2222 2743 2860 2869 3067 3079
Size: 160 1806 256 521 117 9 198 12
Cuts at: 3079 3106 3190 3322 5134 5219 5252 7198 7404
Size: 27 84 132 1812 85 33 1946 206
Fragments arranged by size:
1946 1812 1806 521 256 206 198 160
132 117 85 84 33 27 12 9
BseYI C'CCAG_C
Cuts at: 0 1822 6010 7189 7404
Size: 1822 4188 1179 215
Fragments arranged by size:
4188 1822 1179 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5039 6914 7404
Size: 5039 1875 490
Fragments arranged by size:
5039 1875 490
BsiEI CG_ry'CG
Cuts at: 0 8 6521 7404
Size: 8 6513 883
Fragments arranged by size:
6513 883 8
BsiHKAI G_wGCw'C
Cuts at: 0 152 182 449 992 4681 5159 7404
Size: 152 30 267 543 3689 478 2245
Fragments arranged by size:
3689 2245 543 478 267 152 30
BsmI GAATG_Cn'
Cuts at: 0 220 4840 6969 7404
Size: 220 4620 2129 435
Fragments arranged by size:
4620 2129 435 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3413 3445 7404
Size: 3413 32 3959
Fragments arranged by size:
3959 3413 32
Bsp1286I G_dGCh'C
Cuts at: 0 152 182 449 992 1015 1577 2741 3222
Size: 152 30 267 543 23 562 1164 481
Cuts at: 3222 3389 3769 4148 4681 5159 5486 6387 6508
Size: 167 380 379 533 478 327 901 121
Cuts at: 6508 7052 7190 7404
Size: 544 138 214
Fragments arranged by size:
1164 901 562 544 543 533 481 478
380 379 327 267 214 167 152 138
121 30 23
BspHI T'CATG_A
Cuts at: 0 3255 7269 7404
Size: 3255 4014 135
Fragments arranged by size:
4014 3255 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 3249 3537 7239 7404
Size: 161 3088 288 3702 165
Fragments arranged by size:
3702 3088 288 165 161
BsrBI CCG'CTC
Cuts at: 0 3546 7404
Size: 3546 3858
BsrDI GCAATG_nn'
Cuts at: 0 5970 7404
Size: 5970 1434
BsrFI r'CCGG_y
Cuts at: 0 2791 7404
Size: 2791 4613
BssSI C'ACGA_G
Cuts at: 0 409 5366 6250 7404
Size: 409 4957 884 1154
Fragments arranged by size:
4957 1154 884 409
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1438 7404
Size: 1438 5966
BstEII G'GTnAC_C
Cuts at: 0 3900 7404
Size: 3900 3504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1584 3229 3396 6001 7404
Size: 1584 1645 167 2605 1403
Fragments arranged by size:
2605 1645 1584 1403 167
BstYI r'GATC_y
Cuts at: 0 293 1377 1557 2502 3658 4213 4531 4915
Size: 293 1084 180 945 1156 555 318 384
Cuts at: 4915 6711 7323 7404
Size: 1796 612 81
Fragments arranged by size:
1796 1156 1084 945 612 555 384 318
293 180 81
Bsu36I CC'TnA_GG
Cuts at: 0 1697 2498 2726 3329 3896 3938 5624 6155
Size: 1697 801 228 603 567 42 1686 531
Cuts at: 6155 6917 7404
Size: 762 487
Fragments arranged by size:
1697 1686 801 762 603 567 531 487
228 42
BtgI C'CryG_G
Cuts at: 0 8 3395 3635 4136 6653 7404
Size: 8 3387 240 501 2517 751
Fragments arranged by size:
3387 2517 751 501 240 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 7404
Size: 33 7371
BtsI GCAGTG_nn'
Cuts at: 0 430 833 1324 3338 3993 6197 7004 7404
Size: 430 403 491 2014 655 2204 807 400
Fragments arranged by size:
2204 2014 807 655 491 430 403 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5039 5074 7404
Size: 5039 35 2330
Fragments arranged by size:
5039 2330 35
DraI TTT'AAA
Cuts at: 0 2264 4539 7404
Size: 2264 2275 2865
Fragments arranged by size:
2865 2275 2264
DraIII CAC_nnn'GTG
Cuts at: 0 5596 7404
Size: 5596 1808
DrdI GACnn_nn'nnGTC
Cuts at: 0 5934 6574 7035 7404
Size: 5934 640 461 369
Fragments arranged by size:
5934 640 461 369
EaeI y'GGCC_r
Cuts at: 0 1544 1566 2978 3231 4416 4947 5991 6518
Size: 1544 22 1412 253 1185 531 1044 527
Cuts at: 6518 6674 6992 7404
Size: 156 318 412
Fragments arranged by size:
1544 1412 1185 1044 531 527 412 318
253 156 22
EagI C'GGCC_G
Cuts at: 0 6518 7404
Size: 6518 886
EarI CTCTTCn'nnn_
Cuts at: 0 1117 1826 2027 2066 2141 2282 2516 2531
Size: 1117 709 201 39 75 141 234 15
Cuts at: 2531 2585 2822 2858 2959 3044 3443 3636 3757
Size: 54 237 36 101 85 399 193 121
Cuts at: 3757 5202 5457 5846 6494 6935 7404
Size: 1445 255 389 648 441 469
Fragments arranged by size:
1445 1117 709 648 469 441 399 389
255 237 234 201 193 141 121 101
85 75 54 39 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 872 2953 7404
Size: 872 2081 4451
Fragments arranged by size:
4451 2081 872
EcoICRI GAG'CTC
Cuts at: 0 180 447 990 4679 5157 7404
Size: 180 267 543 3689 478 2247
Fragments arranged by size:
3689 2247 543 478 267 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 439 593 784 1138 1385 1552 1654
Size: 296 143 154 191 354 247 167 102
Cuts at: 1654 1784 1826 1958 2261 2423 2555 2593 2620
Size: 130 42 132 303 162 132 38 27
Cuts at: 2620 2717 2755 2792 2837 3068 3098 3143 3293
Size: 97 38 37 45 231 30 45 150
Cuts at: 3293 3581 3590 3707 4109 4298 4586 4968 4982
Size: 288 9 117 402 189 288 382 14
Cuts at: 4982 5073 5075 5587 5819 5870 6080 6167 6368
Size: 91 2 512 232 51 210 87 201
Cuts at: 6368 6380 6518 6560 6587 7404
Size: 12 138 42 27 817
Fragments arranged by size:
817 512 402 382 354 303 296 288
288 247 232 231 210 201 191 189
167 162 154 150 143 138 132 132
130 117 102 97 91 87 51 45
45 42 42 38 38 37 30 27
27 14 12 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 575 1058 5569 6398 7404
Size: 575 483 4511 829 1006
Fragments arranged by size:
4511 1006 829 575 483
EcoO109I rG'GnC_Cy
Cuts at: 0 2480 2659 5639 6006 7404
Size: 2480 179 2980 367 1398
Fragments arranged by size:
2980 2480 1398 367 179
EcoRI G'AATT_C
Cuts at: 0 1348 3334 3472 4090 7404
Size: 1348 1986 138 618 3314
Fragments arranged by size:
3314 1986 1348 618 138
EcoRV GAT'ATC
Cuts at: 0 3377 5349 7404
Size: 3377 1972 2055
Fragments arranged by size:
3377 2055 1972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 7404
Size: 129 7275
FspI TGC'GCA
Cuts at: 0 1672 7404
Size: 1672 5732
HaeII r_GCGC'y
Cuts at: 0 2897 7404
Size: 2897 4507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 285 310 317 599 631 856 888
Size: 278 7 25 7 282 32 225 32
Cuts at: 888 3391 3423 3442 3474 3712 3744 4015 4047
Size: 2503 32 19 32 238 32 271 32
Cuts at: 4047 4147 4179 4612 4644 4693 4725 4900 4932
Size: 100 32 433 32 49 32 175 32
Cuts at: 4932 5110 5142 5211 5233 5243 5265 5395 5427
Size: 178 32 69 22 10 22 130 32
Cuts at: 5427 5458 5490 5653 5685 6775 6807 6835 6867
Size: 31 32 163 32 1090 32 28 32
Cuts at: 6867 7251 7283 7404
Size: 384 32 121
Fragments arranged by size:
2503 1090 433 384 282 278 271 238
225 178 175 163 130 121 100 69
49 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 31 28 25 22 22 19
10 7 7
HincII GTy'rAC
Cuts at: 0 3147 4990 7404
Size: 3147 1843 2414
Fragments arranged by size:
3147 2414 1843
KpnI G_GTAC'C
Cuts at: 0 1331 7299 7404
Size: 1331 5968 105
Fragments arranged by size:
5968 1331 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1484 6530 7196 7404
Size: 918 566 5046 666 208
Fragments arranged by size:
5046 918 666 566 208
MscI TGG'CCA
Cuts at: 0 1546 1568 2980 4418 4949 5993 6676 7404
Size: 1546 22 1412 1438 531 1044 683 728
Fragments arranged by size:
1546 1438 1412 1044 728 683 531 22
MslI CAynn'nnrTG
Cuts at: 0 308 367 1117 1582 1708 2885 3394 4552
Size: 308 59 750 465 126 1177 509 1158
Cuts at: 4552 6644 7249 7404
Size: 2092 605 155
Fragments arranged by size:
2092 1177 1158 750 605 509 465 308
155 126 59
MspA1I CmG'CkG
Cuts at: 0 975 2257 2392 2419 2434 2571 3733 6823
Size: 975 1282 135 27 15 137 1162 3090
Cuts at: 6823 7404
Size: 581
Fragments arranged by size:
3090 1282 1162 975 581 137 135 27
15
NcoI C'CATG_G
Cuts at: 0 3395 3635 4136 6653 7404
Size: 3395 240 501 2517 751
Fragments arranged by size:
3395 2517 751 501 240
NdeI CA'TA_TG
Cuts at: 0 4634 5868 7404
Size: 4634 1234 1536
Fragments arranged by size:
4634 1536 1234
NsiI A_TGCA'T
Cuts at: 0 2952 7404
Size: 2952 4452
NspI r_CATG'y
Cuts at: 0 969 4726 5082 7248 7391 7404
Size: 969 3757 356 2166 143 13
Fragments arranged by size:
3757 2166 969 356 143 13
PasI CC'CwG_GG
Cuts at: 0 1008 3813 7404
Size: 1008 2805 3591
Fragments arranged by size:
3591 2805 1008
PflMI CCAn_nnn'nTGG
Cuts at: 0 1242 7404
Size: 1242 6162
PfoI T'CCnGG_A
Cuts at: 0 795 1493 4802 4891 6396 7202 7404
Size: 795 698 3309 89 1505 806 202
Fragments arranged by size:
3309 1505 806 795 698 202 89
PmlI CAC'GTG
Cuts at: 0 1719 6934 7404
Size: 1719 5215 470
Fragments arranged by size:
5215 1719 470
PpuMI rG'GwC_Cy
Cuts at: 0 2480 2659 5639 6006 7404
Size: 2480 179 2980 367 1398
Fragments arranged by size:
2980 2480 1398 367 179
PshAI GACnn'nnGTC
Cuts at: 0 4848 4985 5066 7404
Size: 4848 137 81 2338
Fragments arranged by size:
4848 2338 137 81
PspXI vC'TCGA_Gb
Cuts at: 0 991 7404
Size: 991 6413
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6090 6122 7404
Size: 6090 32 1282
Fragments arranged by size:
6090 1282 32
PstI C_TGCA'G
Cuts at: 0 2391 3159 3870 5418 6135 6204 7011 7404
Size: 2391 768 711 1548 717 69 807 393
Fragments arranged by size:
2391 1548 807 768 717 711 393 69
PvuII CAG'CTG
Cuts at: 0 2257 2392 2419 2434 2571 3733 7404
Size: 2257 135 27 15 137 1162 3671
Fragments arranged by size:
3671 2257 1162 137 135 27 15
SacI G_AGCT'C
Cuts at: 0 182 449 992 4681 5159 7404
Size: 182 267 543 3689 478 2245
Fragments arranged by size:
3689 2245 543 478 267 182
SalI G'TCGA_C
Cuts at: 0 3145 4988 7404
Size: 3145 1843 2416
Fragments arranged by size:
3145 2416 1843
SanDI GG'GwC_CC
Cuts at: 0 6006 7404
Size: 6006 1398
SapI GCTCTTCn'nnn_
Cuts at: 0 1826 3757 7404
Size: 1826 1931 3647
Fragments arranged by size:
3647 1931 1826
SexAI A'CCwGG_T
Cuts at: 0 5592 5635 7404
Size: 5592 43 1769
Fragments arranged by size:
5592 1769 43
SfcI C'TryA_G
Cuts at: 0 2387 3155 3866 4046 5009 5064 5414 6131
Size: 2387 768 711 180 963 55 350 717
Cuts at: 6131 6200 7007 7404
Size: 69 807 397
Fragments arranged by size:
2387 963 807 768 717 711 397 350
180 69 55
SmlI C'TyrA_G
Cuts at: 0 991 1258 3416 6319 7404
Size: 991 267 2158 2903 1085
Fragments arranged by size:
2903 2158 1085 991 267
SpeI A'CTAG_T
Cuts at: 0 4351 7404
Size: 4351 3053
SphI G_CATG'C
Cuts at: 0 4726 7391 7404
Size: 4726 2665 13
Fragments arranged by size:
4726 2665 13
SspI AAT'ATT
Cuts at: 0 633 7404
Size: 633 6771
StuI AGG'CCT
Cuts at: 0 5182 6670 7058 7404
Size: 5182 1488 388 346
Fragments arranged by size:
5182 1488 388 346
StyI C'CwwG_G
Cuts at: 0 1898 2510 2933 3395 3635 4136 6653 6671
Size: 1898 612 423 462 240 501 2517 18
Cuts at: 6671 6800 7404
Size: 129 604
Fragments arranged by size:
2517 1898 612 604 501 462 423 240
129 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 547 920 943 1495 1564 1746 3008
Size: 157 390 373 23 552 69 182 1262
Cuts at: 3008 3805 4860 5005 5149 5692 6487 7404
Size: 797 1055 145 144 543 795 917
Fragments arranged by size:
1262 1055 917 797 795 552 543 390
373 182 157 145 144 69 23
TatI w'GTAC_w
Cuts at: 0 1484 7404
Size: 1484 5920
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 2643 2675 5727 5759 6897 6929
Size: 519 32 2092 32 3052 32 1138 32
Cuts at: 6929 7404
Size: 475
Fragments arranged by size:
3052 2092 1138 519 475 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3456 6756 7035 7404
Size: 3456 3300 279 369
Fragments arranged by size:
3456 3300 369 279
XbaI T'CTAG_A
Cuts at: 0 1521 5938 7404
Size: 1521 4417 1466
Fragments arranged by size:
4417 1521 1466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3705 4665 5562 7404
Size: 3705 960 897 1842
Fragments arranged by size:
3705 1842 960 897
XhoI C'TCGA_G
Cuts at: 0 991 3416 7404
Size: 991 2425 3988
Fragments arranged by size:
3988 2425 991
XmnI GAAnn'nnTTC
Cuts at: 0 835 1352 4235 7404
Size: 835 517 2883 3169
Fragments arranged by size:
3169 2883 835 517
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspXI PsrI PstI PvuII SacI
SalI SanDI SapI SexAI SfcI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AloI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BciVI BlpI BmtI
BsiWI BspEI BsrGI BssHII BstBI BstZ17I ClaI FseI
FspAI HindIII HpaI KasI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PciI PmeI PpiI
PsiI PspOMI PvuI RsrII SacII SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XmaI
ZraI