Restriction map for
pF1KB5960
(Linear) (Six-base) MAPSORT of: KIBB5960.seq Check: 3299 from: 1 to: 1653
KIBB5960.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1080 1302 1653
Size: 1080 222 351
Fragments arranged by size:
1080 351 222
AatII G_ACGT'C
Cuts at: 0 713 1653
Size: 713 940
AccI GT'mk_AC
Cuts at: 0 4 1370 1653
Size: 4 1366 283
Fragments arranged by size:
1366 283 4
AclI AA'CG_TT
Cuts at: 0 146 1653
Size: 146 1507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1653
Size: 337 1316
AflIII A'CryG_T
Cuts at: 0 296 1653
Size: 296 1357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1180 1653
Size: 1180 473
AlwNI CAG_nnn'CTG
Cuts at: 0 315 490 730 838 943 1459 1653
Size: 315 175 240 108 105 516 194
Fragments arranged by size:
516 315 240 194 175 108 105
ApaI G_GGCC'C
Cuts at: 0 103 760 962 1013 1355 1402 1591 1621
Size: 103 657 202 51 342 47 189 30
Cuts at: 1621 1653
Size: 32
Fragments arranged by size:
657 342 202 189 103 51 47 32
30
AvaI C'yCGr_G
Cuts at: 0 511 1614 1653
Size: 511 1103 39
Fragments arranged by size:
1103 511 39
BamHI G'GATC_C
Cuts at: 0 611 1653
Size: 611 1042
BanI G'GyrC_C
Cuts at: 0 20 1215 1653
Size: 20 1195 438
Fragments arranged by size:
1195 438 20
BanII G_rGCy'C
Cuts at: 0 103 499 760 962 1013 1127 1355 1402
Size: 103 396 261 202 51 114 228 47
Cuts at: 1402 1591 1621 1653
Size: 189 30 32
Fragments arranged by size:
396 261 228 202 189 114 103 51
47 32 30
BbeI G_GCGC'C
Cuts at: 0 24 1653
Size: 24 1629
BbsI GAAGACnn'nnnn_
Cuts at: 0 705 1336 1653
Size: 705 631 317
Fragments arranged by size:
705 631 317
BbvCI CC'TCA_GC
Cuts at: 0 1014 1271 1653
Size: 1014 257 382
Fragments arranged by size:
1014 382 257
BglI GCCn_nnn'nGGC
Cuts at: 0 1397 1653
Size: 1397 256
BlpI GC'TnA_GC
Cuts at: 0 380 1653
Size: 380 1273
Bme1580I G_kGCm'C
Cuts at: 0 103 760 962 1013 1355 1402 1591 1621
Size: 103 657 202 51 342 47 189 30
Cuts at: 1621 1653
Size: 32
Fragments arranged by size:
657 342 202 189 103 51 47 32
30
BmrI ACTGGGnnnn_n'
Cuts at: 0 680 1653
Size: 680 973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 815 906 1216 1433 1571 1653
Size: 547 268 91 310 217 138 82
Fragments arranged by size:
547 310 268 217 138 91 82
Bpu10I CC'TnA_GC
Cuts at: 0 498 1014 1271 1653
Size: 498 516 257 382
Fragments arranged by size:
516 498 382 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 427 1653
Size: 36 391 1226
Fragments arranged by size:
1226 391 36
BsaAI yAC'GTr
Cuts at: 0 333 1565 1653
Size: 333 1232 88
Fragments arranged by size:
1232 333 88
BsaBI GATnn'nnATC
Cuts at: 0 646 1653
Size: 646 1007
BsaHI Gr'CG_yC
Cuts at: 0 21 213 710 1333 1653
Size: 21 192 497 623 320
Fragments arranged by size:
623 497 320 192 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 283 289 313 511 541 673 703
Size: 259 24 6 24 198 30 132 30
Cuts at: 703 1293 1323 1653
Size: 590 30 330
Fragments arranged by size:
590 330 259 198 132 30 30 30
24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 565 604 619 667 697 1653
Size: 562 3 39 15 48 30 956
Fragments arranged by size:
956 562 48 39 30 15 3
BseYI C'CCAG_C
Cuts at: 0 723 759 936 949 1081 1452 1653
Size: 723 36 177 13 132 371 201
Fragments arranged by size:
723 371 201 177 132 36 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1653
Size: 328 1325
BsiEI CG_ry'CG
Cuts at: 0 1396 1653
Size: 1396 257
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1531 1653
Size: 1531 122
Bsp1286I G_dGCh'C
Cuts at: 0 103 499 760 962 1013 1127 1355 1402
Size: 103 396 261 202 51 114 228 47
Cuts at: 1402 1591 1621 1653
Size: 189 30 32
Fragments arranged by size:
396 261 228 202 189 114 103 51
47 32 30
BspHI T'CATG_A
Cuts at: 0 167 1653
Size: 167 1486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 638 1080 1302 1653
Size: 638 442 222 351
Fragments arranged by size:
638 442 351 222
BsrBI CCG'CTC
Cuts at: 0 429 609 1653
Size: 429 180 1044
Fragments arranged by size:
1044 429 180
BsrDI GCAATG_nn'
Cuts at: 0 93 1653
Size: 93 1560
BsrFI r'CCGG_y
Cuts at: 0 259 413 867 1035 1218 1391 1653
Size: 259 154 454 168 183 173 262
Fragments arranged by size:
454 262 259 183 173 168 154
BssSI C'ACGA_G
Cuts at: 0 81 1653
Size: 81 1572
BstEII G'GTnAC_C
Cuts at: 0 1067 1653
Size: 1067 586
BstYI r'GATC_y
Cuts at: 0 611 1053 1261 1653
Size: 611 442 208 392
Fragments arranged by size:
611 442 392 208
BtgI C'CryG_G
Cuts at: 0 198 473 767 785 973 1653
Size: 198 275 294 18 188 680
Fragments arranged by size:
680 294 275 198 188 18
BtsI GCAGTG_nn'
Cuts at: 0 289 1162 1653
Size: 289 873 491
Fragments arranged by size:
873 491 289
DrdI GACnn_nn'nnGTC
Cuts at: 0 1338 1653
Size: 1338 315
EaeI y'GGCC_r
Cuts at: 0 529 575 1393 1653
Size: 529 46 818 260
Fragments arranged by size:
818 529 260 46
EagI C'GGCC_G
Cuts at: 0 1393 1653
Size: 1393 260
EarI CTCTTCn'nnn_
Cuts at: 0 419 1653
Size: 419 1234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 532 1460 1653
Size: 73 459 928 193
Fragments arranged by size:
928 459 193 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 547 815 906 1216 1433 1571 1653
Size: 337 210 268 91 310 217 138 82
Fragments arranged by size:
337 310 268 217 210 138 91 82
EcoO109I rG'GnC_Cy
Cuts at: 0 99 502 756 879 907 932 958 996
Size: 99 403 254 123 28 25 26 38
Cuts at: 996 1010 1351 1399 1587 1588 1618 1653
Size: 14 341 48 188 1 30 35
Fragments arranged by size:
403 341 254 188 123 99 48 38
35 30 28 26 25 14 1
HaeII r_GCGC'y
Cuts at: 0 24 1558 1653
Size: 24 1534 95
Fragments arranged by size:
1534 95 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 673 705 1120 1152 1363 1395
Size: 259 32 382 32 415 32 211 32
Cuts at: 1395 1653
Size: 258
Fragments arranged by size:
415 382 259 258 211 32 32 32
32
HincII GTy'rAC
Cuts at: 0 5 918 1371 1653
Size: 5 913 453 282
Fragments arranged by size:
913 453 282 5
KasI G'GCGC_C
Cuts at: 0 20 1653
Size: 20 1633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1451 1653
Size: 1451 202
MscI TGG'CCA
Cuts at: 0 577 1653
Size: 577 1076
MslI CAynn'nnrTG
Cuts at: 0 172 1653
Size: 172 1481
MspA1I CmG'CkG
Cuts at: 0 387 490 542 567 835 949 1653
Size: 387 103 52 25 268 114 704
Fragments arranged by size:
704 387 268 114 103 52 25
NaeI GCC'GGC
Cuts at: 0 869 1220 1393 1653
Size: 869 351 173 260
Fragments arranged by size:
869 351 260 173
NarI GG'CG_CC
Cuts at: 0 21 1653
Size: 21 1632
NcoI C'CATG_G
Cuts at: 0 198 767 973 1653
Size: 198 569 206 680
Fragments arranged by size:
680 569 206 198
NgoMIV G'CCGG_C
Cuts at: 0 867 1218 1391 1653
Size: 867 351 173 262
Fragments arranged by size:
867 351 262 173
NspI r_CATG'y
Cuts at: 0 300 1653
Size: 300 1353
PasI CC'CwG_GG
Cuts at: 0 95 749 809 845 1058 1231 1653
Size: 95 654 60 36 213 173 422
Fragments arranged by size:
654 422 213 173 95 60 36
PciI A'CATG_T
Cuts at: 0 296 1653
Size: 296 1357
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 1459 1653
Size: 333 1126 194
Fragments arranged by size:
1126 333 194
PfoI T'CCnGG_A
Cuts at: 0 1174 1653
Size: 1174 479
PpuMI rG'GwC_Cy
Cuts at: 0 879 907 932 996 1653
Size: 879 28 25 64 657
Fragments arranged by size:
879 657 64 28 25
PspOMI G'GGCC_C
Cuts at: 0 99 756 958 1009 1351 1398 1587 1617
Size: 99 657 202 51 342 47 189 30
Cuts at: 1617 1653
Size: 36
Fragments arranged by size:
657 342 202 189 99 51 47 36
30
PstI C_TGCA'G
Cuts at: 0 560 641 906 948 1299 1653
Size: 560 81 265 42 351 354
Fragments arranged by size:
560 354 351 265 81 42
PvuII CAG'CTG
Cuts at: 0 387 490 567 835 949 1653
Size: 387 103 77 268 114 704
Fragments arranged by size:
704 387 268 114 103 77
SalI G'TCGA_C
Cuts at: 0 3 1369 1653
Size: 3 1366 284
Fragments arranged by size:
1366 284 3
SanDI GG'GwC_CC
Cuts at: 0 879 932 996 1653
Size: 879 53 64 657
Fragments arranged by size:
879 657 64 53
SapI GCTCTTCn'nnn_
Cuts at: 0 419 1653
Size: 419 1234
SbfI CC_TGCA'GG
Cuts at: 0 906 1653
Size: 906 747
SfcI C'TryA_G
Cuts at: 0 556 637 902 944 1295 1653
Size: 556 81 265 42 351 358
Fragments arranged by size:
556 358 351 265 81 42
SfoI GGC'GCC
Cuts at: 0 22 1653
Size: 22 1631
SmaI CCC'GGG
Cuts at: 0 1616 1653
Size: 1616 37
SmlI C'TyrA_G
Cuts at: 0 15 442 1653
Size: 15 427 1211
Fragments arranged by size:
1211 427 15
StuI AGG'CCT
Cuts at: 0 891 1423 1653
Size: 891 532 230
Fragments arranged by size:
891 532 230
StyI C'CwwG_G
Cuts at: 0 198 449 767 922 973 1418 1578 1653
Size: 198 251 318 155 51 445 160 75
Fragments arranged by size:
445 318 251 198 160 155 75 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 780 1163 1575 1653
Size: 780 383 412 78
Fragments arranged by size:
780 412 383 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 511 543 954 986 1293 1325 1653
Size: 511 32 411 32 307 32 328
Fragments arranged by size:
511 411 328 307 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 9 1653
Size: 9 1644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 456 1653
Size: 237 219 1197
Fragments arranged by size:
1197 237 219
XmaI C'CCGG_G
Cuts at: 0 1614 1653
Size: 1614 39
XmnI GAAnn'nnTTC
Cuts at: 0 322 1653
Size: 322 1331
ZraI GAC'GTC
Cuts at: 0 711 1653
Size: 711 942
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII
BstYI BtgI BtsI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PspOMI PstI PvuII SalI
SanDI SapI SbfI SfcI SfoI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaWI
BsiHKAI BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI