Restriction map for
pF1KB5979
(Linear) (Six-base) MAPSORT of: KIBB5979.seq Check: 7631 from: 1 to: 1560
KIBB5979.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1298 1560
Size: 749 549 262
Fragments arranged by size:
749 549 262
AfeI AGC'GCT
Cuts at: 0 150 1560
Size: 150 1410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1410 1560
Size: 1410 150
ApaI G_GGCC'C
Cuts at: 0 993 1560
Size: 993 567
ApaLI G'TGCA_C
Cuts at: 0 670 1560
Size: 670 890
AvaI C'yCGr_G
Cuts at: 0 14 552 665 1560
Size: 14 538 113 895
Fragments arranged by size:
895 538 113 14
BamHI G'GATC_C
Cuts at: 0 396 1560
Size: 396 1164
BanI G'GyrC_C
Cuts at: 0 432 1560
Size: 432 1128
BanII G_rGCy'C
Cuts at: 0 13 197 993 1560
Size: 13 184 796 567
Fragments arranged by size:
796 567 184 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1286 1560
Size: 1286 274
BciVI GTATCCnnnnn_n'
Cuts at: 0 1547 1560
Size: 1547 13
BclI T'GATC_A
Cuts at: 0 335 1290 1560
Size: 335 955 270
Fragments arranged by size:
955 335 270
BfrBI ATG'CAT
Cuts at: 0 651 1560
Size: 651 909
Bme1580I G_kGCm'C
Cuts at: 0 674 993 1560
Size: 674 319 567
Fragments arranged by size:
674 567 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1269 1560
Size: 953 316 291
Fragments arranged by size:
953 316 291
Bpu10I CC'TnA_GC
Cuts at: 0 98 1560
Size: 98 1462
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 553 880 1560
Size: 45 508 327 680
Fragments arranged by size:
680 508 327 45
BsaAI yAC'GTr
Cuts at: 0 675 1560
Size: 675 885
BsaBI GATnn'nnATC
Cuts at: 0 1295 1560
Size: 1295 265
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 148 1560
Size: 145 3 1412
Fragments arranged by size:
1412 145 3
BseYI C'CCAG_C
Cuts at: 0 55 490 1560
Size: 55 435 1070
Fragments arranged by size:
1070 435 55
BsiHKAI G_wGCw'C
Cuts at: 0 13 674 1560
Size: 13 661 886
Fragments arranged by size:
886 661 13
BsmI GAATG_Cn'
Cuts at: 0 1073 1560
Size: 1073 487
Bsp1286I G_dGCh'C
Cuts at: 0 13 197 674 993 1560
Size: 13 184 477 319 567
Fragments arranged by size:
567 477 319 184 13
BspHI T'CATG_A
Cuts at: 0 1217 1560
Size: 1217 343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1097 1112 1560
Size: 1097 15 448
Fragments arranged by size:
1097 448 15
BsrFI r'CCGG_y
Cuts at: 0 435 1164 1560
Size: 435 729 396
Fragments arranged by size:
729 435 396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 1560
Size: 648 912
BstEII G'GTnAC_C
Cuts at: 0 1251 1560
Size: 1251 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1560
Size: 429 1131
BstYI r'GATC_y
Cuts at: 0 292 396 1560
Size: 292 104 1164
Fragments arranged by size:
1164 292 104
BtgI C'CryG_G
Cuts at: 0 32 895 1560
Size: 32 863 665
Fragments arranged by size:
863 665 32
BtsI GCAGTG_nn'
Cuts at: 0 699 963 1560
Size: 699 264 597
Fragments arranged by size:
699 597 264
ClaI AT'CG_AT
Cuts at: 0 425 1560
Size: 425 1135
DraI TTT'AAA
Cuts at: 0 753 924 1560
Size: 753 171 636
Fragments arranged by size:
753 636 171
DraIII CAC_nnn'GTG
Cuts at: 0 949 1560
Size: 949 611
EaeI y'GGCC_r
Cuts at: 0 419 1560
Size: 419 1141
EarI CTCTTCn'nnn_
Cuts at: 0 122 154 257 1560
Size: 122 32 103 1303
Fragments arranged by size:
1303 122 103 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 134 372 1560
Size: 103 31 238 1188
Fragments arranged by size:
1188 238 103 31
EcoICRI GAG'CTC
Cuts at: 0 11 1560
Size: 11 1549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 953 1269 1298 1560
Size: 749 204 316 29 262
Fragments arranged by size:
749 316 262 204 29
EcoO109I rG'GnC_Cy
Cuts at: 0 989 990 1560
Size: 989 1 570
Fragments arranged by size:
989 570 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 842 1560
Size: 842 718
HaeII r_GCGC'y
Cuts at: 0 152 1560
Size: 152 1408
HincII GTy'rAC
Cuts at: 0 699 1101 1560
Size: 699 402 459
Fragments arranged by size:
699 459 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1153 1490 1560
Size: 374 779 337 70
Fragments arranged by size:
779 374 337 70
MscI TGG'CCA
Cuts at: 0 421 1560
Size: 421 1139
MslI CAynn'nnrTG
Cuts at: 0 427 1560
Size: 427 1133
MspA1I CmG'CkG
Cuts at: 0 233 1560
Size: 233 1327
NcoI C'CATG_G
Cuts at: 0 895 1560
Size: 895 665
NsiI A_TGCA'T
Cuts at: 0 653 1560
Size: 653 907
PasI CC'CwG_GG
Cuts at: 0 1245 1560
Size: 1245 315
PmlI CAC'GTG
Cuts at: 0 675 1560
Size: 675 885
PshAI GACnn'nnGTC
Cuts at: 0 1490 1560
Size: 1490 70
PspOMI G'GGCC_C
Cuts at: 0 989 1560
Size: 989 571
PstI C_TGCA'G
Cuts at: 0 156 1560
Size: 156 1404
SacI G_AGCT'C
Cuts at: 0 13 1560
Size: 13 1547
SapI GCTCTTCn'nnn_
Cuts at: 0 154 1560
Size: 154 1406
SfcI C'TryA_G
Cuts at: 0 152 453 1560
Size: 152 301 1107
Fragments arranged by size:
1107 301 152
SspI AAT'ATT
Cuts at: 0 1356 1560
Size: 1356 204
StuI AGG'CCT
Cuts at: 0 903 1560
Size: 903 657
StyI C'CwwG_G
Cuts at: 0 41 895 1062 1560
Size: 41 854 167 498
Fragments arranged by size:
854 498 167 41
TatI w'GTAC_w
Cuts at: 0 656 1560
Size: 656 904
XbaI T'CTAG_A
Cuts at: 0 1447 1560
Size: 1447 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 1136 1560
Size: 612 524 424
Fragments arranged by size:
612 524 424
XmnI GAAnn'nnTTC
Cuts at: 0 310 1560
Size: 310 1250
Enzymes that do cut:
AcuI AfeI AlfI ApaI ApaLI AvaI BamHI BanI
BanII BbsI BciVI BclI BfrBI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrFI BstAPI BstEII BstXI BstYI BtgI
BtsI ClaI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII HincII MmeI MscI MslI
MspA1I NcoI NsiI PasI PmlI PshAI PspOMI PstI
SacI SapI SfcI SspI StuI StyI TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstZ17I Bsu36I BtgZI CspCI DrdI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TaqII TstI Tth111I XhoI
XmaI ZraI