Restriction map for
pF1KB5971
(Linear) (Six-base) MAPSORT of: KIBB5971.seq Check: 2195 from: 1 to: 1554
KIBB5971.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 784 1554
Size: 784 770
AccI GT'mk_AC
Cuts at: 0 1063 1554
Size: 1063 491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 305 1198 1310 1554
Size: 198 107 893 112 244
Fragments arranged by size:
893 244 198 112 107
AflIII A'CryG_T
Cuts at: 0 461 1478 1554
Size: 461 1017 76
Fragments arranged by size:
1017 461 76
AleI CACnn'nnGTG
Cuts at: 0 969 1554
Size: 969 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 975 1554
Size: 975 579
AlwNI CAG_nnn'CTG
Cuts at: 0 77 280 793 1554
Size: 77 203 513 761
Fragments arranged by size:
761 513 203 77
ApaI G_GGCC'C
Cuts at: 0 399 725 1388 1554
Size: 399 326 663 166
Fragments arranged by size:
663 399 326 166
ApaLI G'TGCA_C
Cuts at: 0 972 1554
Size: 972 582
AvaI C'yCGr_G
Cuts at: 0 1088 1493 1554
Size: 1088 405 61
Fragments arranged by size:
1088 405 61
BanI G'GyrC_C
Cuts at: 0 698 796 1000 1554
Size: 698 98 204 554
Fragments arranged by size:
698 554 204 98
BanII G_rGCy'C
Cuts at: 0 176 378 399 725 904 1388 1554
Size: 176 202 21 326 179 484 166
Fragments arranged by size:
484 326 202 179 176 166 21
BbeI G_GCGC'C
Cuts at: 0 800 1554
Size: 800 754
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 1279 1554
Size: 448 831 275
Fragments arranged by size:
831 448 275
BbvCI CC'TCA_GC
Cuts at: 0 1136 1554
Size: 1136 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 100 134 1554
Size: 100 34 1420
Fragments arranged by size:
1420 100 34
BclI T'GATC_A
Cuts at: 0 575 1554
Size: 575 979
BglI GCCn_nnn'nGGC
Cuts at: 0 88 299 498 556 1554
Size: 88 211 199 58 998
Fragments arranged by size:
998 211 199 88 58
Bme1580I G_kGCm'C
Cuts at: 0 399 725 964 976 1388 1554
Size: 399 326 239 12 412 166
Fragments arranged by size:
412 399 326 239 166 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 630 1136 1554
Size: 268 362 506 418
Fragments arranged by size:
506 418 362 268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1262 1554
Size: 121 1141 292
Fragments arranged by size:
1141 292 121
Bpu10I CC'TnA_GC
Cuts at: 0 494 1136 1554
Size: 494 642 418
Fragments arranged by size:
642 494 418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 994 1554
Size: 951 43 560
Fragments arranged by size:
951 560 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 33 1554
Size: 33 1521
BsaHI Gr'CG_yC
Cuts at: 0 797 1554
Size: 797 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 802 832 1501 1531 1554
Size: 802 30 669 30 23
Fragments arranged by size:
802 669 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1506 1554
Size: 391 1115 48
Fragments arranged by size:
1115 391 48
BseYI C'CCAG_C
Cuts at: 0 70 837 1004 1549 1554
Size: 70 767 167 545 5
Fragments arranged by size:
767 545 167 70 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1554
Size: 944 610
BsiHKAI G_wGCw'C
Cuts at: 0 976 1554
Size: 976 578
BsmI GAATG_Cn'
Cuts at: 0 646 1554
Size: 646 908
Bsp1286I G_dGCh'C
Cuts at: 0 176 378 399 725 904 964 976 1388
Size: 176 202 21 326 179 60 12 412
Cuts at: 1388 1554
Size: 166
Fragments arranged by size:
412 326 202 179 176 166 60 21
12
BspHI T'CATG_A
Cuts at: 0 1262 1554
Size: 1262 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 784 1554
Size: 784 770
BsrBI CCG'CTC
Cuts at: 0 498 1554
Size: 498 1056
BstEII G'GTnAC_C
Cuts at: 0 586 1554
Size: 586 968
BstYI r'GATC_y
Cuts at: 0 416 1013 1554
Size: 416 597 541
Fragments arranged by size:
597 541 416
Bsu36I CC'TnA_GG
Cuts at: 0 890 1554
Size: 890 664
BtgI C'CryG_G
Cuts at: 0 86 92 1554
Size: 86 6 1462
Fragments arranged by size:
1462 86 6
BtsI GCAGTG_nn'
Cuts at: 0 909 1298 1554
Size: 909 389 256
Fragments arranged by size:
909 389 256
ClaI AT'CG_AT
Cuts at: 0 731 1554
Size: 731 823
EaeI y'GGCC_r
Cuts at: 0 89 1295 1554
Size: 89 1206 259
Fragments arranged by size:
1206 259 89
EarI CTCTTCn'nnn_
Cuts at: 0 644 1174 1277 1554
Size: 644 530 103 277
Fragments arranged by size:
644 530 277 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 198 305 1198 1262 1310 1554
Size: 121 77 107 893 64 48 244
Fragments arranged by size:
893 244 121 107 77 64 48
EcoO109I rG'GnC_Cy
Cuts at: 0 395 396 654 721 722 767 848 1384
Size: 395 1 258 67 1 45 81 536
Cuts at: 1384 1385 1453 1490 1498 1554
Size: 1 68 37 8 56
Fragments arranged by size:
536 395 258 81 68 67 56 45
37 8 1 1 1
EcoRV GAT'ATC
Cuts at: 0 126 1554
Size: 126 1428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 534 1203 1554
Size: 534 669 351
Fragments arranged by size:
669 534 351
FspI TGC'GCA
Cuts at: 0 1393 1554
Size: 1393 161
HaeII r_GCGC'y
Cuts at: 0 800 1259 1554
Size: 800 459 295
Fragments arranged by size:
800 459 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 1554
Size: 1501 32 21
Fragments arranged by size:
1501 32 21
HincII GTy'rAC
Cuts at: 0 633 1064 1554
Size: 633 431 490
Fragments arranged by size:
633 490 431
KasI G'GCGC_C
Cuts at: 0 796 1554
Size: 796 758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 1034 1554
Size: 774 260 520
Fragments arranged by size:
774 520 260
MscI TGG'CCA
Cuts at: 0 1297 1554
Size: 1297 257
MslI CAynn'nnrTG
Cuts at: 0 623 969 1554
Size: 623 346 585
Fragments arranged by size:
623 585 346
MspA1I CmG'CkG
Cuts at: 0 743 1554
Size: 743 811
NarI GG'CG_CC
Cuts at: 0 797 1554
Size: 797 757
NspI r_CATG'y
Cuts at: 0 1103 1482 1554
Size: 1103 379 72
Fragments arranged by size:
1103 379 72
PasI CC'CwG_GG
Cuts at: 0 484 658 1554
Size: 484 174 896
Fragments arranged by size:
896 484 174
PciI A'CATG_T
Cuts at: 0 1478 1554
Size: 1478 76
PfoI T'CCnGG_A
Cuts at: 0 137 1554
Size: 137 1417
PpuMI rG'GwC_Cy
Cuts at: 0 654 848 1453 1498 1554
Size: 654 194 605 45 56
Fragments arranged by size:
654 605 194 56 45
PshAI GACnn'nnGTC
Cuts at: 0 853 1554
Size: 853 701
PsiI TTA'TAA
Cuts at: 0 197 1554
Size: 197 1357
PspOMI G'GGCC_C
Cuts at: 0 395 721 1384 1554
Size: 395 326 663 170
Fragments arranged by size:
663 395 326 170
PstI C_TGCA'G
Cuts at: 0 1073 1554
Size: 1073 481
SalI G'TCGA_C
Cuts at: 0 1062 1554
Size: 1062 492
SapI GCTCTTCn'nnn_
Cuts at: 0 1174 1554
Size: 1174 380
SfcI C'TryA_G
Cuts at: 0 1069 1554
Size: 1069 485
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 299 1554
Size: 299 1255
SfoI GGC'GCC
Cuts at: 0 798 1554
Size: 798 756
SmaI CCC'GGG
Cuts at: 0 1090 1495 1554
Size: 1090 405 59
Fragments arranged by size:
1090 405 59
SmlI C'TyrA_G
Cuts at: 0 930 1009 1554
Size: 930 79 545
Fragments arranged by size:
930 545 79
StuI AGG'CCT
Cuts at: 0 293 492 874 1134 1554
Size: 293 199 382 260 420
Fragments arranged by size:
420 382 293 260 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 574 910 1018 1554
Size: 328 246 336 108 536
Fragments arranged by size:
536 336 328 246 108
TatI w'GTAC_w
Cuts at: 0 464 1554
Size: 464 1090
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 481 665 811 1554
Size: 481 184 146 743
Fragments arranged by size:
743 481 184 146
XmaI C'CCGG_G
Cuts at: 0 1088 1493 1554
Size: 1088 405 61
Fragments arranged by size:
1088 405 61
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BstEII BstYI Bsu36I BtgI BtsI
ClaI EaeI EarI Eco57MI EcoO109I EcoRV FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NspI PasI PciI PfoI PpuMI PshAI PsiI
PspOMI PstI SalI SapI SfcI SfiI SfoI SmaI
SmlI StuI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SanDI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI